Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 668,092 A→T 8.7% intergenic (+56/+96) yfhM → / ← PP_0571 lipoprotein/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947668,0920AT 8.7% 121.9 / 3.3 46intergenic (+56/+96)yfhM/PP_0571lipoprotein/membrane protein
Reads supporting (aligned to +/- strand):  ref base A (24/18);  new base T (2/2);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01

TGCCGCCGCCGCAGGTCGAATCGATGTACCGGCCGAACCTGCAGGCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCA  >  NC_002947/667948‑668232
                                                                                                                                                  |                                                                                                                                            
tGCCGCCGCCGCAGGTCGAATCGATGTACCGGCCGAACCTGCAGGCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCTGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCg                                                                                                                                          >  5:289453/1‑149 (MQ=255)
  ccgccgccgCAGGTCGAATCGATGTACCGGCCGAACCTGCAGGCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCgg                                                                                                                                         <  8:2805/148‑1 (MQ=255)
         cgcAGGTCGAATCGATGTACCGGCCGAACCTGCAGGCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCTTTGCGGccctatcg                                                                                                                                 <  1:3051/149‑6 (MQ=255)
              gTCGAATCGATGTACCGGCCGAACCTGCAGGCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGGCAAtta                                                                                                                             >  5:282819/1‑147 (MQ=255)
               tCGAATCGATGTACCGGCCGAACCTGCAGGCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCaa                                                                                                                           <  1:292643/149‑1 (MQ=255)
                cGAATCGATGTACCGGCCGAACCTGCAGGCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAg                                                                                                                          >  3:138825/1‑149 (MQ=255)
                   aTCGATGTACCGGCCGAACCTGCAGGCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAgcgg                                                                                                                       <  3:213687/149‑1 (MQ=255)
                          tACCGGCCGAACCTGCAGGCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCt                                                                                                                >  4:35313/1‑149 (MQ=255)
                            ccGGCCGAACCTGCAGGCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAg                                                                                                                          <  4:265700/137‑1 (MQ=255)
                            ccGGCCGAACCTGCAGGCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAg                                                                                                                          >  3:265700/1‑137 (MQ=255)
                             cGGCCGAACCTGCAGGCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGGCGTGGGTGGCGCGA‑‑GGGCCCCCAAGCGGCCCCCCCAAATAAAACGGCGACGcggg                                                                                                              >  1:319173/1‑145 (MQ=255)
                                ccGAACCTGCAGGCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGc                                                                                                          <  3:35313/149‑1 (MQ=255)
                                  gAACCTGCAGGCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCTTTGCGGccctatcg                                                                                                                                 <  8:17987/124‑6 (MQ=38)
                                  gAACCTGCAGGCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCTTTGCGGccctatcg                                                                                                                                 >  7:17987/1‑119 (MQ=38)
                                    aCCTGCAGGCCGTGGGCGATGGGCAAGGGGAGATGACCGTGAACGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTgg                                                                                                      <  1:244743/149‑1 (MQ=255)
                                      cTGCAGGCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTgggg                                                                                                    <  5:302932/149‑1 (MQ=255)
                                          aGGCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTg                                                                                                       >  8:111654/1‑142 (MQ=255)
                                          aGGCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTg                                                                                                       <  7:111654/142‑1 (MQ=255)
                                            gCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGgctgc                                                                                                >  5:257166/1‑147 (MQ=255)
                                            gCCGTGGGCGAGGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGTGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGgctgc                                                                                                <  6:257166/147‑1 (MQ=255)
                                                  ggCTATGGGCATGGGGATATCACGGTTAAAGCCCGATAGAGACCCGCTCTACGCGTAATGTGTGGGAGCGGCCTTGCGTCGCTATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAg                                                                                         <  4:138825/148‑1 (MQ=37)
                                                    cGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGGCTTGCGTGGGGGTA‑‑GGGCCGCAAAGCGGGCCCGCCCATTGAAGCGGCGAAGCTGAACGCCTGGGGCTGCTGTGCAGccc                                                                                      >  3:59585/1‑149 (MQ=255)
                                                    cGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAGccc                                                                                      >  5:288065/1‑149 (MQ=255)
                                                       tGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCT‑GGGCTGCTGTGCAGCCCTGTc                                                                                  <  3:125876/149‑1 (MQ=255)
                                                         ggCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTAGGGCTGCACAGCAGccccagg                                                                                  <  6:289453/148‑5 (MQ=37)
                                                          gCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTTCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTAAAGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTccc                                                                                >  5:76322/1‑147 (MQ=255)
                                                                          gTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATAGTGGGCCGCAAAGCGGCCCCGCCAATTAAAGCGGCGAAGCGGAAAGCCGGGGGGTGCGGTGCAGCCCTGTCGCGACGCAAGGCCGcg                                                                  >  1:257487/1‑148 (MQ=37)
                                                                          gTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTcc                                                                >  8:225068/1‑149 (MQ=255)
                                                                               aGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGAc                                                                             <  4:301259/131‑1 (MQ=255)
                                                                               aGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGAc                                                                             >  3:301259/1‑131 (MQ=255)
                                                                                      tAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCATTAGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTAAAGCGGCGAAGCTGAAATCCAGGGGCTGCTGTGGAGCCCAGTAGCCACGCAACGCCCCACCACCAAAGAcccc                                                    >  6:106794/1‑147 (MQ=25)
                                                                                          gaCCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGTAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACcgcg                                                   >  3:223441/1‑146 (MQ=255)
                                                                                           aCCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTcccc                                                              >  5:180892/1‑133 (MQ=255)
                                                                                               gCTCTCCGCGGAATGGGTGGGCGCGGCCTGGCGTGGCTATC‑‑GGGCCCAAAAGCGGCCCCTACAATTCAAGCGGCGAAGCTTAAAGCCGGGGGCGGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAg                                           <  2:257487/149‑1 (MQ=25)
                                                                                                        ggAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCCAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCt                                                                                     >  5:27496/1‑98 (MQ=255)
                                                                                                                  ggAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCtcatca                        <  7:225068/149‑1 (MQ=255)
                                                                                                                    aGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCAcc                      >  8:266556/1‑149 (MQ=255)
                                                                                                                      cGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCca                    >  1:181983/1‑149 (MQ=255)
                                                                                                                        tccTGGCGTCGCGATA‑‑GGGCCGAAAAGCGGCCCCGACAATTCACGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCcaca                  <  5:106794/148‑1 (MQ=255)
                                                                                                                         ccTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCca                                                              >  8:193979/1‑104 (MQ=255)
                                                                                                                         ccTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTgg                                        >  2:195286/1‑126 (MQ=255)
                                                                                                                         ccTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTgg                                        <  1:195286/126‑1 (MQ=255)
                                                                                                                               gTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAg           >  5:306537/1‑149 (MQ=255)
                                                                                                                                   cgATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCAc        >  2:4308/1‑148 (MQ=255)
                                                                                                                                       tcggggCCGCTTTGCGGCCCCGACAATTCAAGCGGCGAAGCTGCAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGCCGCAAGGCCGCTCCCAAAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCCCCCACACCCCCAGCAccag     >  7:299786/4‑149 (MQ=255)
                                                                                                                                          gggCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCAccagcca  <  2:181983/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
TGCCGCCGCCGCAGGTCGAATCGATGTACCGGCCGAACCTGCAGGCCGTGGGCGATGGGCAAGGGGAGATGACGGTGAAAGCCCGCTAGAGACCCGCTCTACGCGGAAAGTGTGGGAGCGGCCTTGCGTCGCGATA‑‑GGGCCGCAAAGCGGCCCCGACAATTCAAGCGGCGAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCA  >  NC_002947/667948‑668232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑