Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,080,145 A→T 5.2% Q30L (CAG→CTG)  PP_5332 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,080,1450AT 5.2% 235.4 / 2.7 77Q30L (CAG→CTG) PP_5332hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (38/35);  new base T (2/2);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

AACTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCAGCGCAACC  >  NC_002947/6080001‑6080291
                                                                                                                                                |                                                                                                                                                   
aaCTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCcagcag                                                                                                                                                 <  3:73690/149‑1 (MQ=255)
 aCTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCATTGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAgg                                                                                                                                                <  6:79689/149‑1 (MQ=255)
  cTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAggg                                                                                                                                               >  5:102714/1‑149 (MQ=255)
   tCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGgc                                                                                                                                              >  3:269629/1‑149 (MQ=255)
      ttGCATCTGACCGGGTCCAAGGCCCACTGTCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAAGATGTCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGgcgga                                                                                                                                           <  8:61443/149‑3 (MQ=255)
        gCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGcac                                                                                                                                          <  1:159694/148‑1 (MQ=255)
                 cGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAg                                                                                                                                >  5:184521/1‑149 (MQ=255)
                        aaGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCTTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAg                                                                                                                                        <  6:210589/134‑1 (MQ=255)
                        aaGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAg                                                                                                                                        >  5:210589/1‑134 (MQ=255)
                         aGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTc                                                                                                                                  <  8:13712/139‑1 (MQ=255)
                         aGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTc                                                                                                                                  >  7:13712/1‑139 (MQ=255)
                         aGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACcag                                                                                                                         >  5:256471/1‑148 (MQ=255)
                          ggCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACcag                                                                                                                         >  8:251488/1‑147 (MQ=255)
                               aCTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAg                                                                                                                  <  8:176522/149‑1 (MQ=255)
                                       tcaatGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGTGCGCCCTGCTGGgcgatca                                                                                                                                         >  8:150046/1‑114 (MQ=255)
                                       tcaatGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGTGCGCCCTGCTGGgcgatca                                                                                                                                         <  7:150046/118‑5 (MQ=255)
                                         aatGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAAccgcc                                                                                                        >  1:323064/1‑149 (MQ=255)
                                             agaagaACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCgctg                                                                                                    >  7:155656/1‑149 (MQ=255)
                                                 gaaCGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCtgt                                                                                                >  2:223321/1‑149 (MQ=255)
                                                 gaaCGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCtgt                                                                                                <  1:59734/149‑1 (MQ=255)
                                                 gaaCGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCtgt                                                                                                <  7:173498/149‑1 (MQ=255)
                                                 gaaCGCAATGGCTACCTTTCAGGTCCTAACCGAGCAACATGGCGAACTCAACTGCTGGCGAATCAGCAGTGACCGAGCCGAACTCCAGATCGCCAAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCcctcctct                                                                                                >  8:165310/1‑147 (MQ=255)
                                                  aaCGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCca                                                                                                                                                     <  6:140163/95‑1 (MQ=255)
                                                  aaCGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCca                                                                                                                                                     >  5:140163/1‑95 (MQ=255)
                                                   aCGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTgg                                                                                              <  5:44662/149‑1 (MQ=255)
                                                   aCGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTgg                                                                                              <  5:270834/149‑1 (MQ=255)
                                                      cAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCt                                                                                            >  3:293624/1‑148 (MQ=255)
                                                       aaTGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCt                                                                                            >  7:140959/1‑147 (MQ=255)
                                                       aaTGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCt                                                                                            >  2:155747/1‑147 (MQ=255)
                                                       aaTGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGa                                                                                          <  6:102714/149‑1 (MQ=255)
                                                                 tttCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGc                                                                               >  1:124989/1‑149 (MQ=255)
                                                                  ttCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGc                                                                               >  6:223587/1‑148 (MQ=255)
                                                                   tCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGcc                                                                              <  2:323064/148‑1 (MQ=255)
                                                                    cAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATAGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCAt                                                                            >  2:241990/1‑149 (MQ=255)
                                                                          gTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTcc                                                                       <  7:251488/148‑1 (MQ=255)
                                                                              ccGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAg                                                                  >  5:312677/1‑149 (MQ=255)
                                                                               cGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAgg                                                                 <  8:140959/149‑1 (MQ=255)
                                                                                 aGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAggg                                                                >  2:149495/1‑148 (MQ=255)
                                                                                     aCATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGt                                                           >  8:263219/1‑149 (MQ=255)
                                                                                      cATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTc                                                          >  2:177362/1‑149 (MQ=255)
                                                                                       aTGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCg                                                         <  6:184521/149‑1 (MQ=255)
                                                                                        tGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCgg                                                        <  4:269629/149‑1 (MQ=255)
                                                                                          gCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCACACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGt                                                       <  8:212035/148‑1 (MQ=255)
                                                                                             aaCTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTAcgc                                                   >  4:87268/1‑149 (MQ=255)
                                                                                                tCAACTGCTGGTGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGc                                                                     >  2:183948/1‑128 (MQ=255)
                                                                                                tCAACTGCTGGTGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGc                                                                     <  1:183948/128‑1 (MQ=255)
                                                                                                tCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGcc                                                >  5:317539/1‑149 (MQ=255)
                                                                                                  aaCTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCg                                               <  6:256471/148‑1 (MQ=255)
                                                                                                       ctgGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACTAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTAc                                         <  6:136806/149‑1 (MQ=255)
                                                                                                       ctgGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTAc                                         >  2:8961/1‑149 (MQ=255)
                                                                                                        tgGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTAcc                                        >  5:121076/1‑149 (MQ=255)
                                                                                                          gCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCg                                       <  5:223587/148‑1 (MQ=255)
                                                                                                           cGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGgt                                     <  1:177362/149‑1 (MQ=255)
                                                                                                           cGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGgt                                     <  7:104729/149‑1 (MQ=255)
                                                                                                            gTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGACTCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGgt                                     <  1:241990/149‑1 (MQ=255)
                                                                                                            gTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGCCCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGgtg                                    >  5:185791/1‑149 (MQ=255)
                                                                                                            gTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTcc                                                                       >  7:47603/1‑114 (MQ=255)
                                                                                                            gTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTcc                                                                       <  8:47603/114‑1 (MQ=255)
                                                                                                               tCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGtgtg                                  <  1:8961/148‑1 (MQ=255)
                                                                                                                cagcagTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCt                                >  1:95752/1‑149 (MQ=255)
                                                                                                                 agcagTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTg                               <  1:155747/149‑1 (MQ=255)
                                                                                                                         ccGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGt                       >  2:115470/1‑149 (MQ=255)
                                                                                                                           gTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGttt                     <  7:165310/149‑1 (MQ=255)
                                                                                                                           gTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGttt                     <  6:317539/149‑1 (MQ=255)
                                                                                                                             gCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTgg                   >  1:302161/1‑149 (MQ=255)
                                                                                                                              ccGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTgg                   <  6:312677/148‑1 (MQ=255)
                                                                                                                                  aCTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAAcc              <  2:302161/149‑1 (MQ=255)
                                                                                                                                    acctgtgcGCCCTGCTGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTg            >  5:44785/8‑149 (MQ=255)
                                                                                                                                      cTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGtgtg                                  <  7:268817/125‑1 (MQ=255)
                                                                                                                                      cTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGtgtg                                  >  8:268817/1‑125 (MQ=255)
                                                                                                                                      cTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAAc               <  7:175378/144‑1 (MQ=255)
                                                                                                                                      cTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAAc               >  8:175378/1‑144 (MQ=255)
                                                                                                                                      cTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTg            >  4:53676/1‑147 (MQ=255)
                                                                                                                                        gATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTAc                                                     <  1:211932/104‑1 (MQ=255)
                                                                                                                                        gATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTAc                                                     >  2:211932/1‑104 (MQ=255)
                                                                                                                                         ggcGCCCTGCTGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCAgcg       <  6:44785/147‑1 (MQ=255)
                                                                                                                                              ccAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCAGCGCa     >  1:319036/1‑146 (MQ=255)
                                                                                                                                              ccAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCAGCGCa     <  2:319036/146‑1 (MQ=255)
                                                                                                                                              ccAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCAGCGCAAcc  >  2:66440/1‑149 (MQ=255)
                                                                                                                                              ccAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCAGCGCAAcc  >  6:106215/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
AACTCATTGCATCTGCCCGGGTCCAAGGCCCACTGGCAATCAATGAGAAGAACGCAATGGCTACCTTTCAGGTCGTAACCGAGCAACATGGCGAACTCAACTGCTGGCGTATCAGCAGTGACCGTGCCGAACTCCTGATCGCCCAGCAGGGCGCACAGGTCCTCAGCTACCAGCAGGTAGGCGAACCGCCGCTGCTGTGGCTGA‑GCGACCAGGCCATCTTCCGCCAGGGCAAGTCGGTACGCGCCGGGGTACCGGTGTGCTGGCCATGGTTTGGCAACCTGCAGCGCAACC  >  NC_002947/6080001‑6080291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑