Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 733,851 T→C 8.8% intergenic (+21/+170) PP_t11 → / ← ndh tRNA‑Lys/NADH dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947733,8510TC 8.8% 121.5 / 2.9 46intergenic (+21/+170)PP_t11/ndhtRNA‑Lys/NADH dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (25/16);  new base C (2/2);  total (28/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

TTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGCCACCATCGCATTCACAGGTAAACCC  >  NC_002947/733705‑733994
                                                                                                                                                  |                                                                                                                                               
tttGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATga                                                                                                                                               >  7:159499/1‑149 (MQ=255)
                   gAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCtttt                                                                                                                                    <  6:315766/141‑1 (MQ=255)
                   gAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCtttt                                                                                                                                    >  5:315766/1‑141 (MQ=255)
                    aGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGcc                                                                                                                           >  8:181021/1‑149 (MQ=255)
                              cccTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATg                                                                                                                 >  8:154797/1‑149 (MQ=255)
                               ccTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATgc                                                                                                                <  6:258804/149‑1 (MQ=255)
                                          ccATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGgcg                                                                                                     <  6:119289/149‑1 (MQ=255)
                                             tttttGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTAGAATCCTACACGACACACCATGAACAAAGGGCATCTCGCATGACATGCCCTTATTCATTTGCCTCGAAAAATGCCCCCATCGGCGaaa                                                                                                  >  8:246701/1‑149 (MQ=21)
                                                  gggTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCa                                                                                             >  7:219135/1‑149 (MQ=255)
                                                   ggTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGAGCCCATCGGCGCAATCCCa                                                                                             >  3:214466/1‑148 (MQ=255)
                                                      cGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTg                                                                                         >  2:101499/1‑149 (MQ=255)
                                                        ttAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCaa                                                                                                  >  5:294180/1‑138 (MQ=255)
                                                        ttAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCaa                                                                                                  <  6:294180/138‑1 (MQ=255)
                                                           gCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATc                                                                                     >  8:160767/1‑148 (MQ=255)
                                                               aGTTTGTAGAGCACTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTAttt                                                                                <  5:45110/149‑1 (MQ=255)
                                                               aGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTAttt                                                                                <  8:159499/149‑1 (MQ=255)
                                                                      agagCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCt                                                                                          <  1:223435/132‑1 (MQ=255)
                                                                      agagCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCt                                                                                          >  2:223435/1‑132 (MQ=255)
                                                                           aGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGccc                                                                    <  7:154797/149‑1 (MQ=255)
                                                                           aGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTACCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGccc                                                                    <  7:119033/149‑1 (MQ=255)
                                                                                 cTTTTAACCAATTGGTCTTTGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGt                                                              <  7:160767/149‑1 (MQ=255)
                                                                                 cTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGt                                                              <  3:199637/149‑1 (MQ=255)
                                                                                    ttAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAg                                                            <  1:101499/148‑1 (MQ=255)
                                                                                       aCCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGc                                                        >  3:196418/1‑149 (MQ=255)
                                                                                        ccAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATg                                                               >  4:258068/1‑141 (MQ=255)
                                                                                        ccAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATg                                                               <  3:258068/141‑1 (MQ=255)
                                                                                                tCGTAGGTTCGAATCCTACACGACCCACCATGAACAACGGGCATCTCCCAGGAGATGCCCTTATTCTTTTGCCTCGAAAAATCCGCCCATCTGCGCAATCACCCCTGCATATATTTGCCACACAGCCACTaag                                                               >  5:32887/1‑131 (MQ=37)
                                                                                                      gTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGaa                                         >  3:168155/1‑149 (MQ=255)
                                                                                                      gTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCATCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGaa                                         >  5:14498/1‑149 (MQ=255)
                                                                                                       ttCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAAt                                        >  5:137375/1‑149 (MQ=255)
                                                                                                           aaTCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCgg                                    >  2:169489/1‑149 (MQ=255)
                                                                                                                tACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTg                               >  1:76545/1‑149 (MQ=255)
                                                                                                                      aCCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGcca                         >  6:7510/1‑149 (MQ=255)
                                                                                                                          accaTGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGCCACCAt                     >  2:226082/1‑149 (MQ=255)
                                                                                                                                 aCAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGCCACCATCGCAtt               >  2:112983/1‑148 (MQ=255)
                                                                                                                                    aaGGGCATCTCATGCGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAAt                                        <  4:192740/120‑1 (MQ=39)
                                                                                                                                    aaGGGCATCTCATGCGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAAt                                        >  3:192740/1‑120 (MQ=39)
                                                                                                                                     aGGGCATCTCATGCGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCcac                                                                           >  2:57880/1‑84 (MQ=38)
                                                                                                                                     aGGGCATCTCATGCGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCcac                                                                           <  1:57880/84‑1 (MQ=38)
                                                                                                                                           tctcGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGCCACCATCGCATTCACAGGTAAAc    >  7:153486/1‑149 (MQ=255)
                                                                                                                                             tcGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGCCACCATCGCATTCACAGGTAAAccc  >  3:307095/1‑149 (MQ=255)
                                                                                                                                               gCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATg                                       >  7:212720/1‑110 (MQ=255)
                                                                                                                                               gCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATg                                       <  8:212720/110‑1 (MQ=255)
                                                                                                                                               gCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGCCACCATCGCATTCACAg          <  6:181215/139‑1 (MQ=255)
                                                                                                                                               gCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGCCACCATCGCATTCACAg          >  5:181215/1‑139 (MQ=255)
                                                                                                                                               gCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGCCACCATCGCATTCACAGGTAAAcc   <  1:169489/146‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGCCACCATCGCATTCACAGGTAAACCC  >  NC_002947/733705‑733994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑