Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 516,282 A→T 8.1% L237Q (CTG→CAG)  PP_0429 ← short‑chain dehydrogenase/reductase family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947516,2820AT 8.1% 218.7 / 7.3 74L237Q (CTG→CAG) PP_0429short‑chain dehydrogenase/reductase family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (38/30);  new base T (4/2);  total (42/32)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-01

GTCCACAGCACTTGCTCCATGGTGAGGGTCGGGCCGAGTGTACTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAGCGCGGCTTGCCATCGAGCCGCGCAATGCGCCGGCGTGC  >  NC_002947/516140‑516430
                                                                                                                                              |                                                                                                                                                    
gTCCACAGCACTTGCTCCATGGTGAGGGTCGGGCCGAGTGTACTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCcgcg                                                                                                                                                <  7:42730/149‑1 (MQ=255)
  ccACAGCACTTGCTCCATGGTGAGGGTCGGGCCGAGTGTACTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGt                                                                                                                                              >  2:83504/1‑149 (MQ=255)
   cacaGCACTTGCTCCATGGTGAGGGTCGGGCCGAGTGTACTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTg                                                                                                                                             >  2:212725/1‑149 (MQ=255)
    acaGCACTTGCTCCATGGTGAGGGTCGGGCCGAGTGTACTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGc                                                                                                                                            >  1:34498/1‑149 (MQ=255)
     caGCACTTGCTCCATGGTGAGGGTCGGGCCGAGTGTACTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCg                                                                                                                                           <  2:34498/149‑1 (MQ=255)
        cACTTGCTCCATGGTGAGGGTCGGGCCGAGTGTACTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCTGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGc                                                                                                                                            >  5:277769/1‑145 (MQ=255)
        cACTTGCTCCATGGTGAGGGTCGGGCCGAGTGTACTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGc                                                                                                                                            <  6:277769/145‑1 (MQ=255)
              cTCCATGGTGAGGGTCGGGCCGAGTGTACTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACgccagc                                                                                                                                  >  7:271125/1‑149 (MQ=255)
                     gTGAGGGTCGGGCCGAGTGTACTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGt                                                                                                                           >  6:240401/1‑149 (MQ=255)
                          ggTCGTGCCGAGTGTACTCAGCCGGGAGCCGCTCGCCCAGTGTCTATTCCAAGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTgcgc                                                                                                                      <  4:232905/149‑1 (MQ=255)
                                gCCGAGTGTACTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACgcca                                                                                                                                    <  8:273101/129‑1 (MQ=255)
                                gCCGAGTGTACTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACgcca                                                                                                                                    >  7:273101/1‑129 (MQ=255)
                                gCCGAGTGTACTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCgccggccg                                                                                                                >  5:127045/1‑149 (MQ=255)
                                 ccgcgctgcctcagccccgaggaagtggcgctgtacaccgtgcgggcactggacaagaaccgcgcactgatcatgCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGccggccgg                                                                                                               >  3:130087‑M1/76‑149 (MQ=255)
                                   gAGTGTACTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCggg                                                                                                              >  7:141240/1‑148 (MQ=255)
                                   gAGTGTACTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCggg                                                                                                              >  2:52508/1‑148 (MQ=255)
                                    aGTGTACTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCACCGCGGCTGCTGCCGCGCTggctgg                                                                                                                                        >  1:290803/1‑116 (MQ=255)
                                    aGTGTACTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCACCGCGGCTGCTGCCGCGCTggctgg                                                                                                                                        <  2:290803/121‑6 (MQ=255)
                                     gctgcctcagccccgaggaagtggcgctgtacaccgtgcgggcactggacaagaaccgcgcactgatcatgCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCAt                                                                                                           >  8:289306‑M1/72‑149 (MQ=255)
                                        tACTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGAt                                                                                                        <  8:135406/149‑1 (MQ=255)
                                         cctcagccccgaggaagtggcgctgtacaccgtgcgggcactggacaagaaccgcgcactgatcatgCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCCGCGGTTGCGCCGGCCGGGCATGAt                                                                                                        >  1:146444‑M1/68‑148 (MQ=255)
                                          cTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCa                                                                                                      >  3:205055/1‑149 (MQ=255)
                                           tCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAg                                                                                                     >  7:245074/1‑149 (MQ=255)
                                           tCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAg                                                                                                     >  7:181941/1‑149 (MQ=255)
                                           tCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAg                                                                                                     >  8:35895/1‑149 (MQ=255)
                                                      ccGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCt                                                                                          >  8:278845/1‑149 (MQ=255)
                                                        gCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTg                                                                                        <  1:83504/149‑1 (MQ=255)
                                                            gCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTc                                                                                      <  5:240401/147‑1 (MQ=255)
                                                            gCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTc                                                                                      <  4:205055/147‑1 (MQ=255)
                                                            gCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTc                                                                                      >  4:54398/1‑147 (MQ=255)
                                                                    gCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCg                                                                             >  2:226128/1‑148 (MQ=255)
                                                                     cTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCa                                                                           >  8:54963/1‑149 (MQ=255)
                                                                     cTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCa                                                                           >  4:19681/1‑149 (MQ=255)
                                                                        ttCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCAc                                                                          <  8:271125/147‑1 (MQ=255)
                                                                        ttCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCAc                                                                          <  1:183961/147‑1 (MQ=255)
                                                                         tCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGccc                                                                              >  2:155884/1‑142 (MQ=255)
                                                                         tCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGccc                                                                              <  1:155884/142‑1 (MQ=255)
                                                                          ccATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCCGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGgt                                                                       <  1:226128/148‑1 (MQ=255)
                                                                           cATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGgtg                                                                      >  3:305727/1‑148 (MQ=255)
                                                                           cATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGgtg                                                                      <  8:141240/148‑1 (MQ=255)
                                                                              gCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCa                                                                                                      <  6:285719/113‑1 (MQ=255)
                                                                              gCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCa                                                                                                      >  5:285719/1‑113 (MQ=255)
                                                                               ccTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAg                                                                 <  7:54963/149‑1 (MQ=255)
                                                                                   aGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAgcg                                                               <  8:245074/147‑1 (MQ=255)
                                                                                          aTAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCt                                                      <  1:212725/149‑1 (MQ=255)
                                                                                          aTAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCt                                                      >  7:45327/1‑149 (MQ=255)
                                                                                             gcggcgGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCg                                                    <  6:289427/148‑1 (MQ=255)
                                                                                                 cggTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCt                                               >  4:101025/1‑149 (MQ=255)
                                                                                                      gATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGa                                             >  1:216279/1‑146 (MQ=255)
                                                                                                      gATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGa                                             <  2:216279/146‑1 (MQ=255)
                                                                                                       aTGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCa                                         <  1:52508/149‑1 (MQ=255)
                                                                                                          gCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCgg                                                   >  6:270095/1‑136 (MQ=255)
                                                                                                           ccccGGCCAGGTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAgcgc                                     <  6:302071/149‑1 (MQ=255)
                                                                                                           ccccGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGa                                             <  4:248369/141‑1 (MQ=255)
                                                                                                           ccccGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGa                                             >  3:248369/1‑141 (MQ=255)
                                                                                                             CCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGTGCATGATCAGTGCGCGGTTCTTGTCCAGt                                                                                  >  1:78846‑M1/1‑102 (MQ=255)
                                                                                                             CCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGCGCATGATCAGTGCGCGGTTCTTGTCCAGt                                                                                  <  2:78846‑M1/102‑1 (MQ=255)
                                                                                                                 CCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAGCGCGGCTTg                               >  3:54398‑M1/1‑149 (MQ=255)
                                                                                                                   aGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAGCGCGGCTTGc                              >  5:247130/1‑148 (MQ=255)
                                                                                                                        gcgcACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAGCGCGGCTTGCCATc                          >  2:146521/1‑147 (MQ=255)
                                                                                                                        gcgcACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAGCGCGGCTTGCCATc                          >  6:278602/1‑147 (MQ=255)
                                                                                                                           cACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAGCGCGGCTTGCCATCGAGcc                     <  8:181941/149‑1 (MQ=255)
                                                                                                                            aCCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAGCGCGGCTTGCCATCGAGCcg                    >  1:116810/1‑149 (MQ=255)
                                                                                                                              cagccagcGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAGCGCGGCTTGCCATCGAGCcgcg                  <  8:45327/149‑1 (MQ=255)
                                                                                                                               agccagcGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAGCGCGGCTTGCCATCGAGCcgcg                  >  8:82449/1‑148 (MQ=255)
                                                                                                                                 ccagcGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAgg                                           <  7:118367/121‑1 (MQ=255)
                                                                                                                                 ccagcGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAgg                                           >  8:118367/1‑121 (MQ=255)
                                                                                                                                  cagcGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAGCGCGGCTTGCCATCGAGCCGCGCAAt              <  6:305455/149‑1 (MQ=255)
                                                                                                                                  cagcGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGGGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAGCGCGGCTTGCCATCGAGCCGCGCAAt              <  6:310029/149‑1 (MQ=255)
                                                                                                                                  caccgcgGCTGCTGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAGCGCGGCtt                                >  6:212847/4‑131 (MQ=255)
                                                                                                                                  caccgcgGCTGCTGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAGCGCGGCtt                                <  5:212847/128‑1 (MQ=255)
                                                                                                                                    gcgcGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAGCGCGGCTTGCCATCGAGCCGCGCAATgc            <  4:305727/149‑1 (MQ=255)
                                                                                                                                    ccgcgGCTGCTGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAGCGCGGCTTGCCATCGAGCCGCGCAATgc            >  7:278830/2‑149 (MQ=255)
                                                                                                                                        ggCTGCTGCCGCGCTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAGCGCGGCTTGCCATCGAGCCGCGCAATGCGCCg        >  1:290042/1‑149 (MQ=255)
                                                                                                                                          cagcagCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAGCGCGGCTTGCCATCGAGCCGCGCAATGCGCCGGc      <  6:247130/149‑1 (MQ=255)
                                                                                                                                              agcCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAGCGCGGCTTGCCATCGAGCCGCGCAATGCGCCGGCGTGc  <  8:40665/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
GTCCACAGCACTTGCTCCATGGTGAGGGTCGGGCCGAGTGTACTCAGCCGTGAGCCGCTCGCCCAGTGGCTATTCCATGCCTGAGGGGCAATAGCGGCGGTGGATGGCCCCGGCCAGCTTGCGCACCAGCCAGCGCGGCAGCAGCCGCGGTGCGAACGCCAGCCAGCGGTTGCGCCGGCCGGGCATGATCAGTGCGCGGTTCTTGTCCAGTGCCCGCACGGTGTACAGCGCCACTTCCTCGGGGCTGAGGCAGCGCGGCTTGCCATCGAGCCGCGCAATGCGCCGGCGTGC  >  NC_002947/516140‑516430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑