Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 670,786 A→G 6.1% Q743R (CAG→CGG) ‡ pbpC → peptidoglycan glycosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947670,7860AG 6.1% 189.9 / 2.6 65Q743R (CAG→CGG) ‡pbpCpeptidoglycan glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (29/32);  new base G (2/2);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

AGGCGCCGGCCAGTGCACCACCGCTGTCGATCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGACGAAAGCGGTCAGACGGCGCGGGTGGCGTTCCAGGTCAGTGAGTAGGTGTTCGACGACAGCCG  >  NC_002947/670639‑670930
                                                                                                                                                   |                                                                                                                                                 
ctgcgcCGGCCAGTGCACCACCGCTGTCGATCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAg                                                                                                                                                  >  4:77768/3‑149 (MQ=255)
     ccGGCCAGTGCACCACCGCTGTCGATCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGtt                                                                                                                                             <  2:158672/149‑1 (MQ=255)
      cGGCCAGTGCACCACCGCTGTCGATCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCAGCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTg                                                                                                                                            <  5:35734/149‑1 (MQ=255)
       ggCCAGTGCACCACCGCTGTCGATCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCTGGCCGTTga                                                                                                                                           <  3:967/149‑2 (MQ=255)
         ccAGTGCACCACCGCTGTCGATCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGt                                                                                                                                         <  3:314436/149‑1 (MQ=255)
           aGTGCACCACCGCTGTCGATCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCTGGCCGTTga                                                                                                                                           <  5:1048/145‑2 (MQ=255)
           aGTGCACCACCGCTGTCGATCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCTGGCCGTTga                                                                                                                                           >  6:1048/1‑144 (MQ=255)
             tGCACCACCGCTGTCGATCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGaaa                                                                                                                                     <  3:77768/149‑1 (MQ=255)
             tGCACCACCGCTGTCGATCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGaaa                                                                                                                                     <  4:100281/149‑1 (MQ=255)
             tGCACCACCGCTGTCGATCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGaaa                                                                                                                                     <  6:305697/149‑1 (MQ=255)
             tGCACCACCGCTGTCGATCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGaaa                                                                                                                                     >  5:305697/1‑149 (MQ=255)
                accaccGCTGTCGATCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAAcc                                                                                                                                   >  1:252906/1‑148 (MQ=255)
                 ccaccGCTGTCGATCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCa                                                                                                                                 <  2:101855/149‑1 (MQ=255)
                  caccGCTGTCGATCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAg                                                                                                                                >  7:55990/1‑149 (MQ=255)
                      gCTGTCGATCGTCGGCTTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGc                                                                                                                            <  2:16226/149‑1 (MQ=255)
                          tCGATCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGa                                                                                                                        >  6:263827/1‑149 (MQ=255)
                          tCGATCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGa                                                                                                                        <  5:201598/149‑1 (MQ=255)
                             aTCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAg                                                                                                                                <  7:162186/138‑1 (MQ=255)
                             aTCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAg                                                                                                                                >  8:162186/1‑138 (MQ=255)
                                 tcgGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCttg                                                                                                                 <  4:162617/149‑1 (MQ=255)
                                  cgGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCttgt                                                                                                                <  5:164684/149‑1 (MQ=255)
                                      gTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTgg                                                                                                                                           <  7:107478/118‑1 (MQ=255)
                                      gTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTgg                                                                                                                                           >  8:107478/1‑118 (MQ=255)
                                          gcCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGt                                                                                                        >  7:90766/1‑149 (MQ=255)
                                          gcCCAGGCGACAATCTGCGCCGCCCGACCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGt                                                                                                        >  1:167975/1‑149 (MQ=255)
                                            ccAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGttt                                                                                                      >  7:273481/1‑149 (MQ=255)
                                              aGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGc                                                                                                    >  1:13650/1‑149 (MQ=255)
                                                gCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCca                                                                                                  <  8:90766/149‑1 (MQ=255)
                                                gCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCca                                                                                                  <  1:302176/149‑1 (MQ=255)
                                                  gACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGt                                                                                                        >  8:266142/1‑141 (MQ=255)
                                                  gACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGt                                                                                                        <  7:266142/141‑1 (MQ=255)
                                                  gACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGc                                                                                                    >  2:32488/1‑145 (MQ=255)
                                                  gACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGc                                                                                                    <  1:32488/145‑1 (MQ=255)
                                                  gACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCcaca                                                                                                <  3:155459/149‑1 (MQ=255)
                                                        cTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTtgct                                                                                                            <  8:116727/131‑1 (MQ=255)
                                                        cTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTtgct                                                                                                            >  7:116727/1‑131 (MQ=255)
                                                           cgccgcCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCgg                                                                                          <  4:233070/146‑1 (MQ=255)
                                                            gccgccCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAg                                                                                      >  1:190970/1‑149 (MQ=255)
                                                              cgccCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCTGGCAGGc                                                                                    <  5:263827/149‑1 (MQ=255)
                                                              cgccCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGc                                                                                    >  2:96477/1‑149 (MQ=255)
                                                                 ccGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGTCAGGACAGCttgtt                                                                                                               <  7:223681/119‑1 (MQ=255)
                                                                 ccGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCttgtt                                                                                                               >  8:223681/1‑119 (MQ=255)
                                                                         caGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAgcg                                                                        >  7:278280/1‑149 (MQ=255)
                                                                          aGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAgcgc                                                                       >  1:194988/1‑149 (MQ=255)
                                                                            cACTGAGCCGTTGCAATTGCAGGTATCTTCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTAGTGAAGGCCGAA‑CTCAgcgcga                                                                     >  7:267658/1‑148 (MQ=255)
                                                                               tGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAACCCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTg                                                                   >  8:225344/1‑148 (MQ=255)
                                                                                     gTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGACGaaa                                                            <  2:13650/149‑1 (MQ=255)
                                                                                            ttGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGACGAAAGCGGTCa                                                     <  8:273481/149‑1 (MQ=255)
                                                                                                aGGTATCTGCCTTGGGTGGTGGTGGACGGCTCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGACGAAAGCGGTCAGACg                                                 >  4:147845/1‑149 (MQ=255)
                                                                                                         ccTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGACGAAAGCGGTCAGACGGCGCGGGTg                                        <  8:278280/149‑1 (MQ=255)
                                                                                                         ccTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGACGAAAGCGGTCAGACGGCGCGGGTg                                        >  2:40744/1‑149 (MQ=255)
                                                                                                             gggTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGAc                                                                >  7:257006/1‑121 (MQ=255)
                                                                                                             gggTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGAc                                                                <  8:257006/121‑1 (MQ=255)
                                                                                                             gggTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGACGAAAGCGGTCAGACGGcgcg                                            >  2:39117/1‑141 (MQ=255)
                                                                                                             gggTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGACGAAAGCGGTCAGACGGcgcg                                            >  8:64073/1‑141 (MQ=255)
                                                                                                             gggTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGACGAAAGCGGTCAGACGGcgcg                                            <  1:39117/141‑1 (MQ=255)
                                                                                                             gggTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGACGAAAGCGGTCAGACGGcgcg                                            <  7:64073/141‑1 (MQ=255)
                                                                                                                        gACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGACGAAAGCGGTCAGACGGCGCGGGTGGCGTTCCAGGTCAGt                         <  2:167975/149‑1 (MQ=255)
                                                                                                                        gACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGACGAAAGCGGTCAGACGGCGCGGGTGGCGTTCCAGGTCAGt                         <  2:194988/149‑1 (MQ=255)
                                                                                                                                 ggtggtTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGACGAAAGCGGTCAGACGGCGCGGGTGGCGTTCCAGGTCAGTGAGTAGgtg                >  8:279441/1‑149 (MQ=255)
                                                                                                                                  gtggtTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAAGCTCAGCGCGCTGGACGAAAGCGGTCAGACGGCGCGGGTGGCGTTCCAGGTCAGTGAGTAGgtg                <  1:96477/149‑1 (MQ=255)
                                                                                                                                          tCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGACGAAAGAGGTCAGACGGCGCGGGTGGCGTTCCAGGTCAGTGAGTAGCTGTTcgacga        >  3:258774/1‑148 (MQ=255)
                                                                                                                                          ccAACGGCTGGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGACGAAAGCGGTCAGACGGCGCGGGTGGCGTTCCAGGTCAGTGAGTAGGTGTTcgacga        >  1:307973/2‑148 (MQ=255)
                                                                                                                                              cGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGACGAAAGCGGTCAGACGGCGCGGGTGGCGTTCCAGGTCAGTGAGTAGGTGTTCGACGACAGcc   >  1:201760/1‑149 (MQ=255)
                                                                                                                                               ggCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGACGAAAGCGGTCAGACGGCGCGGGTGGCGTTCCAGGTCAGTGAGTAGGTGTTCGACGACAGCCg  <  1:40744/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AGGCGCCGGCCAGTGCACCACCGCTGTCGATCGTCGGCGTGCGCCCAGGCGACAATCTGCGCCGCCCGGCCACCAGCACTGAGCCGTTGCAATTGCAGGTATCTGCCTTGGGTGGTGGTGGACGGCGCTGGTGGTTCCTCAACGGCCAGCCGTTGGGTGAAACCCAGGGGCAGGACAGCTTGTTGCTGCGTTTGCCACAGGTCGGGCAGGCCGAA‑CTCAGCGCGCTGGACGAAAGCGGTCAGACGGCGCGGGTGGCGTTCCAGGTCAGTGAGTAGGTGTTCGACGACAGCCG  >  NC_002947/670639‑670930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑