Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,180,012 C→T 6.4% R36H (CGC→CAC)  atpF ← ATP synthase subunit b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,180,0120CT 6.4% 182.5 / 2.6 62R36H (CGC→CAC) atpFATP synthase subunit b
Reads supporting (aligned to +/- strand):  ref base C (28/30);  new base T (2/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

CTGAGCAGCGCGTTTCTTGCTTTGCTCGATGATTTCGGCTGCTTGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAGGGTTGCATTAATGTTCACGCCAACACCTCGCTCGGTCTTAATTCATACAGCCATC  >  NC_002947/6179864‑6180155
                                                                                                                                                    |                                                                                                                                               
cTGAGCAGCGCGTTTCTTGCTTTGCTCGATGATTTCGGCTGCTTGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGgc                                                                                                                                                 <  3:52314/149‑1 (MQ=255)
     cagcGCGTTTCTTGCTTTGCTCGATGATTTCGGCTGCTTGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGctct                                                                                                                                            >  1:322999/1‑149 (MQ=255)
       gcgcGTTTCTTGCTTTGCTCGATGATTTCGGCTGCTTGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGG‑CAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGc                                                                                                                                         <  2:118292/149‑1 (MQ=255)
         gcgTTTCTTGCTTTGCTCGATGATTTCGGCTGCTTGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCa                                                                                                                                        <  2:322999/149‑1 (MQ=255)
                ttgctttgctCGATGATTTCGGCTGCTTGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGt                                                                                                                                 >  4:115827/1‑149 (MQ=255)
                   ctttgctCGATGATTTCGGCTGCTTGTGCTTTAGATTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGtgat                                                                                                                              <  1:72814/149‑1 (MQ=255)
                    tttgctCGATGATTTCGGCTGCTTGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGtgat                                                                                                                              >  7:44285/1‑148 (MQ=255)
                    tttgctCGATGATTTCGGCTGCTTGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGtgat                                                                                                                              >  2:189543/1‑148 (MQ=255)
                         tCGATGATTTCGGCTGCTTGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGAc                                                                                                                           >  1:122985/1‑146 (MQ=255)
                         tCGATGATTTCGGCTGCTTGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGAc                                                                                                                           <  2:122985/146‑1 (MQ=255)
                           gatgatTTCGGCTGCTTGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGc                                                                                                                                         >  6:182117/1‑130 (MQ=255)
                           gatgatTTCGGCTGCTTGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGc                                                                                                                                         <  5:182117/130‑1 (MQ=255)
                           gatgatTTCGGCTGCTTGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAg                                                                                                                      >  5:260776/1‑149 (MQ=255)
                           gatgatTTCGGCTGCTTGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAg                                                                                                                      >  6:118990/1‑149 (MQ=255)
                               atTTCGGCTGCTTGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGcc                                                                                                                   <  6:260776/148‑1 (MQ=255)
                                      ctgctTGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGTGCTCTTGCAGAGCAGTGATGACCGGAGGCCatacatac                                                                                                           >  5:279330/1‑149 (MQ=255)
                                         ctTGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCaga                                                                                                                                      >  4:123511/1‑119 (MQ=255)
                                         ctTGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCaga                                                                                                                                      <  3:123511/119‑1 (MQ=255)
                                          ttGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTc                                                                                                        >  1:137175/1‑148 (MQ=255)
                                           tgtgCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCAt                                                                                                      >  2:10155/1‑149 (MQ=255)
                                            gtgCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTc                                                                                                        <  2:137175/146‑1 (MQ=255)
                                            gtgCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCa                                                                                                       <  5:97663/147‑1 (MQ=255)
                                             tgCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATg                                                                                                     <  5:325074/148‑1 (MQ=255)
                                             tgCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGc                                                                                                    <  8:231493/149‑1 (MQ=255)
                                                  tAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCagaa                                                                                                <  6:140074/148‑1 (MQ=255)
                                                     cTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGt                                                                                            <  3:115827/149‑1 (MQ=255)
                                                         aCGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGTGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACa                                                                                         <  6:279330/148‑1 (MQ=255)
                                                               ttGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCa                                                                                  <  4:154630/149‑1 (MQ=255)
                                                                tGCTTACCCGCTTTATCTTGGGCCAGCTCTAGTTCGCGAGGTGCGCGTTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCa                                                                                  <  8:71352/148‑1 (MQ=255)
                                                                       ccGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGc                                                                           >  7:271062/1‑148 (MQ=255)
                                                                            ttCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGa                                                                     >  5:294907/1‑149 (MQ=255)
                                                                               tctTGGGCCAGCTCCAGGTCGCGAGCTGCGGGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTg                                                                  >  1:28533/1‑149 (MQ=255)
                                                                               tctTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGctc                                                                                                                                             <  6:19010/74‑1 (MQ=255)
                                                                               tctTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGctc                                                                                                                                             >  5:19010/1‑74 (MQ=255)
                                                                                 ttGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGc                                                                >  3:38749/1‑149 (MQ=255)
                                                                                  tGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGc                                                                >  8:87562/1‑148 (MQ=255)
                                                                                   gggCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGTCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTg                                                                  <  3:268285/145‑1 (MQ=255)
                                                                                     gCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCa                                                                                                   >  3:168861/1‑110 (MQ=255)
                                                                                     gCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCa                                                                                                   <  4:168861/110‑1 (MQ=255)
                                                                                          cTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAggg                                                       <  8:271062/149‑1 (MQ=255)
                                                                                           tCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGTGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAGGGt                                                      >  6:214285/1‑149 (MQ=255)
                                                                                           tCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAAt                                                                                    <  4:34024/119‑1 (MQ=255)
                                                                                           tCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAAt                                                                                    >  3:34024/1‑119 (MQ=255)
                                                                                            ccAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAggg                                                       <  5:174621/147‑1 (MQ=255)
                                                                                            ccAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAggg                                                       >  6:174621/1‑147 (MQ=255)
                                                                                                  cgcgAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAGGGTTGCAtt                                                >  7:262004/1‑148 (MQ=255)
                                                                                                    cgAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAGGGTTGCATTAAt                                             <  4:38749/149‑1 (MQ=255)
                                                                                                    cgAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAGGGTTGCATTAAt                                             <  8:262004/149‑1 (MQ=255)
                                                                                                    cgAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAGGGTTGCATTAAt                                             <  2:157748/149‑1 (MQ=255)
                                                                                                           ggcgGTTGGCTGCGTCGAAGCCGTGGGCAATATTCTTTTGGCGCTCTTGCAGAGCAGTTATGACCGGAGGCCATACATAATTCATGCAGAAGAGTACAAAAATAAGGAAAGCAACTGATTGTCCAATCAGGGTTGc                                                   <  6:77452/135‑1 (MQ=255)
                                                                                                           cgcgGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAGGGTTGCATTAATGTTCa                                        <  7:87562/147‑1 (MQ=255)
                                                                                                           cgcgGTTGGCAGCGTACAAGCCGTCCGCAATCTACTTATGGCGCTCTTGCAGAGCAGTGATGACCGGAGCCCATACAAACTTCATCCAGAAGACTACAAAAAACAGGAAAGAAAAGTATTGGACAATCAGGCTTGc                                                   >  5:77452/1‑136 (MQ=255)
                                                                                                            gcgGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGTGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAGGGTTGCATTAATGTTCACGc                                     <  5:214285/149‑1 (MQ=255)
                                                                                                             cgGTTGGCAGCGTCCAAGCCGTCGGCGATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAGGGTTGCATTAATGTTCACGc                                     >  1:172181/1‑148 (MQ=255)
                                                                                                                          ccAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAACAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAGGGTTGAATTAATGATCACGCCAACACctcgctcg                       >  2:137205/1‑149 (MQ=255)
                                                                                                                            aaGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAGGGTTGCATTAATGTTCACGCCAACACCTCGCTCgg                      >  5:133406/1‑148 (MQ=255)
                                                                                                                                     gCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAGGGTTGCATTAAt                                             <  2:302109/116‑1 (MQ=255)
                                                                                                                                       aaTCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAGGGTTGCATTAATGTTCACGCCAACACCTCGCTCGGTCTTAAtt              <  8:136176/145‑1 (MQ=255)
                                                                                                                                       aaTCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAGGGTTGCATTAATGTTCACGCCAACACCTCGCTCGGTCTTAAtt              >  7:136176/1‑145 (MQ=255)
                                                                                                                                       aaTCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAGGGTTGCATTAATGTTCACGCCAACACCTCGCTCGGTCTTAATTCATa          >  2:214975/1‑149 (MQ=255)
                                                                                                                                       aaTCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAGGGTTGCATTAATGTTCACGCCAACACCTCGCTCGGTCTTAATTCATa          <  2:28533/149‑1 (MQ=255)
                                                                                                                                                ttGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAGGGTTGCATTAATGTTCACGCCAACACCTCGCTCGGTCTTAATTCATACAGCCATc  <  8:44285/148‑1 (MQ=255)
                                                                                                                                                ttGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAGGGTTGCATTAATGTTCACGCCAACACCTCGCTCGGTCTTAAATCATACAGCCATc  >  6:248667/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CTGAGCAGCGCGTTTCTTGCTTTGCTCGATGATTTCGGCTGCTTGTGCTTTAGCTTCACGCAGTTGCTGACCCGCTTTCTCTTGGGCCAGCTCCAGGTCGCGAGCTGCGCGGTTGGCAGCGTCCAAGCCGTCGGCAATCTTCTTTTGGCGCTCTTGCAGAGCAGTGATGACCGGAGGCCATACATACTTCATGCAGAAGAGTACAAAAATCAGGAAAGCAACGGATTGGCCAATCAGGGTTGCATTAATGTTCACGCCAACACCTCGCTCGGTCTTAATTCATACAGCCATC  >  NC_002947/6179864‑6180155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑