Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,051,669 C→G 12.0% intergenic (+18/‑39) ridA → / → PP_5304 aminoacrylate/iminopropionate hydrolase/deaminase/lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,051,6690CG12.0% 135.3 / 10.6 50intergenic (+18/‑39)ridA/PP_5304aminoacrylate/iminopropionate hydrolase/deaminase/lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (19/25);  new base G (2/4);  total (21/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

ACTTCGCCAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAGCCATCA  >  NC_002947/6051521‑6051813
                                                                                                                                                    |                                                                                                                                                
aCTTCGCCAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGccc                                                                                                                                                  >  3:88301/1‑149 (MQ=255)
 cTTCGCCAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCt                                                                                                                                                 >  1:323841/1‑149 (MQ=255)
     gCCAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGccc                                                                                                                                             >  3:142307/1‑149 (MQ=255)
     gCCAAGATAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGccc                                                                                                                                             >  6:279573/1‑149 (MQ=255)
          ggTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCGGGTGACGGGCAGGGCCCGTCAc                                                                                                                                        <  6:309017/149‑1 (MQ=255)
           gTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCAcc                                                                                                                                       <  1:147420/149‑1 (MQ=255)
            tAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCAGGGCCCGTCAcca                                                                                                                                      <  5:277005/149‑2 (MQ=255)
              aaCGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCAGGGCCCGTCAccag                                                                                                                                     <  2:3688/148‑3 (MQ=255)
               aCGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCTGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGACCAGGTCGAAATGGCCGCCATCCTGGTCATCGAATGATGCCCTGGTGACTGGCCCTGCCCGTCACcctcc                                                                                                                                   <  6:13940/149‑1 (MQ=255)
                 gAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATAGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCtcctt                                                                                                                                 >  8:285798/1‑149 (MQ=255)
                       aTGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCTTCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCctct                                                                                                                            <  5:279573/148‑1 (MQ=255)
                          ggCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGTTGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCaa                                                                                                                        <  4:205334/149‑1 (MQ=255)
                           gCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAg                                                                                                                       <  1:124941/149‑1 (MQ=255)
                                      gAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTTCAAGGTTACCCCGt                                                                                                             <  3:89974/148‑1 (MQ=255)
                                       aaCAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACTCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCa                                                                                                           <  2:323841/149‑1 (MQ=255)
                                        aCAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCAt                                                                                                          <  4:142307/149‑1 (MQ=255)
                                             ccTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCCCCCTCCTTCCTCTGCAACTGAACCCCGTCATGCGt                                                                                                      >  6:226420/1‑148 (MQ=255)
                                               tACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGc                                                                                                        <  7:87705/144‑1 (MQ=255)
                                               tACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGc                                                                                                        >  8:87705/1‑144 (MQ=255)
                                                   cGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTCCCCCGTCATGCGTCAAGCAc                                                                                               >  7:91079/1‑149 (MQ=255)
                                                   cGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCAc                                                                                               <  7:59165/149‑1 (MQ=255)
                                                       ccGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGcc                                                                                           >  5:28746/1‑149 (MQ=255)
                                                                          gCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCt                                                                         <  7:285798/148‑1 (MQ=255)
                                                                              cACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTAGCAGCCCTGCTAc                                                                    <  5:295720/149‑1 (MQ=255)
                                                                                 tGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCAt                                                                                                          >  6:97821/1‑108 (MQ=255)
                                                                                 tGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCAt                                                                                                          <  5:97821/108‑1 (MQ=255)
                                                                                   ccGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGggc                                                               <  4:88301/149‑1 (MQ=255)
                                                                                   ccGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGggc                                                               >  1:99220/1‑149 (MQ=255)
                                                                                       atggCGTCCATTTCGACCTGGGCGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCt                                                           >  2:85466/3‑149 (MQ=255)
                                                                                        tggCGTCCATTTCGACCTGGGCGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTg                                                          <  1:85466/148‑1 (MQ=255)
                                                                                         ggCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGc                                                         <  8:91079/149‑1 (MQ=255)
                                                                                             cccAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGggcgg                                                             <  7:146233/141‑1 (MQ=255)
                                                                                             cccAGGTCGAAATGGACGCCATCCTGCTCATCGAATGATGCCCTGGTGAAGGGCCCTGCCCCTCACCCTCCTTCCTCTGCAACGTTACACCGTCATGCGTCAAGAACTGCCCTACGCGCTCGCAGCCATTCTACTGcgcgg                                                             >  8:146233/1‑141 (MQ=255)
                                                                                              ccAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAg                                                    <  8:175637/149‑1 (MQ=255)
                                                                                              ccAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAg                                                    >  7:175637/1‑149 (MQ=255)
                                                                                                      aaaTGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAAc                                            >  5:254725/1‑149 (MQ=255)
                                                                                                               ccATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGAtt                                   <  7:299094/149‑1 (MQ=255)
                                                                                                                cATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGAttt                                  >  1:105851/1‑149 (MQ=255)
                                                                                                                                      gagggTGACGGGCAGGGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAAc                              <  6:165328/128‑1 (MQ=255)
                                                                                                                                      gagggTGACGGGCAGGGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAAc                              >  5:165328/4‑131 (MQ=255)
                                                                                                                                       cTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGAt                                    >  4:239087/1‑124 (MQ=255)
                                                                                                                                       cTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGAt                                    <  3:239087/124‑1 (MQ=255)
                                                                                                                                        tGGTGACGTGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGGACCAGCCACAAAACCGAAGTTTTCAACGGCATCTGGATCAACCa             <  1:274934/146‑1 (MQ=255)
                                                                                                                                          gTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCATCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAg        >  7:225406/1‑149 (MQ=255)
                                                                                                                                          gTGACGGGCAGGGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTCCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGAGTTCAACGGCATCTGGATCAACCAGGaac        >  7:225909/1‑148 (MQ=255)
                                                                                                                                           tGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAGc       <  5:221806/149‑1 (MQ=255)
                                                                                                                                              cGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAGCCAt    <  2:105851/149‑1 (MQ=255)
                                                                                                                                               gggCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCa             >  7:273045/1‑139 (MQ=255)
                                                                                                                                               gggCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCa             <  8:273045/139‑1 (MQ=255)
                                                                                                                                               gggCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAGCCAt    >  8:93255/1‑148 (MQ=255)
                                                                                                                                                ggCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAGCCATCa  <  2:99220/149‑1 (MQ=255)
                                                                                                                                                    cTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGAt                   <  1:139281/128‑1 (MQ=255)
                                                                                                                                                    cTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGAt                   >  2:139281/1‑128 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
ACTTCGCCAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAGCCATCA  >  NC_002947/6051521‑6051813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑