Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,085,276	A→T	14.8%	H129L (CAC→CTC)	aspA →	aspartate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,085,276	0	A	T	14.8%	95.3 / 9.3	41	H129L (CAC→CTC)	aspA	aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base A (16/18);  new base T (2/4);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GCTGCCATTTCCGCAGCCTGCGCCCGCCTGATCAAAGGCGATTTCCACGACCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGACCAACGACGCCTACCCGACCGCAATCCGCCTGGGCCTGCTGCTGGGCCACGACGCGCTGCTGGCCAGCCTTGATAGCCTGATCCAGGC  >  NC_002947/6085128‑6085423                                                                                                                                                      |                                                                                                                                                    gCTGCCATTTCCGCAGCCTGCGCCCGCCTGATCAAAGGCGATTTCCACGACCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGcca                                                                                                                                                     >  4:103722/1‑149 (MQ=255)  cTGCCATTTCCGCAGCCTGCGCCCGCCTGATCAAAGGCGATTTCCACGACCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGccac                                                                                                                                                    <  3:70216/149‑1 (MQ=255)   tGCCATTTCCGCAGCCTGCGCCCGCCTGATCAAAGGCGATTTCCACGACCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGccac                                                                                                                                                    >  2:97751/1‑148 (MQ=255)     ccATTTCCGCAGCCTGCGCCCGCCTGATCAAAGGCGATTTCCACGACCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGTGGCCCATGGCCTCCAg                                                                                                                                                 >  2:292770/1‑149 (MQ=255)      cATTTCCGCAGCCTGCGCCCGCCTGATCAAAGGCGATTTCCACGACCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGTGGCCCATGGCCTCCAgc                                                                                                                                                <  1:292770/149‑2 (MQ=255)      cATTTCCGCAGCCTGCGCCCGCCTGATCAAAGGCGATTTCCACGACCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGTGGCCCATGGCCTCCAgc                                                                                                                                                <  7:246891/149‑2 (MQ=255)       aTTTCCGCAGCCTGCGCCCGCCTGATCAAAGGCGATTTCCACGACCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAg                                                                                                                                                 >  5:189845/1‑147 (MQ=255)        tttCCGCAGCCTGCGCCCGCCTGATCAAAGGCGATTTCCACGACCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGATCGTACTTCTACAAACATTAACGCCAAAGAACTCATTGCCAACGTTGCGCTGGAGGCCATTGGccaccac                                                                                                                                                 >  7:149871/1‑145 (MQ=255)            cGCAGCCTGCGCCCGCCTGATCAAAGGCGATTTCCACGACCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGt                                                                                                                                           <  1:97751/148‑1 (MQ=255)               aGCCTGCGCCCGCCTGATCAAAGGCGATTTCCACGACCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGagt                                                                                                                                       <  5:319570/149‑1 (MQ=255)                                tCAAAGGCGATTTCCACGACCAGTTCGTGGTAGAGCATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGagtacc                                                                                                                                    >  1:205830/1‑136 (MQ=255)                                tCAAAGGCGATTTCCACGACCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGagtacc                                                                                                                                    <  2:205830/135‑1 (MQ=255)                                tCAAAGGCGATTTCCACGACCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCAccc                                                                                                                       >  1:24717/1‑148 (MQ=255)                                 cAAAGGCGATTTCCACGACCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCt                                                                                                                      >  7:233053/1‑148 (MQ=255)                                      gCGATTTCCACGACCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTaacaac                                                                                                                >  3:128328/1‑149 (MQ=255)                                          ctaccACGAACTGGTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCGCCAGAAGGGTGAGTACCAGTACCTGCAc                                                                                                                         >  5:277291/4‑136 (MQ=255)                                          ctaccACGAACTGGTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCAc                                                                                                                         <  6:277291/133‑1 (MQ=255)                                              cACGACCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGTGGCCCATGGCCTCCAgc                                                                                                                                                >  6:89197/1‑108 (MQ=255)                                              cACGACCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGTGGCCCATGGCCTCCAgc                                                                                                                                                <  5:89197/109‑2 (MQ=255)                                              cACGACCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGa                                                                                                         <  4:31152/148‑1 (MQ=255)                                                  aCCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTAATTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATg                                                                                                    <  8:149871/149‑1 (MQ=255)                                                     aGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTTCGCTGGAGCCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGgcg                                                                                                 <  1:302634/149‑1 (MQ=255)                                                          gTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTc                                                                                            <  5:246512/149‑1 (MQ=255)                                                          gTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTc                                                                                            >  6:246512/1‑149 (MQ=255)                                                            ggTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGCCCATGGCCCACCAGAAGGGTGACTACCAGTACCTGCACCCAAACAACGACGAGAACATGGCGCAGTCGa                                                                                          >  5:68273/1‑149 (MQ=255)                                                             gTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGa                                                                                          <  3:103722/148‑1 (MQ=255)                                                                gA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGACCaa                                                                                      <  6:189845/149‑1 (MQ=255)                                                                      gATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGACCAacgacg                                                                                 >  4:33207/1‑149 (MQ=255)                                                                               cggcgCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGTGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGACCAACGACGCCTACCCGa                                                                        <  4:212442/149‑1 (MQ=255)                                                                                       gTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGACCAACGACGCCTACCCGACCGCaa                                                                  <  1:186229/147‑1 (MQ=255)                                                                                         aCTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGACCAACGACGCCTACACGACCGCAATACg                                                              >  7:241289/1‑149 (MQ=255)                                                                                              tACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGACCAACGACGCCTACCCGACCGCAATCCGCCTgg                                                         <  3:33207/149‑1 (MQ=255)                                                                                                ccAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGACCAACGACGCCTACCCGACCGCAATCCGCCTGGGc                                                       <  2:24717/149‑1 (MQ=255)                                                                                                   aCATGAACGCCAAAGAAGTCATTGCCAACGTTGCGCTGGAGGGCATGGGCCATCAGAAGGGTGAGTATCATTACCTGCACCCTATCAACGAGTTTTACATGGGGCAGTCGACCATCGACGCCTACCCGACCGCAATCCGCCTGGGCctg                                                    <  6:68273/149‑1 (MQ=255)                                                                                                             cAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGACCAACGACGCCTACCCGACCGCAATCCGCCTGGGCCTGCTGCTGGGcc                                          >  3:128953/1‑149 (MQ=255)                                                                                                                       aTTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGACCAACGACGCCTACCCGACCGCAATCCGCCTGGGCCTGCTGCTGGGCCACGACgcgc                                 <  3:96786/148‑1 (MQ=255)                                                                                                                            cAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGACCAACGACGCCTACCCGACCGCAATCCGCCTGGGCCTGCTGCTGGGCCACGACGCGCTGCTgg                           >  6:292203/1‑149 (MQ=255)                                                                                                                                 ttGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGACCAACGACGCCTACCCGACCGCAATCCGCCTGGGCCTGCTGCTGGGCCACGACGCGCTGCTGGCCAGc                      >  3:233022/1‑149 (MQ=255)                                                                                                                                    cgcTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGACCAACGACGCCTACCCGACCGCAATCCGCCTGGGCCTGCTGCTGGGCCACGACGCGCTGCTGGCCAGCCt                    <  5:292203/148‑1 (MQ=255)                                                                                                                                         gAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGACCAACGACGCCTACCCGACCGCAATCCGCCTGGGCCTGCTGCTGGGCCACGACGCGCTGCTGGCCAGCCTTGATAg              >  4:292435/1‑149 (MQ=255)                                                                                                                                          aGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGACCAACGACGCCTACCCGACCGCAATCCGCCTGGGCCTGCTGCTGGGCCACGACGCGCTGCTGGCCAGCCTTGATAGc             >  4:115101/1‑149 (MQ=255)                                                                                                                                              cATGGGCCACCAGAAGGGTGAGTACCAGTCCCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGACCAACGAAGCCTACCCGACCGCAATCCGCCTGGGCCTGCTGCTGGGCCACGACGCGCTGCTGGCCAGCCTTGATAGCCTGa         >  7:80002/1‑149 (MQ=255)                                                                                                                                               catggCCTCCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGACCAACGACGCCTACCCGACCGCAATCCGCCTGGGCCTGCTGCTGGGCCACGACGCGCTGCTGGCCAGCCTTGATAGCCTGAt        <  5:194644/146‑1 (MQ=255)                                                                                                                                                 gggCCACCAGAATGGTGAGTACCAGTACCTGCACCCTAACAACGTCGTGAACATGGCGCAGTCGACCAACGACGCCTACCCGACCGCAATCCGCCTGGGCCTTCTGCTGGGCCACGACGCGCTGCTGGCCAGCCt                    <  6:71805/135‑1 (MQ=255)                                                                                                                                                 gggCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTCAACATGGCGCAGTCGACCAACGACGCCTACCCGACCGCAATCCGCCTGGGCCTGCTGCTGGGCCACGACGCGCTGCTGGCCAGCCt                    >  5:71805/1‑135 (MQ=255)                                                                                                                                                    ccaccaGAAGGGTCAGTACCAGTACCTGCACCCTAACAACGACGTCAACATGGCGCAGTCGACCAACGACCCCTACCCGACCGCAATACGCCTGCGCCTGCAGCAGGGCCACGACGCGCTGCTGCCCAGCCTTGATAGCCTGATCCAgg   >  3:65742/1‑149 (MQ=255)                                                                                                                                                     caccaGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGACCAACGACGCCTACCCGACCGCAATCCGCCTGGGCCTGCTGCTGGGCCACGACGCGCTGCTGGCCAGCCTTGATAGCCTGATCCAGGc  >  6:229164/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                    GCTGCCATTTCCGCAGCCTGCGCCCGCCTGATCAAAGGCGATTTCCACGACCAGTTCGTGGTAGA‑CATGATCCAGGGCGGCGCTGGTACTTCTACCAACATGAACGCCAACGAAGTCATTGCCAACGTTGCGCTGGAGGCCATGGGCCACCAGAAGGGTGAGTACCAGTACCTGCACCCTAACAACGACGTGAACATGGCGCAGTCGACCAACGACGCCTACCCGACCGCAATCCGCCTGGGCCTGCTGCTGGGCCACGACGCGCTGCTGGCCAGCCTTGATAGCCTGATCCAGGC  >  NC_002947/6085128‑6085423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑