Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 603,648 T→G 8.7% intergenic (+44/‑48) ribAB‑I → / → ribH bifunctional 3,4‑dihydroxy‑2‑butanone 4‑phosphate synthase/GTP cyclohydrolase II/6,7‑dimethyl‑8‑ribityllumazine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947603,6480TG 8.7% 131.7 / 4.1 46intergenic (+44/‑48)ribAB‑I/ribHbifunctional 3,4‑dihydroxy‑2‑butanone 4‑phosphate synthase/GTP cyclohydrolase II/6,7‑dimethyl‑8‑ribityllumazine synthase
Reads supporting (aligned to +/- strand):  ref base T (19/23);  new base G (2/2);  total (21/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

GTGACCTCGGTGTGCGCAAGATGCGCCTGATGAGTTCGCCGATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTCAACAGCTTCGTTGTCGAAAGCCTGG  >  NC_002947/603501‑603792
                                                                                                                                                   |                                                                                                                                                
gTGACCTCGGTGTGCGCAAGATGCGCCTGATGAGTTCGCCGATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTc                                                                                                                                                 <  1:61087/149‑1 (MQ=255)
     cTCGGTGTGCGCAAGATGCGCCTGATGAGTTCGCCGATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGc                                                                                                                                            >  5:330115/1‑149 (MQ=255)
       cGGTGTGCGCAAGATGCGCCTGATGAGTTCGCCGATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGccc                                                                                                                                          >  7:271467/1‑148 (MQ=255)
            tgCGCAAGATGCGCCTGATGAGTTCGCCGATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCGAGACACCTGTCGACGCCGCTTGt                                                                                                                                     >  4:186099/1‑149 (MQ=255)
            tgCGCAAGATGCGCCTGATGAGTTCGCCGATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTACTCGAGTCCAGACCCCTGTCGAGGCCGCTTGt                                                                                                                                     <  6:181451/149‑1 (MQ=255)
                        gcCTGATGAGTTCGCCGATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCATGTATGAGGCCTccc                                                                                                                         >  4:197104/1‑147 (MQ=255)
                            gatgaGTTCGCCGATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGTTTGTATGCGGCCTCGCTCtt                                                                                                                     >  6:280077/1‑149 (MQ=255)
                                        gATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAat                                                                                                         <  7:137834/149‑1 (MQ=255)
                                              gTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGa                                                                                                   <  8:288262/149‑1 (MQ=255)
                                                tCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGGCGAGGCCGCATACAAGCGGCCTCgacag                                                                                                                      >  5:8618/1‑124 (MQ=255)
                                                tCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGGCGAGGCCGCATACAAGCGGCCTCgacag                                                                                                                      <  6:8618/128‑5 (MQ=255)
                                                   aCGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGTTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGAc                                                                                              <  5:280077/149‑1 (MQ=255)
                                                       gATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTTGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCt                                                                                           >  2:5485/1‑148 (MQ=255)
                                                       gATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCATCCGAGTGACTTGTGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTg                                                                                          <  3:186099/149‑1 (MQ=255)
                                                                   cGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCt                                                                                                                            <  4:179545/103‑1 (MQ=255)
                                                                   cGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCt                                                                                                                            >  3:179545/1‑103 (MQ=255)
                                                                    gATCTGGAAGTTGTAGAATACGTGCCCTCCGATTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGaa                                                                              <  8:271467/148‑1 (MQ=255)
                                                                    gATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGCCCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAAAATCGGAATGACCCGGAAGACCCTCGAAg                                                                             >  6:243540/1‑149 (MQ=255)
                                                                    gATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCg                                                                                >  6:61338/1‑146 (MQ=255)
                                                                    gATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGaa                                                                              <  5:61338/148‑1 (MQ=255)
                                                                    gATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGaa                                                                              <  5:205058/148‑1 (MQ=255)
                                                                                gTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTGGTATGCGGCCTCGCTGTTGAAGATGAGAAAATCGGAATCAACCTGAAGACCCTCGAAGGTACCTTCATCg                                                                 >  5:231133/1‑149 (MQ=255)
                                                                                gTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCGTGTATTCGGCCGAGCTCTTGAAGATGAGAATATAGGAAATACCCTGAAGACCCTCGAAGGTACcatcatag                                                                 >  2:87851/1‑147 (MQ=255)
                                                                                 tAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCAt                                                                                  <  5:126951/131‑1 (MQ=255)
                                                                                  aGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGc                                                                <  3:197104/148‑1 (MQ=255)
                                                                                              cTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCt                                                                                           <  1:206963/109‑1 (MQ=255)
                                                                                              cTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCt                                                                                           >  2:206963/1‑109 (MQ=255)
                                                                                               tCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGccccc                                                            >  3:272558/1‑139 (MQ=255)
                                                                                               tCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGccccc                                                            <  4:272558/139‑1 (MQ=255)
                                                                                                     tGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGGCGAGGCCGCATACAAGCGGCCTCg                                                                                                                          <  2:189284/71‑1 (MQ=37)
                                                                                                     tGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGGCGAGGCCGCATACAAGCGGCCTCg                                                                                                                          >  1:189284/1‑71 (MQ=37)
                                                                                                        cTTGAGGCGGCATTGGCGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTtgg                                         <  5:236668/149‑1 (MQ=255)
                                                                                                        cTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTtgg                                         <  3:145872/149‑1 (MQ=255)
                                                                                                            aGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTtggtgg                                      <  4:42780/148‑1 (MQ=255)
                                                                                                                 gcATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGGGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGCCCATCGAACGGACCTTCATCCCCCCCCAACGTCGCGATGCTTTGGTTGTTGGcc                                >  6:60326/1‑149 (MQ=255)
                                                                                                                             tctGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTgg                                  <  3:203453/135‑1 (MQ=255)
                                                                                                                             tctGCTCGAGTCCAGACCCCTGTCGAGGCCGCGTGTATGCGGCCTCGCTCTTGAAGATGAGAATATAGGAATGACCCTGAAGACCATAGAAGGTACCTTCAAAGCCCCCAAAGGTCGCGATGCTTTGGTGGTTgg                                  >  4:203453/1‑135 (MQ=255)
                                                                                                                                 cTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTCAACAGCtt                   <  5:60326/146‑1 (MQ=255)
                                                                                                                                  tCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCGCTTGAAGATGAGAATATAGGAATGAACCAGAAGAACATCGAAGGTACCTTAATAGCCCCCAAAGGTAGCTATGCGTTTGTGGTTGGCCGCTTCAACAGCTTCGtt               >  4:78717/1‑149 (MQ=255)
                                                                                                                                  tCGAGGCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCGCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGGCGCTATGCTTTGGTGGTTGGCCGCTTCAACAGCTTCGtt               <  5:243540/149‑1 (MQ=255)
                                                                                                                                   cGAGTCCAGACCCCTGTCGAGGCCGCTTGTATTCGGCCTAGCTCTTGAAGATTAGAATATAGGAATTACCCTGAAGACCATCGAAGGTACCTTAATAGCCCCCAAAGGTCg                                                    >  6:303012/1‑111 (MQ=255)
                                                                                                                                   cGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCg                                                    <  5:303012/111‑1 (MQ=255)
                                                                                                                                    gAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTCAACAGCTTCGtt               <  3:157015/147‑1 (MQ=255)
                                                                                                                                              cccTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGc                              <  5:201110/122‑1 (MQ=255)
                                                                                                                                               ccTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGCCCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTCAACAGCTTCGTTGTCGAAAGCCTgg  >  7:234024/1‑149 (MQ=255)
                                                                                                                                                cTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTCAACAGCTTCGTTGTCGAAAGCCTgg  >  7:1345/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GTGACCTCGGTGTGCGCAAGATGCGCCTGATGAGTTCGCCGATGAAGTTCAACGCGATATCCGGATTCGATCTGGAAGTTGTAGAATACGTGCCCTCCGAGTGACTTGAGGCGGCATTGACGCCCTCTGCTCGAGTCCAGACCCCTGTCGAGGCCGCTTGTATGCGGCCTCGCTCTTGAAGATGAGAATATCGGAATGACCCTGAAGACCATCGAAGGTACCTTCATCGCCCCCAAAGGTCGCTATGCTTTGGTGGTTGGCCGCTTCAACAGCTTCGTTGTCGAAAGCCTGG  >  NC_002947/603501‑603792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑