Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,044,249 C→T 10.7% V347V (GTG→GTA)  PP_5297 ← amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,044,2490CT10.7% 150.5 / 7.3 56V347V (GTG→GTA) PP_5297amino acid transporter
Reads supporting (aligned to +/- strand):  ref base C (24/26);  new base T (3/3);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-01

ATGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGTGAACAGG  >  NC_002947/6044109‑6044396
                                                                                                                                            |                                                                                                                                                   
aTGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGAt                                                                                                                                             <  2:55688/149‑1 (MQ=255)
aTGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGAt                                                                                                                                             <  6:194156/149‑1 (MQ=255)
  ggtggCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTGCTGGCCAGTACGGCgg                                                                                                                                              <  2:72407/146‑2 (MQ=255)
  ggtggCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTGCTGGCCAGTACGGCgg                                                                                                                                              >  1:72407/1‑145 (MQ=255)
  ggtggCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATg                                                                                                                                            <  2:208496/148‑1 (MQ=255)
                   tttGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGTGATGGACATCAg                                                                                                                                    <  1:277518/139‑1 (MQ=255)
                   tttGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGAGATGGACATCAg                                                                                                                                    >  2:277518/1‑139 (MQ=255)
                     tGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCg                                                                                                                         <  3:196576/148‑1 (MQ=255)
                              gggCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTGCTGGCCAGTACGGCg                                                                                                                                               <  3:57448/117‑1 (MQ=255)
                              gggCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTGCTGGCCAGTACGGCg                                                                                                                                               >  4:57448/1‑117 (MQ=255)
                              gggCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAAACCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGAGACGCCGCCGAACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGAc                                                                                                               >  6:155411/1‑149 (MQ=255)
                                  aGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCggg                                                                                                           <  2:150143/149‑1 (MQ=255)
                                  aGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCgg                                                                                                            <  8:135014/148‑1 (MQ=255)
                                  aGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCgg                                                                                                            >  7:135014/1‑148 (MQ=255)
                                    acacTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGggcc                                                                                                         <  8:110878/149‑1 (MQ=255)
                                       cTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGggccggc                                                                                                      <  1:225868/149‑1 (MQ=255)
                                        tGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATc                                                                                                                                <  6:64183/122‑1 (MQ=255)
                                        tGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATc                                                                                                                                >  5:64183/1‑122 (MQ=255)
                                          gCGAACTTCTGCGGTACTGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAg                                                                                                   >  3:57356/1‑149 (MQ=255)
                                            gAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGt                                                                                                 >  2:1384/1‑149 (MQ=255)
                                                ttCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTggg                                                                                              >  3:177331/1‑148 (MQ=255)
                                                     cGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGa                                                                                        >  7:14399/1‑149 (MQ=255)
                                                             cACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTcc                                                                                >  4:18236/1‑149 (MQ=255)
                                                             cACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTcc                                                                                <  8:14399/149‑1 (MQ=255)
                                                                cGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTg                                                                              >  3:262376/1‑148 (MQ=255)
                                                                  taataaCCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGAt                                                                           <  3:267109/149‑1 (MQ=255)
                                                                         ccATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCggg                                                                    >  8:11364/1‑149 (MQ=255)
                                                                         ccATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCggg                                                                    >  1:148423/1‑149 (MQ=255)
                                                                         ccATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATAGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCggg                                                                    >  7:192420/1‑149 (MQ=255)
                                                                           aTGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCg                                                                                                             <  7:144610/106‑1 (MQ=255)
                                                                           aTGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCg                                                                                                             >  8:144610/1‑106 (MQ=255)
                                                                           aTGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCaaa                                                                                          <  5:331526/125‑1 (MQ=255)
                                                                           aTGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCaaa                                                                                          >  6:331526/1‑125 (MQ=255)
                                                                              gCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGgttgtt                                                               <  2:148423/149‑1 (MQ=255)
                                                                              gCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGgttgtt                                                               <  1:1384/149‑1 (MQ=255)
                                                                               ccAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGgttgtt                                                               >  5:178479/1‑148 (MQ=255)
                                                                                              ccGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCggt                                               >  8:144031/1‑147 (MQ=255)
                                                                                                  cgcCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCAc                                           <  4:177331/149‑1 (MQ=255)
                                                                                                   gcCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCCGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACt                                          >  6:89364/1‑149 (MQ=255)
                                                                                                         aaGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATAcc                                    <  3:270297/149‑1 (MQ=255)
                                                                                                                    cAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGcc                                                         <  2:24859/117‑1 (MQ=255)
                                                                                                                    cAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGcc                                                         >  1:24859/1‑117 (MQ=255)
                                                                                                                    cAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCa                         <  3:18236/149‑1 (MQ=255)
                                                                                                                      gCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAgg                       >  4:24323/1‑149 (MQ=255)
                                                                                                                       cGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAg                                                            <  7:228360/111‑1 (MQ=255)
                                                                                                                       cGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAg                                                            >  8:228360/1‑111 (MQ=255)
                                                                                                                       cGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAggg                      >  5:163076/1‑149 (MQ=255)
                                                                                                                         aCGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTg                    <  6:318627/149‑1 (MQ=255)
                                                                                                                         aCGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTg                    <  4:262376/149‑1 (MQ=255)
                                                                                                                          cgccgcCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTgg                   <  4:57356/149‑1 (MQ=255)
                                                                                                                           gccgccGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGc                  >  1:123180/1‑149 (MQ=255)
                                                                                                                            tcgccgTGCTGGCCAGTACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGc                  >  1:136393/2‑148 (MQ=255)
                                                                                                                              gacgTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGTCAAAGACGTTCTCCTGTATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGAt               <  5:155411/147‑1 (MQ=255)
                                                                                                                                     tGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGTg        <  8:192420/149‑1 (MQ=255)
                                                                                                                                       gCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGTGa       >  3:202975/1‑148 (MQ=255)
                                                                                                                                         cAGTACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGTGAACa    <  2:136393/149‑1 (MQ=255)
                                                                                                                                            cACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGTGAACAgg  >  8:191961/1‑148 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
ATGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGCACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGTGAACAGG  >  NC_002947/6044109‑6044396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑