Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 517,460 G→C 6.4% L186V (CTG→GTG)  PP_0430 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947517,4600GC 6.4% 195.7 / 3.0 62L186V (CTG→GTG) PP_0430hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (33/25);  new base C (2/2);  total (35/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

GCCGGACACCACGGGTGTGTGGATGATGTCGGCGGCATGGGCATCCAGCAGCATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGGCCGCCCGCGCCTGCGGCGACGGCGATAAGGCCATCGACACCGGCT  >  NC_002947/517314‑517608
                                                                                                                                                  |                                                                                                                                                    
gCCGGACACCACGGGTGTGTGGATGATGTCGGCGGCATGGGCATCCAGCAGCATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGa                                                                                                                                                    >  7:254435/1‑149 (MQ=255)
         cacGGGTGTGTGGATGATGTCGGCGGCATGGGCATCCAGCAGCATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGc                                                                                                                                           >  2:135136/1‑149 (MQ=255)
            gggTGTGTGGATGATGTCGGCGGCATGGCCATCCAGCAGCATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGt                                                                                                                                        >  1:180716/1‑149 (MQ=255)
               tgtgtgGATGATGTCGGCGGCATGGGCATCCAGCAGCATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGt                                                                                                                                     <  1:256629/149‑1 (MQ=255)
                   tgGATGATGTCGGCGGCATGGGCATCCAGCAGCATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAAt                                                                                                                                 <  6:114674/149‑1 (MQ=255)
                       tgatgTCGGCGGCATGGGCATCCAGCAGCATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCaa                                                                                                                             <  5:209529/149‑1 (MQ=255)
                        gatgTCGGCGGCATGGGCATCCAGCAGCATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAAc                                                                                                                            >  6:25394/1‑149 (MQ=255)
                          tgTCGGCGGCATGGGCATCCAGCAGCATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCACGAAATCCTGGCCgc                                                                                                                                        >  8:267563/1‑134 (MQ=255)
                          tgTCGGCGGCATGGGCATCCAGCAGCATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCACGAAATCCTGGCCgc                                                                                                                                        <  7:267563/135‑2 (MQ=255)
                                       ggCATCCAGCAGCATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAggg                                                                                                             >  6:149621/1‑149 (MQ=255)
                                         cATCCAGCAGCATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAAccagcca                                                                                                                      <  2:233737/138‑1 (MQ=255)
                                         cATCCAGCAGCATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGATGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAAccagcca                                                                                                                      >  1:233737/1‑138 (MQ=255)
                                             cagcagCATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTg                                                                                                        <  5:149621/148‑1 (MQ=255)
                                             cagcagCATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGt                                                                                                       >  1:19793/1‑149 (MQ=255)
                                              agcagcATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAgg                                                                                                                                                     <  1:177259/102‑1 (MQ=255)
                                              agcagcATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAgg                                                                                                                                                     >  2:177259/1‑102 (MQ=255)
                                              agcagcATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTTATGCAACCAGCGAGCAGCAGGGTCTTGTc                                                                                                      >  4:4691/1‑149 (MQ=255)
                                              agcagcATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTc                                                                                                      >  4:253008/1‑149 (MQ=255)
                                               gcagcaTCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAgg                                                                                                              <  3:220518/140‑1 (MQ=255)
                                               gcagcaTCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAgg                                                                                                              >  4:220518/1‑140 (MQ=255)
                                               gcagcaTCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCg                                                                                                     <  2:180716/149‑1 (MQ=255)
                                                 agcaTCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCgaa                                                                                                   <  8:254435/149‑1 (MQ=255)
                                                  gcaTCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCg                                                                                                     >  5:230296/1‑146 (MQ=255)
                                                  gcaTCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCg                                                                                                     >  7:271290/1‑146 (MQ=255)
                                                     tCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAAc                                                                                               >  5:41777/1‑149 (MQ=255)
                                                     tCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGAAGAAGCGCGTGCCCATGTACGCCAGGTACGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAAc                                                                                               >  1:223536/1‑149 (MQ=255)
                                                         tttGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAAc                                                                                               <  5:25394/145‑1 (MQ=255)
                                                                gcgcCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTc                                                                                    >  5:11324/1‑149 (MQ=255)
                                                                   cccTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGc                                                                                 <  8:271290/149‑1 (MQ=255)
                                                                                    tgtgGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGc                                                                <  1:135136/149‑1 (MQ=255)
                                                                                     gtggtgGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCt                                                               >  4:279319/1‑149 (MQ=255)
                                                                                     gtggtgGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTATGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAAccagcca                                                                                                                      <  8:65308/94‑1 (MQ=255)
                                                                                     gtggtgGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTATGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAAccagcca                                                                                                                      >  7:65308/1‑94 (MQ=255)
                                                                                      tggtggCGATGAAGCGCGTGCCCATGTAAGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTc                                                              >  1:18703/1‑149 (MQ=255)
                                                                                         tggCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTcc                                                             <  5:178226/147‑1 (MQ=255)
                                                                                         tggCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTcc                                                             >  6:178226/1‑147 (MQ=255)
                                                                                          ggCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAgg                                                          >  5:167193/1‑149 (MQ=255)
                                                                                                gAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCgg                                                    >  7:291742/1‑149 (MQ=255)
                                                                                                gAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCg                                                     <  2:223536/148‑1 (MQ=255)
                                                                                                        tGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGgccg                                            <  2:18703/149‑1 (MQ=255)
                                                                                                         gCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGgccgc                                           >  3:135791/1‑149 (MQ=255)
                                                                                                          cccATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGgccgcc                                          >  6:254883/1‑149 (MQ=255)
                                                                                                           ccATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGgccgc                                           <  6:316660/147‑1 (MQ=255)
                                                                                                           ccATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGgccgc                                           >  5:316660/1‑147 (MQ=255)
                                                                                                           ccATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCACGATTTCGTGGCCGTgg                                                                                                                                      <  2:229049/56‑1 (MQ=255)
                                                                                                           ccATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCACGATTGCGTGGCCGTgg                                                                                                                                      >  1:229049/1‑56 (MQ=255)
                                                                                                              tGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGgccgc                                           <  4:206759/144‑1 (MQ=255)
                                                                                                              tGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGgccgc                                           >  3:206759/1‑144 (MQ=255)
                                                                                                                 aCGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGGCCGCCCGCGCCt                                   >  6:139471/1‑149 (MQ=255)
                                                                                                                    ccAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGGCCGCCCGCGCCTgcg                                >  5:111161/1‑149 (MQ=255)
                                                                                                                     cAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGGCCGCCCGCGCCTgcgg                               <  6:41777/149‑1 (MQ=255)
                                                                                                                      aGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGGCCGCCCGCGCCTgcggc                              <  6:167193/149‑1 (MQ=255)
                                                                                                                      aGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGGCCGCCCGCGCCTgcggc                              >  7:300746/1‑149 (MQ=255)
                                                                                                                           cGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGGCCGCCCGCGCCTGcggcgacgg                         <  6:230296/149‑1 (MQ=255)
                                                                                                                                ccAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCAt                                                 >  7:251502/1‑120 (MQ=255)
                                                                                                                                ccAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCAt                                                 <  8:251502/120‑1 (MQ=255)
                                                                                                                                  agcagTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGGCCGCCCGCGCCTGCGGCGACGGCGATAAg                  <  3:279319/149‑1 (MQ=255)
                                                                                                                                          tgCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGGCCGCCCGCGCCTGCGGCGACGGCGATAAGGCCATCGa          >  4:209198/1‑149 (MQ=255)
                                                                                                                                            cGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGGCCGCCCGCGCCTGCGGCGACGGCGATAAGGCCATCGaca        <  4:135791/149‑1 (MQ=255)
                                                                                                                                             gtgcAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGGAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCAGCGGCCAGGGGGAACGGGCTCCAGGTGCCGGCATGGCCGCCCGCGCCTGCGGCGACGGCGATAAGGCCATCGacac       <  6:11324/146‑1 (MQ=255)
                                                                                                                                             ggCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGGCCGCCCGCGCCTGCGGCGACGGCGATAAGGCCATCGacac       >  6:112774/1‑149 (MQ=255)
                                                                                                                                              gCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGGCCGCCCGCGCCTGCGGCGACGGCGATAAGGCCATCGACAcc      >  6:209884/1‑149 (MQ=255)
                                                                                                                                                cAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGGCCGCCCGCGCCTGCGGCGACGGCGATAAGGcc               >  7:91034/1‑138 (MQ=255)
                                                                                                                                                cAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGGCCGCCCGCGCCTGCGGCGACGGCGATAAGGcc               <  8:91034/138‑1 (MQ=255)
                                                                                                                                                  ggATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGGCCGCCCGCGCCTGCGGCGACGGCGATAAGGCCATCGACACCGGCt  <  3:209198/149‑1 (MQ=255)
                                                                                                                                                  ggATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGGCCGCCCGCGCCTGCGGCGACGGCGATAAGGCCATCGACACCGGCt  >  8:57333/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCCGGACACCACGGGTGTGTGGATGATGTCGGCGGCATGGGCATCCAGCAGCATCTGTTTGTAGGCGCCCTGAGCCTGGCTTTCTGTGGTGGCGATGAAGCGCGTGCCCATGTACGCCAGGTCCGCCCCCAGCAGTTGTGCGGCCAGGATTTCGTGGCCGTGGTTAATGCAACCAGCCAGCAGCAGGGTCTTGTCGAAGAACTGGCGAATTTCTGCGGCCAGGGCGAACGGGCTCCAGGTGCCGGCATGGCCGCCCGCGCCTGCGGCGACGGCGATAAGGCCATCGACACCGGCT  >  NC_002947/517314‑517608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑