Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 591,703 C→G 14.2% intergenic (+17/+14) PP_0501 → / ← PP_0502 NAD‑dependent epimerase/dehydratase family protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947591,7030CG14.2% 123.4 / 12.2 49intergenic (+17/+14)PP_0501/PP_0502NAD‑dependent epimerase/dehydratase family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (19/23);  new base G (4/3);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.76e-01

CTATGCGGCGGCGCGATCGGGTGATATCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTCCACCAGTTCCGAACGCGCTTCTCCCGC  >  NC_002947/591557‑591848
                                                                                                                                                  |                                                                                                                                                 
cTATGCGGCGGCGCGATCGGGTGATATCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTcag                                                                                                                                                 >  7:6383/1‑149 (MQ=255)
      ggcggcgCGATCGGGTGATATCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGccc                                                                                                                                           >  2:302306/1‑149 (MQ=255)
       gcggcgCGATCGGGTGATATCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGCTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTGAGCAGcccc                                                                                                                                          <  2:147174/149‑2 (MQ=255)
          gcgcGATCGGGTGATATCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGAc                                                                                                                                       <  8:6383/149‑1 (MQ=255)
           cgcgATCGGGTGATATCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCAGGGCTGCTGAGCAGccccgcgg                                                                                                                                      <  7:192691/149‑6 (MQ=255)
                  gggTTATATCCGCCATTCGCGTGCTGATAACCCGCGGTTTTTTGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCa                                                                                                                               <  1:108156/149‑1 (MQ=255)
                  gggTGATATCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCAccc                                                                                                                                <  2:6580/148‑1 (MQ=255)
                  gggTGATATCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCa                                                                                                                               >  8:77178/1‑149 (MQ=255)
                       atatCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCg                                                                                                                          <  8:247449/149‑1 (MQ=255)
                          tCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTAGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTGAGCAGcccc                                                                                                                                          <  8:261886/130‑2 (MQ=255)
                          tCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTAGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTGAGCAGcccc                                                                                                                                          >  7:261886/1‑129 (MQ=255)
                           ccGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGc                                                                                                                         <  8:97291/146‑1 (MQ=255)
                           ccGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGc                                                                                                                         >  7:97291/1‑146 (MQ=255)
                                    gcgAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTgcgc                                                                                                             <  4:72559/149‑1 (MQ=255)
                                     cgAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTGGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCt                                                                                                            <  2:201203/149‑1 (MQ=255)
                                                   gCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGa                                                                                              >  8:170947/1‑149 (MQ=255)
                                                     ggCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCCCCCTTCTGCGCTGGCAATCTTCGGGagg                                                                                            >  8:257021/1‑147 (MQ=255)
                                                             gcgcGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGAccc                                                                                    >  8:60601/1‑149 (MQ=255)
                                                                         tttGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGc                                                                        <  4:239331/149‑1 (MQ=255)
                                                                            gcgcAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCa                                                                      <  1:302306/148‑1 (MQ=255)
                                                                                  gTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGCGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGAGGTAAGCCCCGCCCATCCTGGCCCCGGACCt                                                               >  8:216906/1‑149 (MQ=255)
                                                                                  gTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCt                                                               >  8:46383/1‑149 (MQ=255)
                                                                                     aCGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCCGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGc                                                             <  7:101219/148‑1 (MQ=255)
                                                                                       gCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTc                                                                                                  >  6:284383/1‑109 (MQ=255)
                                                                                       gCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTc                                                                                                  <  5:284383/109‑1 (MQ=255)
                                                                                          gATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATAtt                                                       >  3:188546/1‑149 (MQ=255)
                                                                                               ttGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAg                                                  >  6:23072/1‑149 (MQ=255)
                                                                                                 gAATGCCTGACTAAGATTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCTCCCATCCTGGCCACGGACCTGCAATATTTCAAgc                                                 <  6:23283/148‑1 (MQ=255)
                                                                                                      ccTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGc                                           <  7:170947/149‑1 (MQ=255)
                                                                                                               gCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACg                                  >  4:231372/1‑149 (MQ=255)
                                                                                                                cTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGc                                 <  7:46383/149‑1 (MQ=255)
                                                                                                                 ttCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGcc                                >  5:42192/1‑149 (MQ=255)
                                                                                                                   ctcGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGcc                                <  6:42192/147‑1 (MQ=255)
                                                                                                                   ctcGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCg                               <  3:231372/148‑1 (MQ=255)
                                                                                                                      ggTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATAtt                                                       >  1:247805/1‑121 (MQ=255)
                                                                                                                      ggTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATAtt                                                       <  2:247805/121‑1 (MQ=255)
                                                                                                                      ggTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTcc                            <  7:77178/148‑1 (MQ=255)
                                                                                                                      ggTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATAATTCAAGCGCCTGCCCCGTCACGCCGTcca                           <  6:96046/149‑1 (MQ=255)
                                                                                                                         aaaGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTccac                          >  4:143263/1‑147 (MQ=255)
                                                                                                                           aGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTCCACCAGt                      >  5:220784/1‑149 (MQ=255)
                                                                                                                           aGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTCCACCAGt                      >  3:208762/1‑149 (MQ=255)
                                                                                                                              agcaGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTCCACCAGTTcc                   >  6:316343/4‑149 (MQ=255)
                                                                                                                                gAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCCCCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTCCCCCAGTTCCg                  >  3:240723/1‑148 (MQ=255)
                                                                                                                                 aGCCGCGCGGGGCTGCTCAGCAGCCCGGACATCACGCATGCCGCACCGTTCTTCGCTGGCTATCTTACGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCATTATTTCAAGCGCCTGCCCCGTCACGCCGTCCACCAGTTCCg                  <  7:270504/147‑1 (MQ=255)
                                                                                                                                         agggCTGCTGAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCaa                                                   >  6:40846/2‑106 (MQ=255)
                                                                                                                                         agggCTGCTGAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCaa                                                   >  6:36067/2‑106 (MQ=255)
                                                                                                                                         agggCTGCTGAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTCCACCAGTTCCGAACGCGCTtc        >  8:302662/2‑149 (MQ=255)
                                                                                                                                          gggCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTCCACCAGTTCCGAACGCGCTtct       <  7:216906/149‑1 (MQ=255)
                                                                                                                                                ctCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTCCACCAGTTCCGAACGCGCTTCTCCCGc  <  6:220784/148‑1 (MQ=255)
                                                                                                                                                ctCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTCCACCAGTTCCGAACGCGCTTCTCCCGc  <  5:23072/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTATGCGGCGGCGCGATCGGGTGATATCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTCCACCAGTTCCGAACGCGCTTCTCCCGC  >  NC_002947/591557‑591848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑