Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,051,670 T→G 11.8% intergenic (+19/‑38) ridA → / → PP_5304 aminoacrylate/iminopropionate hydrolase/deaminase/lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,051,6700TG11.8% 134.2 / 11.4 51intergenic (+19/‑38)ridA/PP_5304aminoacrylate/iminopropionate hydrolase/deaminase/lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (19/26);  new base G (2/4);  total (21/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.31e-01

CTTCGCCAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAGCCATCACTGCG  >  NC_002947/6051522‑6051818
                                                                                                                                                    |                                                                                                                                                    
cTTCGCCAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCt                                                                                                                                                      >  1:323841/1‑149 (MQ=255)
    gCCAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGccc                                                                                                                                                  >  3:142307/1‑149 (MQ=255)
    gCCAAGATAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGccc                                                                                                                                                  >  6:279573/1‑149 (MQ=255)
         ggTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCGGGTGACGGGCAGGGCCCGTCAc                                                                                                                                             <  6:309017/149‑1 (MQ=255)
          gTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCAcc                                                                                                                                            <  1:147420/149‑1 (MQ=255)
           tAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCAGGGCCCGTCAcca                                                                                                                                           <  5:277005/149‑2 (MQ=255)
             aaCGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCAGGGCCCGTCAccag                                                                                                                                          <  2:3688/148‑3 (MQ=255)
              aCGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCTGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGACCAGGTCGAAATGGCCGCCATCCTGGTCATCGAATGATGCCCTGGTGACTGGCCCTGCCCGTCACcctcc                                                                                                                                        <  6:13940/149‑1 (MQ=255)
                gAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATAGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCtcctt                                                                                                                                      >  8:285798/1‑149 (MQ=255)
                      aTGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCTTCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCctct                                                                                                                                 <  5:279573/148‑1 (MQ=255)
                         ggCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGTTGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCaa                                                                                                                             <  4:205334/149‑1 (MQ=255)
                          gCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAg                                                                                                                            <  1:124941/149‑1 (MQ=255)
                                     gAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTTCAAGGTTACCCCGt                                                                                                                  <  3:89974/148‑1 (MQ=255)
                                      aaCAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACTCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCa                                                                                                                <  2:323841/149‑1 (MQ=255)
                                       aCAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCAt                                                                                                               <  4:142307/149‑1 (MQ=255)
                                            ccTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCCCCCTCCTTCCTCTGCAACTGAACCCCGTCATGCGt                                                                                                           >  6:226420/1‑148 (MQ=255)
                                              tACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGc                                                                                                             <  7:87705/144‑1 (MQ=255)
                                              tACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGc                                                                                                             >  8:87705/1‑144 (MQ=255)
                                                  cGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTCCCCCGTCATGCGTCAAGCAc                                                                                                    >  7:91079/1‑149 (MQ=255)
                                                  cGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCAc                                                                                                    <  7:59165/149‑1 (MQ=255)
                                                      ccGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGcc                                                                                                >  5:28746/1‑149 (MQ=255)
                                                                         gCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCt                                                                              <  7:285798/148‑1 (MQ=255)
                                                                             cACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTAGCAGCCCTGCTAc                                                                         <  5:295720/149‑1 (MQ=255)
                                                                                tGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCAt                                                                                                               >  6:97821/1‑108 (MQ=255)
                                                                                tGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCAt                                                                                                               <  5:97821/108‑1 (MQ=255)
                                                                                  ccGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGggc                                                                    >  1:99220/1‑149 (MQ=255)
                                                                                  ccGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGggc                                                                    <  4:88301/149‑1 (MQ=255)
                                                                                      atggCGTCCATTTCGACCTGGGCGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCt                                                                >  2:85466/3‑149 (MQ=255)
                                                                                       tggCGTCCATTTCGACCTGGGCGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTg                                                               <  1:85466/148‑1 (MQ=255)
                                                                                        ggCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGc                                                              <  8:91079/149‑1 (MQ=255)
                                                                                            cccAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGggcgg                                                                  <  7:146233/141‑1 (MQ=255)
                                                                                            cccAGGTCGAAATGGACGCCATCCTGCTCATCGAATGATGCCCTGGTGAAGGGCCCTGCCCCTCACCCTCCTTCCTCTGCAACGTTACACCGTCATGCGTCAAGAACTGCCCTACGCGCTCGCAGCCATTCTACTGcgcgg                                                                  >  8:146233/1‑141 (MQ=255)
                                                                                             ccAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAg                                                         <  8:175637/149‑1 (MQ=255)
                                                                                             ccAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAg                                                         >  7:175637/1‑149 (MQ=255)
                                                                                                     aaaTGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAAc                                                 >  5:254725/1‑149 (MQ=255)
                                                                                                              ccATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGAtt                                        <  7:299094/149‑1 (MQ=255)
                                                                                                               cATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGAttt                                       >  1:105851/1‑149 (MQ=255)
                                                                                                                                     gagggTGACGGGCAGGGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAAc                                   <  6:165328/128‑1 (MQ=255)
                                                                                                                                     gagggTGACGGGCAGGGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAAc                                   >  5:165328/4‑131 (MQ=255)
                                                                                                                                      cTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGAt                                         >  4:239087/1‑124 (MQ=255)
                                                                                                                                      cTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGAt                                         <  3:239087/124‑1 (MQ=255)
                                                                                                                                       tGGTGACGTGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGGACCAGCCACAAAACCGAAGTTTTCAACGGCATCTGGATCAACCa                  <  1:274934/146‑1 (MQ=255)
                                                                                                                                         gTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCATCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAg             >  7:225406/1‑149 (MQ=255)
                                                                                                                                         gTGACGGGCAGGGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTCCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGAGTTCAACGGCATCTGGATCAACCAGGaac             >  7:225909/1‑148 (MQ=255)
                                                                                                                                          tGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAGc            <  5:221806/149‑1 (MQ=255)
                                                                                                                                             cGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAGCCAt         <  2:105851/149‑1 (MQ=255)
                                                                                                                                              gggCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCa                  >  7:273045/1‑139 (MQ=255)
                                                                                                                                              gggCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCa                  <  8:273045/139‑1 (MQ=255)
                                                                                                                                              gggCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAGCCAt         >  8:93255/1‑148 (MQ=255)
                                                                                                                                               ggCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAGCCATCa       <  2:99220/149‑1 (MQ=255)
                                                                                                                                                   cTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGAt                        >  2:139281/1‑128 (MQ=255)
                                                                                                                                                   cTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGAt                        <  1:139281/128‑1 (MQ=255)
                                                                                                                                                    tGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAGCCATCACTgcg  >  5:41791/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTTCGCCAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAGCCATCACTGCG  >  NC_002947/6051522‑6051818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑