Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 559,579 G→T 8.7% G43V (GGT→GTT)  rplO → 50S ribosomal protein L15

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947559,5790GT 8.7% 170.9 / 4.4 57G43V (GGT→GTT) rplO50S ribosomal protein L15
Reads supporting (aligned to +/- strand):  ref base G (26/26);  new base T (2/3);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

GGCTAATCGATGAAACTCAATGATCTGAGTCCAGCGCCGGGTTCCCGTCGCGAGAAGCATCGTCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCTGTCCGAGCTGGCCAAAGTGGAAGGCGACGTGATCTCCGTG  >  NC_002947/559443‑559727
                                                                                                                                         |                                                                                                                                                      
ggCTAATCGATGAAACTCAATGATCTGAGTCCAGCGCCGGGTTCCCGTCGCGAGAAGCATCGTCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑ACGTCAGACTTCCCGTTCGGGTGGTTCGATCg                                                                                                                                             >  2:144663/1‑148 (MQ=255)
     aTCGATGAAACTCAATGATCTGAGTCCAGCGCCGGGTTCCCGTCGCGAGAAGCATCGTCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTc‑c                                                                                                                                        <  4:249072/147‑1 (MQ=255)
      tCGATGAAACTCAATGATCTGAGTCCAGCGCCGGGTTCCCGTCGCGAGAAGCATCGTCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTgg                                                                                                                                     >  7:228908/1‑149 (MQ=255)
                        cTGAGTCCAGCGCCGGGTTCCCGTCGCGAGAAGCATCGTCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACa                                                                                                                  >  8:57625/1‑149 (MQ=255)
                               cAGCGCCGGGTTCCCGTCGCGAGAAGCATCGTCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTg                                                                                                           >  5:277973/1‑149 (MQ=255)
                                 gcgcCGGGTTCCCGTCGCGAGAAGCATCGTCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCa                                                                                                         >  1:235954/1‑149 (MQ=255)
                                 gcgcCGGGTTCCCGTCGCGAGAAGCATCGTCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCa                                                                                                         <  3:138575/149‑1 (MQ=255)
                                  cgcCGGGTTCCCGTCGCGAGAAGCATCGTCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCAc                                                                                                        <  6:277973/149‑1 (MQ=255)
                                   gcCGGGTTCCCGTCGCGAGAAGCATCGTCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGACGCTGcccc                                                                                                       >  4:239009/1‑149 (MQ=255)
                                         ttCCCGTCGCGAGAAGCATCGTCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGcc                                                                                                 >  7:130899/1‑149 (MQ=255)
                                           cccGTCGCGAGAAGCATCGTCCAGGTCGTGGTATCGGTAGCGGTCTGTGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCt                                                                                                <  7:127750/148‑1 (MQ=255)
                                           cccGTCGCGAGAAGCATCGTCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCt                                                                                                <  6:119472/148‑1 (MQ=255)
                                                  cgAGAAGCATCGTCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAg                                                                                         <  8:228908/148‑1 (MQ=255)
                                                     gaAGCATCGTCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGATCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCg                                                                                     <  5:318938/149‑1 (MQ=255)
                                                       aGCATCGTCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGc                                                                                   <  8:130899/149‑1 (MQ=255)
                                                          aTCGTCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTc                                                                                <  2:235954/149‑1 (MQ=255)
                                                              tCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGcc                                                                                                               <  7:227247/114‑1 (MQ=255)
                                                              tCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGcc                                                                                                               >  8:227247/1‑114 (MQ=255)
                                                              tCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGttt                                                                            >  3:76348/1‑149 (MQ=255)
                                                                   gTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGGTCGGCTTCGTTTCCCt                                                                        >  2:220967/1‑148 (MQ=255)
                                                                    tCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGTTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTg                                                                       >  5:325034/1‑148 (MQ=255)
                                                                    tCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGTGGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTg                                                                       >  5:231375/1‑148 (MQ=255)
                                                                      gTGGTATCGGTAGCGGTCTGGTTAAGACCGGCGGCCGTGGCCAAAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGaaa                                                                    <  6:282665/149‑1 (MQ=255)
                                                                        ggTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGc                                                                  >  5:49692/1‑149 (MQ=255)
                                                                              ggTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATgg                                                             <  2:100642/148‑1 (MQ=255)
                                                                              ggTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGa                                                            >  1:110534/1‑149 (MQ=255)
                                                                                  gCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAAc                                                                                                                   <  7:280643/90‑1 (MQ=255)
                                                                                  gCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAAc                                                                                                                   >  8:280643/1‑90 (MQ=255)
                                                                                  gCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGCTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACcgc                                                        <  4:86892/149‑1 (MQ=255)
                                                                                   cGGTCTGGGTAAGACCGGCGGCCGTGGCCACAACAGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTACGTTTCCCTGAAAGCCATGGACcgc                                                        >  1:201045/1‑149 (MQ=255)
                                                                                    ggTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGTTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCgcgc                                                      <  6:325034/149‑1 (MQ=255)
                                                                                    ggTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGTTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCgcgc                                                      <  6:231375/149‑1 (MQ=255)
                                                                                    ggTCTGGGTAAGACCAGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGATCGATCACTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCgcgc                                                      <  8:199408/149‑1 (MQ=255)
                                                                                     gTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCt                                                     >  5:106492/1‑149 (MQ=255)
                                                                                     gTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCt                                                     <  2:201045/149‑1 (MQ=255)
                                                                                          ggTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGaa                                                  <  6:130550/147‑1 (MQ=255)
                                                                                           gTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTCTGTGGCTACGAAGGC‑GGTCAACAGCCGGTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCGTGAAAGCCATGGACCGCGCTGAAGt                                                >  1:92312/1‑149 (MQ=255)
                                                                                            tAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGc                                              >  5:329411/1‑149 (MQ=255)
                                                                                                aCCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCt                                          >  5:156389/1‑149 (MQ=255)
                                                                                                  cggcggCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGTTGGTTCGATCGCTC‑CTTGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGAcc                                                          <  1:323799/131‑1 (MQ=255)
                                                                                                  cggcggCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGAcc                                                          >  2:323799/1‑131 (MQ=255)
                                                                                                  cggcggCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCTGt                                        <  7:57625/149‑1 (MQ=255)
                                                                                                    gcggcCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCTGTcc                                      >  8:236404/1‑149 (MQ=255)
                                                                                                        ccGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACAGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCTGTCCGAGc                                  >  7:20398/1‑149 (MQ=255)
                                                                                                          gTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGTTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCTGTCCGAGCTg                                <  3:286121/149‑1 (MQ=255)
                                                                                                             gCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCTGTCCGAGCTGGc                              <  6:49692/148‑1 (MQ=255)
                                                                                                              ccACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCTGTCCGAGCTGGCCa                            <  4:76348/149‑1 (MQ=255)
                                                                                                               cacaA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGCGGGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCTGTCCGAGCTGGcc                             >  1:245623/1‑148 (MQ=255)
                                                                                                                   a‑aGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCTGTCCGAGCTGGCCAAAGTg                       <  2:92312/149‑1 (MQ=255)
                                                                                                                        tCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCt                                          >  2:47649/1‑126 (MQ=255)
                                                                                                                        tCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCt                                          <  1:47649/126‑1 (MQ=255)
                                                                                                                        tCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCTGTCCGAGCTGGCCAAAGTGGAAg                   >  8:89024/1‑149 (MQ=255)
                                                                                                                        tCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCTGTCCGAGCTGGCCAAAGTGGAAg                   <  6:106492/149‑1 (MQ=255)
                                                                                                                               tCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCTGTCCGAGCTGGCCAAAGTGGAAGGCGACGt            >  7:305183/1‑149 (MQ=255)
                                                                                                                                cccGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCTGTCCGAACTGGCCAAAGTGGAAGGCGACg             <  2:110534/147‑1 (MQ=255)
                                                                                                                                 ccGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCTGTCCGAGCTGGCGAAAGTGGAAGGCGACGTGa          >  3:182510/1‑149 (MQ=255)
                                                                                                                                  cGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCTGTCCGAGCTGGCCAAAGTGGAAGGCGACGTGAt         <  1:144663/149‑1 (MQ=255)
                                                                                                                                         gtggtTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCTGTCCGAGCTGGCCAAAGTGGAAGGCGACGTGATCTCCGTg  <  7:236404/149‑1 (MQ=255)
                                                                                                                                         gtggtTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCTGTCCGAGCTGGCCAAAGTGGAAGGCGACGTGATCTCCGTg  <  6:156389/149‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                      
GGCTAATCGATGAAACTCAATGATCTGAGTCCAGCGCCGGGTTCCCGTCGCGAGAAGCATCGTCCAGGTCGTGGTATCGGTAGCGGTCTGGGTAAGACCGGCGGCCGTGGCCACAA‑AGGTCAGACTTCCCGTTCGGGTGGTTCGATCGCTC‑CTGGCTTCGAAGGC‑GGTCAACAGCCGCTGCACCGTCGCCTGCCGAAGTTCGGCTTCGTTTCCCTGAAAGCCATGGACCGCGCTGAAGTGCGTCTGTCCGAGCTGGCCAAAGTGGAAGGCGACGTGATCTCCGTG  >  NC_002947/559443‑559727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑