Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 548,803 A→C 9.5% E551D (GAA→GAC)  fusA → elongation factor G 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947548,8030AC 9.5% 115.0 / 4.1 42E551D (GAA→GAC) fusAelongation factor G 1
Reads supporting (aligned to +/- strand):  ref base A (21/17);  new base C (2/2);  total (23/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CCAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTC  >  NC_002947/548661‑548949
                                                                                                                                              |                                                                                                                                                  
ccAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTGTGGTGTTCTCGAACGAAGGCGtt                                                                                                                                              >  8:290170/1‑149 (MQ=255)
ccAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTc                                                                                                                                                 >  1:293186/1‑146 (MQ=255)
ccAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTc                                                                                                                                                 <  2:293186/146‑1 (MQ=255)
ccAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGtt                                                                                                                                              >  5:82848/1‑149 (MQ=255)
ccAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGGCTGGTGTTCTCCAACGAAGTCGtt                                                                                                                                              >  5:77138/1‑149 (MQ=255)
 cAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTtg                                                                                                                                             >  1:280884/1‑149 (MQ=255)
 cAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTtg                                                                                                                                             >  8:274828/1‑149 (MQ=255)
  aaaTCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTtgg                                                                                                                                            >  1:73663/1‑149 (MQ=255)
  aaaTCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTtgg                                                                                                                                            <  2:280884/149‑1 (MQ=255)
   aaTCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGACTTCGTtgga                                                                                                                                           <  7:42082/149‑2 (MQ=255)
        aaCGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGgtgt                                                                                                                                      <  7:274828/149‑1 (MQ=255)
         aCGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGtgtg                                                                                                                                     <  2:73663/149‑1 (MQ=255)
              gagaTCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTc                                                                                                                                 <  7:290170/148‑1 (MQ=255)
                  tCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGGTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAg                                                                                                                            <  6:77138/149‑1 (MQ=255)
                    gAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAgg                                                                                                                           >  8:131093/1‑148 (MQ=255)
                      aGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAAt                                                                                                                        <  6:82848/149‑1 (MQ=255)
                                gttcgtCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGAAAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGc                                                                                                              >  5:286435/1‑149 (MQ=255)
                                  tcgtCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAg                                                                                                                            >  7:288096/1‑133 (MQ=255)
                                  tcgtCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAg                                                                                                                            <  8:288096/133‑1 (MQ=255)
                                    gtCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATcc                                                                                                                  >  8:68200/1‑141 (MQ=255)
                                    gtCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATcc                                                                                                                  <  7:68200/141‑1 (MQ=255)
                                    gtCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATc                                                                                                          <  4:53263/149‑1 (MQ=255)
                                         tCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAg                                                                                                       >  7:184356/1‑147 (MQ=255)
                                                gtcgtggtcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATc                                                                                              <  7:131093/149‑1 (MQ=255)
                                                gtcgtggtcAGTTCGGTCACTGATGGGTTCGCTTATCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTAt                                                                                               <  7:221886/148‑1 (MQ=255)
                                                   gtggtcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGACTTCGTtgga                                                                                                                                           >  4:179091/1‑100 (MQ=255)
                                                   gtggtcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGACTTCGTtgga                                                                                                                                           <  3:179091/101‑2 (MQ=255)
                                                         aGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCa                                                                                      <  8:184356/148‑1 (MQ=255)
                                                                        gggTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCgtt                                                                      >  7:120711/1‑149 (MQ=255)
                                                                              gCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGcc                                                                >  3:275443/1‑149 (MQ=255)
                                                                                ttCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAATAGCAGATGAAAAACGGCGTTGTTGCCgg                                                              >  3:115666/1‑149 (MQ=255)
                                                                                ttCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCgg                                                              >  8:72488/1‑149 (MQ=255)
                                                                                     cGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATc                                                         >  1:2580/1‑149 (MQ=255)
                                                                                               gTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCgg                                               <  7:72488/149‑1 (MQ=255)
                                                                                                    cgacAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGa                                          <  8:120711/149‑1 (MQ=255)
                                                                                                              aaCATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGt                                <  2:2580/149‑1 (MQ=255)
                                                                                                                 aTCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTAtt                             <  3:16547/149‑1 (MQ=255)
                                                                                                                             cTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTa                 >  2:37731/1‑149 (MQ=255)
                                                                                                                                    ccaccAACGACTTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCacgac          >  8:136748/4‑149 (MQ=255)
                                                                                                                                        cAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTc       >  5:120878/1‑148 (MQ=255)
                                                                                                                                         aaCGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCg      >  1:208129/1‑148 (MQ=255)
                                                                                                                                            gAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTc  >  1:7391/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
CCAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTC  >  NC_002947/548661‑548949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑