Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 100,113 C→A 6.8% G99V (GGG→GTG)  PP_0094 ← 5'‑nucleotidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947100,1130CA 6.8% 224.8 / 4.1 72G99V (GGG→GTG) PP_00945'‑nucleotidase
Reads supporting (aligned to +/- strand):  ref base C (34/33);  new base A (3/2);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.04e-01

GCGAATCAGCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTG  >  NC_002947/99965‑100255
                                                                                                                                                    |                                                                                                                                              
gCGAATCAGCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGcc                                                                                                                                                >  6:123270/1‑149 (MQ=255)
        gCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGTCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAgg                                                                                                                                        >  5:272435/1‑149 (MQ=255)
        gCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAgg                                                                                                                                        <  8:7619/149‑1 (MQ=255)
        gCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGACGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCAGTTCAgg                                                                                                                                        >  6:225970/1‑149 (MQ=255)
            gACCTTGTTGGTGAGCGTTCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGTTGATGTAACGGAAAGTGCCGATCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGAGGCCCTGCACGTTCAGGGcc                                                                                                                                     <  7:221459/148‑1 (MQ=255)
              ccTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTTCCCGTTCAGGGCATc                                                                                                                                   >  3:225387/1‑148 (MQ=255)
               cTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCaa                                                                                                                                 >  6:81878/1‑149 (MQ=255)
                  gttgGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAg                                                                                                                                <  2:58404/147‑1 (MQ=255)
                  gttgGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAg                                                                                                                                <  7:25936/147‑1 (MQ=255)
                  gttgGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGc                                                                                                                               <  6:314283/148‑1 (MQ=255)
                      gTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGGAACTc                                                                                                                          >  1:219186/1‑149 (MQ=255)
                              gCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCacac                                                                                                                  >  2:6127/1‑149 (MQ=255)
                               ccGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGTGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACAcc                                                                                                                 >  4:49278/1‑149 (MQ=255)
                               ccGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCacac                                                                                                                  <  1:207545/148‑1 (MQ=255)
                                    cAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCg                                                                                                             <  5:123270/148‑1 (MQ=255)
                                          aCTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGTGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACg                                                                                                       <  3:49278/148‑1 (MQ=255)
                                              ccGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGtt                                                                                                  >  1:200395/1‑149 (MQ=255)
                                              ccGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGtt                                                                                                  >  8:245050/1‑149 (MQ=255)
                                               cGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGACGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCacac                                                                                                                  <  2:57977/132‑1 (MQ=255)
                                               cGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGACGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCacac                                                                                                                  >  1:57977/1‑132 (MQ=255)
                                                    aTCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGTCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGt                                                                                             <  5:15489/148‑1 (MQ=255)
                                                    aTCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGc                                                                                                                                      <  6:244811/107‑1 (MQ=255)
                                                       aCCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTaaa                                                                                          <  1:6127/148‑1 (MQ=255)
                                                         cTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGAc                                                                                       >  8:230916/1‑149 (MQ=255)
                                                                tgatgaTCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCAc                                                                                >  5:3645/1‑149 (MQ=255)
                                                                 gatgatCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTc                                                                                               >  6:282916/1‑133 (MQ=255)
                                                                 gatgatCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGAcc                                                                                      >  6:324126/1‑142 (MQ=255)
                                                                 gatgatCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGAcc                                                                                      <  5:324126/142‑1 (MQ=255)
                                                                      tCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCgg                                                                            >  2:219899/1‑147 (MQ=255)
                                                                        gAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGc                                                                                                                                      >  7:78448/1‑87 (MQ=255)
                                                                        gAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGc                                                                                                                                      <  8:78448/87‑1 (MQ=255)
                                                                        gAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAgc                                                                        >  3:79202/1‑149 (MQ=255)
                                                                               aaGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTcgcg                                                                 <  5:225970/149‑1 (MQ=255)
                                                                                     cGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGaa                                                           >  4:186923/1‑149 (MQ=255)
                                                                                     cGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGaa                                                           <  6:263863/149‑1 (MQ=255)
                                                                                      gAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGcac                                                                                                                    >  2:260592/1‑91 (MQ=255)
                                                                                      gAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGcac                                                                                                                    <  1:260592/91‑1 (MQ=255)
                                                                                      gAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAg                                                          >  2:232143/1‑149 (MQ=255)
                                                                                            tttCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTcgc                                                                  <  8:110279/135‑1 (MQ=255)
                                                                                            tttCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTcgc                                                                  >  7:110279/1‑135 (MQ=255)
                                                                                            tttCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCAcc                                                    <  3:186923/149‑1 (MQ=255)
                                                                                                   ggCGAACTCCCATGGTTTGGAGCTGGCAAAGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTACCAACACGACGTTATCGTAAAGACCGGTCACGCGGACGCGCTCGCGGTAGACTTGAACCGCATcc                                              >  4:161029/1‑148 (MQ=255)
                                                                                                       aaCTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGc                                         <  5:81878/149‑1 (MQ=255)
                                                                                                          tCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCCGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCtt                                      >  7:197153/1‑149 (MQ=255)
                                                                                                            ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTgg                                    >  8:102309/1‑149 (MQ=255)
                                                                                                            ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGAGGAAGCGCTCGCGGTACAAGTTCACCGCATCCCAGGCCTTgg                                    >  8:183064/1‑149 (MQ=255)
                                                                                                             cATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCaa                                                                                                                                 >  3:221724/1‑55 (MQ=255)
                                                                                                             cATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCaa                                                                                                                                 <  4:221724/55‑1 (MQ=255)
                                                                                                             cATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGc                                   <  2:240037/149‑1 (MQ=255)
                                                                                                              aTGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCt                                  >  5:17707/1‑149 (MQ=255)
                                                                                                                  tttGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgt                              <  2:200395/149‑1 (MQ=255)
                                                                                                                    tGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgtc                             <  7:102309/148‑1 (MQ=255)
                                                                                                                      gcggTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACGCGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgtcg                            >  5:74438/3‑147 (MQ=255)
                                                                                                                      gAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgtcg                            >  3:180306/1‑147 (MQ=255)
                                                                                                                      gAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGaa                          <  4:79202/149‑1 (MQ=255)
                                                                                                                       aGGTTGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGAGCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCAGCGCATCCCAGGCCTTGGCTTCGTCGAAg                         <  3:161029/149‑1 (MQ=255)
                                                                                                                       aGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAg                         >  5:1523/1‑149 (MQ=255)
                                                                                                                           ggCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATccc                                             <  8:107979/125‑1 (MQ=255)
                                                                                                                           ggCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGAATccc                                             >  7:107979/1‑125 (MQ=255)
                                                                                                                            gCAATGTACAGCGTGCGGCGCTGGCCGTTCAGGGCCTCAAGCAACTCCTGCTCAACTTCGATCACGAGGTTTTCGTAAAGTCCGGTCACGCGGATGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTa                    <  4:225387/149‑1 (MQ=255)
                                                                                                                            gCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTa                    <  1:219899/149‑1 (MQ=255)
                                                                                                                             cAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGaa                          <  4:36167/142‑1 (MQ=255)
                                                                                                                             cAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGaa                          >  3:36167/1‑142 (MQ=255)
                                                                                                                               aTGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGaa                 <  4:174868/149‑1 (MQ=255)
                                                                                                                                    cAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACGCGAGGTTTTCGTAAAGACCTGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGc             <  6:74438/148‑1 (MQ=255)
                                                                                                                                     tgcttgaggCCCTGAACGGGCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCAt           >  1:308012/8‑149 (MQ=255)
                                                                                                                                      gCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCAt                                                <  7:224837/111‑1 (MQ=255)
                                                                                                                                      gCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCAt                                                >  8:224837/1‑111 (MQ=255)
                                                                                                                                        ttgaggCCCTGAACGGGCAGGGCCGCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGa         >  5:177739/5‑148 (MQ=255)
                                                                                                                                         tgaggCCCTGAACGGGCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGt                                                               >  2:278583/4‑93 (MQ=255)
                                                                                                                                         tgaggCCCTGAACGGGCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGt                                                               <  1:278583/90‑1 (MQ=255)
                                                                                                                                         tGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGCCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGaaa       <  7:230916/149‑1 (MQ=255)
                                                                                                                                              cccTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTg  >  3:92661/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
GCGAATCAGCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTG  >  NC_002947/99965‑100255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑