Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 548,804 G→T 9.2% V552F (GTC→TTC)  fusA → elongation factor G 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947548,8040GT 9.2% 125.4 / 2.1 42V552F (GTC→TTC) fusAelongation factor G 1
Reads supporting (aligned to +/- strand):  ref base G (21/17);  new base T (2/2);  total (23/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAA  >  NC_002947/548661‑548952
                                                                                                                                               |                                                                                                                                                    
ccAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTGTGGTGTTCTCGAACGAAGGCGtt                                                                                                                                                 >  8:290170/1‑149 (MQ=255)
ccAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTc                                                                                                                                                    >  1:293186/1‑146 (MQ=255)
ccAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTc                                                                                                                                                    <  2:293186/146‑1 (MQ=255)
ccAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGtt                                                                                                                                                 >  5:82848/1‑149 (MQ=255)
ccAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGGCTGGTGTTCTCCAACGAAGTCGtt                                                                                                                                                 >  5:77138/1‑149 (MQ=255)
 cAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTtg                                                                                                                                                >  1:280884/1‑149 (MQ=255)
 cAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTtg                                                                                                                                                >  8:274828/1‑149 (MQ=255)
  aaaTCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTtgg                                                                                                                                               >  1:73663/1‑149 (MQ=255)
  aaaTCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTtgg                                                                                                                                               <  2:280884/149‑1 (MQ=255)
   aaTCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGACTTCGTtgga                                                                                                                                              <  7:42082/149‑2 (MQ=255)
        aaCGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGgtgt                                                                                                                                         <  7:274828/149‑1 (MQ=255)
         aCGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGtgtg                                                                                                                                        <  2:73663/149‑1 (MQ=255)
              gagaTCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTc                                                                                                                                    <  7:290170/148‑1 (MQ=255)
                  tCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGGTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAg                                                                                                                               <  6:77138/149‑1 (MQ=255)
                    gAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAgg                                                                                                                              >  8:131093/1‑148 (MQ=255)
                      aGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAAt                                                                                                                           <  6:82848/149‑1 (MQ=255)
                                gttcgtCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGAAAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGc                                                                                                                 >  5:286435/1‑149 (MQ=255)
                                  tcgtCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAg                                                                                                                               >  7:288096/1‑133 (MQ=255)
                                  tcgtCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAg                                                                                                                               <  8:288096/133‑1 (MQ=255)
                                    gtCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATcc                                                                                                                     >  8:68200/1‑141 (MQ=255)
                                    gtCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATcc                                                                                                                     <  7:68200/141‑1 (MQ=255)
                                    gtCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATc                                                                                                             <  4:53263/149‑1 (MQ=255)
                                         tCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAg                                                                                                          >  7:184356/1‑147 (MQ=255)
                                                gtcgtggtcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATc                                                                                                 <  7:131093/149‑1 (MQ=255)
                                                gtcgtggtcAGTTCGGTCACTGATGGGTTCGCTTATCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTAt                                                                                                  <  7:221886/148‑1 (MQ=255)
                                                   gtggtcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGACTTCGTtgga                                                                                                                                              >  4:179091/1‑100 (MQ=255)
                                                   gtggtcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGACTTCGTtgga                                                                                                                                              <  3:179091/101‑2 (MQ=255)
                                                         aGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCa                                                                                         <  8:184356/148‑1 (MQ=255)
                                                                        gggTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCgtt                                                                         >  7:120711/1‑149 (MQ=255)
                                                                              gCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGcc                                                                   >  3:275443/1‑149 (MQ=255)
                                                                                ttCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAATAGCAGATGAAAAACGGCGTTGTTGCCgg                                                                 >  3:115666/1‑149 (MQ=255)
                                                                                ttCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCgg                                                                 >  8:72488/1‑149 (MQ=255)
                                                                                     cGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATc                                                            >  1:2580/1‑149 (MQ=255)
                                                                                               gTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCgg                                                  <  7:72488/149‑1 (MQ=255)
                                                                                                    cgacAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGa                                             <  8:120711/149‑1 (MQ=255)
                                                                                                              aaCATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGt                                   <  2:2580/149‑1 (MQ=255)
                                                                                                                 aTCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTAtt                                <  3:16547/149‑1 (MQ=255)
                                                                                                                             cTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTa                    >  2:37731/1‑149 (MQ=255)
                                                                                                                                    ccaccAACGACTTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCacgac             >  8:136748/4‑149 (MQ=255)
                                                                                                                                        cAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTc          >  5:120878/1‑148 (MQ=255)
                                                                                                                                         aaCGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCg         >  1:208129/1‑148 (MQ=255)
                                                                                                                                            gAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTc     >  1:7391/1‑149 (MQ=255)
                                                                                                                                               gTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCaa  >  2:254802/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CCAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAA  >  NC_002947/548661‑548952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑