Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 710,118 T→G 7.6% L288R (CTG→CGG)  ribF → bifunctional riboflavin kinase/FMN adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947710,1180TG 7.6% 155.7 / 2.8 51L288R (CTG→CGG) ribFbifunctional riboflavin kinase/FMN adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (26/21);  new base G (2/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATCGGCGTGCGCCCCACCGTTGCCGGTGATGGGCGCCCGCACCTGGAGATTCATCTTCTGGACTTTGCCGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGAAATGACCGACTACAAAGCCACGCTTAACCTTCCGGACACCGCCTTCCCCATGA  >  NC_002947/709974‑710254
                                                                                                                                                |                                                                                                                                        
atatCGGCGTGCGCCCCACCGTTGCCGGTGATGGGCGCCCGCACCTGGAGATTCATCTTCTGGACTTTGCCGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAg                                                                                                                                      <  7:77534/149‑1 (MQ=255)
      gCGTGCGCCCCACCGTTGCCGGTGATGGGCGCCCGCACCTGGAGATTCATCTTCTGGACTTTGCCGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCAGGGAGgcgaat                                                                                                                                <  3:153836/149‑4 (MQ=255)
        gTGCGCCCCACCGTTGCCGGTGATGGGCGCCCGCACCTGGAGATTCATCTTCTGGACTTTGCCGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGaa                                                                                                                              <  1:145065/149‑1 (MQ=255)
                         cGGTGATGGGCGCCCGCACCTGGAGATTCATCTTCTGGACTTTGCCGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCgg                                                                                                             <  6:136934/149‑1 (MQ=255)
                          ggTGATGGGCACCCGCACCTGGAGATTCATCTTCTGGACTTTGCCGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGc                                                                                                               >  4:163632/1‑146 (MQ=255)
                          ggTGATGGGCACCCGCACCTGGAGATTCATCTTCTGGACTTTGCCGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGc                                                                                                               <  3:163632/146‑1 (MQ=255)
                                                      aTCTTCTGGACTTTGCCGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCAc                                                                                <  8:186254/149‑1 (MQ=255)
                                                       tcttctGGACTTTGCCGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCAGGGAGgcgaatc                                                                                                                               >  5:201864/1‑97 (MQ=255)
                                                       tcttctGGACTTTGCCGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCAGGGAGgcgaatc                                                                                                                               <  6:201864/101‑5 (MQ=255)
                                                         ttctGGACTTTGCCGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCt                                                                             >  8:160955/1‑149 (MQ=255)
                                                         ttctGGACTTTGCCGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTCGAGGCGCTAAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGc                                                                                          >  4:27212/1‑136 (MQ=255)
                                                         ttctGGACTTTGCCGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTCGAGGCGCTAAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGc                                                                                          <  3:27212/136‑1 (MQ=255)
                                                             ggACTTTGCCGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAAc                                                                         >  8:120802/1‑149 (MQ=255)
                                                                 tttGCCGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGt                                                                                            >  6:157612/1‑126 (MQ=255)
                                                                 tttGCCGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGt                                                                                            <  5:157612/126‑1 (MQ=255)
                                                                 tttGCCGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGc                                                                      >  3:174762/1‑148 (MQ=255)
                                                                    gCCGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCAGAAGTCGGCGATCGATGCGTATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAAc                                                                  >  5:122698/1‑149 (MQ=255)
                                                                      cGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACg                                                                 >  3:5209/1‑148 (MQ=255)
                                                                       ggCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGaa                                                               <  4:5209/149‑1 (MQ=255)
                                                                         cGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATcgccgcc                                                                                                  <  3:294940/112‑1 (MQ=255)
                                                                         cGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATcgccgcc                                                                                                  >  4:294940/1‑112 (MQ=255)
                                                                              tttACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCt                                                         >  2:229257/1‑148 (MQ=255)
                                                                                tACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGc                                                                                          >  5:248807/1‑113 (MQ=255)
                                                                                tACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGc                                                                                          <  6:248807/113‑1 (MQ=255)
                                                                                tACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGa                                                       >  1:316683/1‑148 (MQ=255)
                                                                                      cggcGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGAAATGAcc                                                <  7:289978/149‑1 (MQ=255)
                                                                                         cgcCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGAAATGACCGAc                                             <  4:174762/149‑1 (MQ=255)
                                                                                           gcttgtggTGGAACTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGAGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGATCCTGAAATGACAGACt                                            >  8:116012/7‑148 (MQ=255)
                                                                                           ccTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGAAATGACCGACTa                                           >  3:262351/1‑149 (MQ=255)
                                                                                              gACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCg                                                                       <  4:59551/118‑1 (MQ=255)
                                                                                              gACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCg                                                                       >  3:59551/1‑118 (MQ=255)
                                                                                                 ggTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGaa                                                      >  5:78882/1‑132 (MQ=255)
                                                                                                 ggTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGaa                                                      <  6:78882/132‑1 (MQ=255)
                                                                                                      aGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGAAATGACCGACTACAAAGCCACGc                                >  2:306133/1‑149 (MQ=255)
                                                                                                          ccaccaCAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGAAATGACCGACTACAAAGCCACGCTTaa                            >  3:220380/1‑149 (MQ=255)
                                                                                                                   gCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGAAATGACCGACTACAAAGCCACGCTTAACCTTCCGGa                   <  2:316683/149‑1 (MQ=255)
                                                                                                                    cTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGc                                                           <  8:180259/108‑1 (MQ=255)
                                                                                                                    cTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGc                                                           >  7:180259/1‑108 (MQ=255)
                                                                                                                         tGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGAAATGACCGACTACAAAGCCACGCTTAACCTTCCGGACACCGc             >  1:159708/1‑149 (MQ=255)
                                                                                                                          gAAGAGCAGCGATTCGCCTCCCGGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGAAATGACCGACTACAAAGCCACGCTTAACCTTCCGGACACCGcc            >  7:203039/1‑149 (MQ=255)
                                                                                                                             gagCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGcgatcgat                                                                                                                 <  2:7820/45‑1 (MQ=255)
                                                                                                                             gagCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGcgatcgat                                                                                                                 >  1:7820/1‑45 (MQ=255)
                                                                                                                             gagCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCTGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGAAATGACCGACTACAAAGCCACGCTTAACCTTCCGGACACCGCCTTc         <  6:122698/149‑1 (MQ=255)
                                                                                                                               gcagcGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGAAATGACCGACTa                                           <  7:36180/113‑1 (MQ=255)
                                                                                                                               gcagcGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGAAATGACCGACTa                                           >  8:36180/1‑113 (MQ=255)
                                                                                                                                 agcGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCTCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGAAATGACCGACTACAAAGCCACGCTTAACCTTCCGGACACCGCCTTCCCCa     >  6:38748/1‑149 (MQ=255)
                                                                                                                                 agcGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGAAATGACCGACTACAAAGCCACGCTTAACCTTCCGGACACCGCCTTCCCCa     >  8:25458/1‑149 (MQ=255)
                                                                                                                                  gcGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGAAATGACCGACTACAAAGCCACGCTTAACCTTCCGGACACCGCCTTCCCCAt    >  2:279677/1‑149 (MQ=255)
                                                                                                                                    gATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGAAATGACCGACTACAAAGCCACGCTTAACCTTCCGGACACCGCCTTCCCCATGa  <  4:140684/149‑1 (MQ=255)
                                                                                                                                          ccTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGaaa                                                     >  4:23183/1‑92 (MQ=255)
                                                                                                                                          ccTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGaaa                                                     <  3:23183/92‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                        
ATATCGGCGTGCGCCCCACCGTTGCCGGTGATGGGCGCCCGCACCTGGAGATTCATCTTCTGGACTTTGCCGGCGATCTTTACGGCCGGCGCCTGACGGTGGAGTTCCACCACAAGCTGCGTGAAGAGCAGCGATTCGCCTCCCTGGAGGCGCTGAAGTCGGCGATCGATGCGGATATCGCCGCCGCACGTGCACATTGGCACGCTCAACCGCTAACGAAGAGCCTGAAATGACCGACTACAAAGCCACGCTTAACCTTCCGGACACCGCCTTCCCCATGA  >  NC_002947/709974‑710254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑