Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 515,827 T→A 5.5% M107L (ATG→TTG) ‡ hinT ← aminoacyl‑adenylate hydrolase/purine nucleoside phosphoramidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947515,8270TA 5.5% 224.0 / 2.4 72M107L (ATG→TTG) ‡hinTaminoacyl‑adenylate hydrolase/purine nucleoside phosphoramidase
Reads supporting (aligned to +/- strand):  ref base T (33/35);  new base A (2/2);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01

GGTATCGGCCTGCAGCAACAAGCGGTCGAGCGGCGAGTAGTGACGTTCGGTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGCCAGGCGCTGGGCGGTGAACAGGATGTGGCCAGCCAGGG  >  NC_002947/515685‑515973
                                                                                                                                              |                                                                                                                                                  
ggTATCGGCCTGCAGCAACAAGCGGTCGAGCGGCGAGTAGTGACGTTCGGTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGgcg                                                                                                                                              <  4:135385/149‑1 (MQ=255)
    tCGGCCTGCAGCAACAAGCGGTCGAGCGGCGAGTAGTGACGTTCGGTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGATGAACTGgcctccg                                                                                                                                          <  7:7005/149‑6 (MQ=255)
       gCCAGCAGCAACAAGCGGTCGAGCGGCGAGTAGAGACGTTCGGTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGcc                                                                                                                                        >  6:207215/1‑148 (MQ=255)
         cTGCAGCAACAAGCGGTCGAGCGGCGAGTAGTGACGTTCGGTAGCCATCGGGCACCTCAGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAg                                                                                                                                     >  2:200442/1‑149 (MQ=255)
          tGCAGCAACAAGCGGTCGAGCGGCGAGTAGTGACGTTCGGTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAg                                                                                                                                     >  5:222373/1‑148 (MQ=255)
            cagcaACAAGCGGTCGAGCGGCGAGTAGTGACGTTCGGTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGATGAACTGgc                                                                                                                                               <  8:205133/136‑1 (MQ=255)
            cagcaACAAGCGGTCGAGCGGCGAGTAGTGACGTTCGGTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGATGAACTGgc                                                                                                                                               >  7:205133/1‑136 (MQ=255)
             agcaACAAGCGGTCGAGCGGCGAGTAGTGACGTTCGGTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACg                                                                                                                                 <  8:254805/149‑1 (MQ=255)
               caacaaGCGGTCGAGCGGCGAGTAGTGACGTTCGGTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTg                                                                                                                               >  1:126381/1‑149 (MQ=255)
                 acaaGCGGTCGAGCGGCGAGTAGTGACGTTCGGTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCCGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCa                                                                                                                             >  4:199701/1‑149 (MQ=255)
                 acaaGCGGTCGAGCGGCGAGTAGTGACGTTCGGTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCa                                                                                                                             >  2:8380/1‑149 (MQ=255)
                              cggcgAGTAGTGACGTTCGGTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTgg                                                                                                                  >  8:134965/1‑147 (MQ=255)
                                gcgAGTAGTGACGTTCGGTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGa                                                                                                              <  7:134965/149‑1 (MQ=255)
                                gcgAGTAGTGACGTTCGGTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGa                                                                                                              >  2:239257/1‑149 (MQ=255)
                                        tGACGTTCGGTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTgg                                                                                                      >  6:266908/1‑149 (MQ=255)
                                          aCGTTCGGTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGgc                                                                                                     <  4:157366/148‑1 (MQ=255)
                                                ggTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCttt                                                                                              >  8:101541/1‑149 (MQ=255)
                                                 gTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTg                                                                                             >  2:176834/1‑149 (MQ=255)
                                                 gTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTg                                                                                             <  6:222373/149‑1 (MQ=255)
                                                  tAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGt                                                                                                                                <  8:188703/113‑1 (MQ=255)
                                                     ccATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGt                                                                                                          >  6:152459/1‑132 (MQ=255)
                                                     ccATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGt                                                                                                          <  5:152459/132‑1 (MQ=255)
                                                     ccATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGt                                                                                         >  5:254061/1‑149 (MQ=255)
                                                      cATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGgcc                                                                                                    <  6:286041/137‑1 (MQ=255)
                                                      cATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGgcc                                                                                                    >  5:286041/1‑137 (MQ=255)
                                                       aTCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTg                                                                                       <  5:207215/149‑1 (MQ=255)
                                                           ggCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGcc                                                                                                                                        <  6:230168/96‑1 (MQ=255)
                                                           ggCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGcc                                                                                                                                        >  5:230168/1‑96 (MQ=255)
                                                               ccTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATTTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCAt                                                                               <  7:101541/149‑1 (MQ=255)
                                                                cTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCAt                                                                               >  4:302827/1‑148 (MQ=255)
                                                                cTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATg                                                                              >  2:198277/1‑149 (MQ=255)
                                                                cTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATg                                                                              <  1:176834/149‑1 (MQ=255)
                                                                   cGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTAAGCCCGTAGGCCAGTTCATCTGGGGCTGGCCATGCACTTGCATATGGTTGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGAc                                                                            <  1:8701/148‑1 (MQ=255)
                                                                    gCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACca                                                                          >  2:282587/1‑149 (MQ=255)
                                                                     cAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGATTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGATGGCCCAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACca                                                                          >  7:178548/1‑148 (MQ=255)
                                                                      aaGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACcaca                                                                        >  4:269497/1‑149 (MQ=255)
                                                                      aaGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACcaca                                                                        >  6:127492/1‑149 (MQ=255)
                                                                            gtgCCCGACAGTTGACCGCAATAGCGGCGGGGTCTCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGaaa                                                                  <  7:92788/149‑1 (MQ=255)
                                                                             tgCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATg                                                                              >  6:136533/1‑136 (MQ=255)
                                                                             tgCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATg                                                                              <  5:136533/136‑1 (MQ=255)
                                                                                 cGACAGTTTACCGCAATAGCGGCGGGGGCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTc                                                             <  7:24693/149‑1 (MQ=255)
                                                                                  gACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGtt                                                                                        <  7:113219/121‑1 (MQ=255)
                                                                                  gACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGtt                                                                                        >  8:113219/1‑121 (MQ=255)
                                                                                  gACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTcc                                                            <  5:107399/149‑1 (MQ=255)
                                                                                   aCAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCt                                                           >  4:295363/1‑149 (MQ=255)
                                                                                       tttACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTgg                                                                                                      <  2:177519/102‑1 (MQ=255)
                                                                                       tttACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTgg                                                                                                      >  1:177519/1‑102 (MQ=255)
                                                                                       tttACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCg                                                         >  5:20891/1‑147 (MQ=255)
                                                                                          aCCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGACAAGCTCGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGc                                                     <  5:99681/148‑1 (MQ=255)
                                                                                           ccGCAATAGCGGCGGGGTCGCGTGCCGTGGGTCAGCCCGGATGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGcc                                                    <  8:178548/148‑1 (MQ=255)
                                                                                              cAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGt                                                <  5:266908/149‑1 (MQ=255)
                                                                                              cAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGt                                                >  8:34100/1‑149 (MQ=255)
                                                                                               aaTAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGtt                                               <  1:200442/149‑1 (MQ=255)
                                                                                                      gcggGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGc                                        <  7:34100/149‑1 (MQ=255)
                                                                                                      gcggGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGc                                        <  1:239257/149‑1 (MQ=255)
                                                                                                            tCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCATACGGAAACCTTCCTCGCAGCCTTGTTCGACAGCCAGgcg                                  >  5:60561/1‑149 (MQ=255)
                                                                                                                   ccGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGCCAGGCGCTGGGc                            <  3:302827/148‑1 (MQ=255)
                                                                                                                   ccGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGCCAGGCGCTGGGc                            <  1:198277/148‑1 (MQ=255)
                                                                                                                     gTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGCCAGGCGCTGGGCGGt                         <  3:269497/149‑1 (MQ=255)
                                                                                                                     gTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGCCAGGCGCTGGGCGGt                         >  3:288230/1‑149 (MQ=255)
                                                                                                                     gTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGCCAGGCGCTGGGCGGt                         <  3:295363/149‑1 (MQ=255)
                                                                                                                        ggTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGCCAGGCGCTGGGCGGTGaa                      <  1:8380/149‑1 (MQ=255)
                                                                                                                         gTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGCCAGGCGCTggg                             >  7:88797/1‑141 (MQ=255)
                                                                                                                         gTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGCCAGGCGCTggg                             <  8:88797/141‑1 (MQ=255)
                                                                                                                                cGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGCCAGGCGCTGGGCGGTGAACa                    >  2:115486/1‑143 (MQ=255)
                                                                                                                                cGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGCCAGGCGCTGGGCGGTGAACa                    <  1:115486/143‑1 (MQ=255)
                                                                                                                                   gcgCCAGATGAACTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGCCAGGCGCTGGGCGGTGAACAGGATGTGgc           >  1:77363/3‑149 (MQ=255)
                                                                                                                                     gCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGCCAGGCGCTGGGCGGTGAACAGGATGTGgc           >  3:205890/1‑147 (MQ=255)
                                                                                                                                        aGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGCCAGGCGCTGGGCGGTGAACAGGATGTGgccagcc      <  4:288230/149‑1 (MQ=255)
                                                                                                                                             aTCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGCCAGGCGCTGGGCGGTGAACAGGATGTGGccagccag    >  3:112986/1‑146 (MQ=255)
                                                                                                                                             aTCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGCCAGGCGCTGGGCGGTGAACAGGATGTGGccagccag    <  4:112986/146‑1 (MQ=255)
                                                                                                                                             aTCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGCCAGGCGCTGGGCGGTGAACAGGATGTGGCCAGCCAggg  <  5:127492/148‑1 (MQ=255)
                                                                                                                                              tCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACa                                          >  6:185167/1‑107 (MQ=255)
                                                                                                                                              tCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACa                                          <  5:185167/107‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
GGTATCGGCCTGCAGCAACAAGCGGTCGAGCGGCGAGTAGTGACGTTCGGTAGCCATCGGGCACCTCCGCAAGAATGTGCCCGACAGTTTACCGCAATAGCGGCGGGGTCGCTTGCCGTGGGTCAGCCCGGAGGCCAGTTCATCTGGCGCTGGCCAAGCACGTGCATATGGATGTGGTAGACGGTCTGGCCGCCTTTGGGGTTGCAGTTCATGACCACACGGAAACCTTCCTCGCAGCCTTGTTCGACAGCCAGGCGCTGGGCGGTGAACAGGATGTGGCCAGCCAGGG  >  NC_002947/515685‑515973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑