Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	584,235	T→A	8.8%	intergenic (+76/+31)	PP_t75 → / ← ansA	tRNA‑Sec/type 1 L‑asparaginase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	584,235	0	T	A	8.8%	180.3 / 4.4	64	intergenic (+76/+31)	PP_t75/ansA	tRNA‑Sec/type 1 L‑asparaginase
Reads supporting (aligned to +/- strand):  ref base T (29/29);  new base A (3/3);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGGGCTTCAAACCCGGTTGGGGACGGCAGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGGCTGCTTCACGGGTCAT  >  NC_002947/584090‑584376                                                                                                                                                  |                                                                                                                                              gCCGGGCTTCAAACCCGGTTGGGGACGGCAGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTAc                                                                                                                                            >  7:64267/1‑149 (MQ=255) gCCGGGCTTCAAACCCGGTTGGGGACGGCAGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTAc                                                                                                                                            <  3:197031/149‑1 (MQ=255)        ttCAAACCCGGTTGGGGACGGCAGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCcgcg                                                                                                                                       >  5:252884/1‑147 (MQ=255)        tgcAACCCCGGTGGGGGACGCCTCCCG‑TCCCCGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTAAACCcgcgcct                                                                                                                                    <  3:127618/147‑4 (MQ=255)                cGGTTGGGGACGGCAGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACACGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCt                                                                                                                             >  3:305092/1‑149 (MQ=255)                  gTTGGGGACGGCAGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTc                                                                                                                            >  8:37325/1‑148 (MQ=255)                       ggACGGCAGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGgc                                                                                                                      >  7:128244/1‑149 (MQ=255)                         aCGGCAGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGgcgc                                                                                                                    <  4:238250/149‑1 (MQ=255)                              aGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGaa                                                                                                                                     >  4:209917/1‑127 (MQ=255)                              aGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGaa                                                                                                                                     <  3:209917/127‑1 (MQ=255)                              aGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCt                                                                                                                   <  8:93826/145‑1 (MQ=255)                              aGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCt                                                                                                                   >  7:93826/1‑145 (MQ=255)                                ccGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTTGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCaa                                                                                                             <  1:262816/149‑1 (MQ=255)                                ccGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCaa                                                                                                             <  4:95270/149‑1 (MQ=255)                                ccGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCaa                                                                                                             <  6:252884/149‑1 (MQ=255)                                  gTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGc                                                                                                           <  7:37325/149‑1 (MQ=255)                                                tCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCcacac                                                                                              >  1:196633/1‑148 (MQ=255)                                                   aCTCCCGCTGCCTTCCGCCATTTTTTCACACTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCAACACAGGAAACGCAAGCGTCCTAGGCGCGGGTTTACCCCCGAAGAGGCCCTCAACGGCCCTGATCAAGCCCGCTCGCCACACAAAt                                                                                          >  2:248115/1‑149 (MQ=255)                                                       ccGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAgg                                                                                                                                 <  3:51081/106‑1 (MQ=255)                                                       ccGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAgg                                                                                                                                 >  4:51081/1‑106 (MQ=255)                                                       ccGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGc                                                                                                   >  6:39925/1‑136 (MQ=255)                                                       ccGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGc                                                                                                   <  5:39925/136‑1 (MQ=255)                                                       ccGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAg                                                                                      >  3:247850/1‑149 (MQ=255)                                                         gCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGa                                                                                    >  2:33027/1‑149 (MQ=255)                                                                 ccGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGaa                                                                                                                                     >  2:299702/1‑92 (MQ=255)                                                                 ccGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGaa                                                                                                                                     <  1:299702/92‑1 (MQ=255)                                                                  cGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTAAACCcgcgcct                                                                                                                                    <  1:195165/92‑4 (MQ=255)                                                                  cGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTAAACCcgcgcct                                                                                                                                    >  2:195165/1‑89 (MQ=255)                                                                   gCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGgcg                                                                                                                     <  2:5366/106‑1 (MQ=255)                                                                   gCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGgcg                                                                                                                     >  1:5366/1‑106 (MQ=255)                                                                   gCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGc                                                                           >  7:200137/1‑148 (MQ=255)                                                                   gCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGc                                                                           <  8:200137/148‑1 (MQ=255)                                                                   gCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCg                                                                          <  8:128244/149‑1 (MQ=255)                                                                   gCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCg                                                                          <  8:64267/149‑1 (MQ=255)                                                                     cATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGt                                                                        >  6:100465/1‑149 (MQ=255)                                                                         ttttCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTctgc                                                                    >  8:30261/1‑149 (MQ=255)                                                                            tCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCtgctgc                                                                 >  1:136095/1‑149 (MQ=255)                                                                               cccTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAAGCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTc                                                              <  5:273529/149‑1 (MQ=255)                                                                                         gCTCTTCGCGGTTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAAc                                                    <  4:305092/149‑1 (MQ=255)                                                                                           tctTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAAccc                                                  >  8:24113/1‑149 (MQ=255)                                                                                           tctTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAAccc                                                  >  6:207958/1‑149 (MQ=255)                                                                                             ttCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTg                                                >  4:202821/1‑149 (MQ=255)                                                                                               cgcgGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTgc                                               <  4:247850/148‑1 (MQ=255)                                                                                                  gggTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGccc                                           <  1:234592/149‑1 (MQ=255)                                                                                                       aaCCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAg                                      >  6:22530/1‑149 (MQ=255)                                                                                                        aCCCGCTCCTCCACCCGAAACGCAAGCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCTAGGGCGCTGATCAAGCCCGCTCGCCACAAAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAgc                                     <  8:284104/149‑1 (MQ=255)                                                                                                        aCCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAgc                                     <  5:100465/149‑1 (MQ=255)                                                                                                          ccGCTCCTACCCAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAgcgc                                   <  8:161767/149‑1 (MQ=255)                                                                                                                cTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGccacc                               <  7:30261/147‑1 (MQ=255)                                                                                                                    acaGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTg                         >  2:208585/1‑149 (MQ=255)                                                                                                                     caGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCt                           <  3:251670/146‑1 (MQ=255)                                                                                                                     caGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCt                           >  4:251670/1‑146 (MQ=255)                                                                                                                           aCGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAggg                  <  5:207958/149‑1 (MQ=255)                                                                                                                           aCGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGGAGCGCCACCATCTTGCCCCggg                  >  7:49567/1‑149 (MQ=255)                                                                                                                                  cgtcgtAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCACCCAACCCTGCGCCCAGTAgcg                                    <  4:79357/124‑1 (MQ=255)                                                                                                                                  cgtcgtAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCAACCAACCCTGCGCCCAGTAgcg                                    >  3:79357/1‑124 (MQ=255)                                                                                                                                      gtAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGGCTGCTTCACgg       >  8:92261/1‑149 (MQ=255)                                                                                                                                      ctcttcgcgGGTAAACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGgctgct             >  8:106858/6‑143 (MQ=255)                                                                                                                                      ctcttcgcgGGTAAACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGgctgct             <  7:106858/138‑1 (MQ=255)                                                                                                                                         ttcgcgGGTAAACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGGCTGCTTCACGGGt     >  2:155129/3‑148 (MQ=255)                                                                                                                                         ggCGCGGGTTTACCCGCGAAGAGTCCCTCAAGGGCGCTGATCATGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCTGTAGCGCCACCATCTTGCCCAGGGCTGCTTCACGGGTc    <  1:248115/149‑1 (MQ=255)                                                                                                                                         ggCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGGCTGCTTCACGGGTc    <  2:196633/149‑1 (MQ=255)                                                                                                                                          gcgcGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCCCACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGGCTGCTTCACGGGTCa   >  8:233102/1‑149 (MQ=255)                                                                                                                                           cgcgGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGGCTGCTTCACGGGTCAt  <  5:22530/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                              GCCGGGCTTCAAACCCGGTTGGGGACGGCAGCCGTTCCCGGGCAGGTTCGACTCCCGCTGCCTTCCGCCATTTTTTCACCCTTTAGGGGCTCTTCGCGGGTGAACCCGCTCCTACACAGGAAACGCAATCGTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTCAAGGGCGCTGATCAAGCCCGCTCGCCACACAAATCCAGGACAAACCAGCGGTCTGCTGCGTCAGCATCCAACCCTGCGCCCAGTAGCGCCACCATCTTGCCCAGGGCTGCTTCACGGGTCAT  >  NC_002947/584090‑584376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑