Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 744,162 C→T 8.7% G58G (GGG→GGA)  PP_0636 ← cold shock DNA‑binding domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947744,1620CT 8.7% 128.6 / 2.4 46G58G (GGG→GGA) PP_0636cold shock DNA‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (20/22);  new base T (2/2);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAAAAGTGAAAATGGTGTCGAAACCACGTGCCTTCGAATCTCTTGTCCATATGAAATGATTCGGAGACTCGTAGGCGTAGCCTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCAAACCCCTTCTCAGGCATGAAGCTGCGCACGACGCCTGAGGTGA  >  NC_002947/744016‑744304
                                                                                                                                                  |                                                                                                                                              
ccAAAAGTGAAAATGGTGTCGAAACCACGTGCCTTCGAATCTCTTGTCCATATGAAATGATTCGGAGACTCGTAGGCGTAGCCTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGc                                                                                                                                                <  3:257921/147‑1 (MQ=255)
 cAAAAGTGAAAATGGTGTCGAAACCACGTGCCTTCGAATCTCTTGTCCATATGAAATGATTCGGAGACTCGTAGGCGTAGCCTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCt                                                                                                                                             >  8:78780/1‑149 (MQ=255)
    aaGTGAAAATGGTGTCGAAACCACGTGCCTTCGAATCTCTTGTCCATATGAAATGATTCGGAGACTCGTAGGCGTAGCCTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCtt                                                                                                                                            <  4:283727/147‑1 (MQ=255)
    aaGTGAAAATGGTGTCGAAACCACGTGCCTTCGAATCTCTTGTCCATATGAAATGATTCGGAGACTCGTAGGCGTAGCCTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCtt                                                                                                                                            >  3:283727/1‑147 (MQ=255)
      gTGAAAATGGTGTCGAAACCACGTGCCTTCGAATCTCTTGTCCATATGAAATGATTCGGAGACTCGTAGGCGTAGCCTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCaaa                                                                                                                                        <  6:176076/149‑1 (MQ=255)
         aaaaTGGTGTCGAAACCACGTGCCTTCGAATCTCTTGTCCATATGAAATGATTCGGAGACTCGTAGGCGTAGCCTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGc                                                                                                                                     <  1:135420/149‑1 (MQ=255)
             tGGTGTCGAAACCACGTGCCTTCGAATCTCTTGTCCATATGAAATGATTCGGAGACTCGTAGGCGTAGCCTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTact                                                                                                                                 >  4:51368/1‑149 (MQ=255)
                    gAAACCACGTGCCTTCGAATCTCTTGTCCATATGAAATGATTCGGAGACTCGTAGGCGTAGCCTGGAGGCGGTGGGCTCTCCCCTGGTTTTCCCTTCACTGCTCTTTATCCTTTCTGACCCGGCTGTCATTCAAATGCTActgcctgca                                                                                                                          >  4:247132/1‑148 (MQ=255)
                    gAAACCACGTGCCTTCGAATCTCTTGTCCATATGAAATGATTCGGAGACTCGTAGGCGTAGCCTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGcc                                                                                                                          >  7:73568/1‑149 (MQ=255)
                    gAAACCACGTGCCTTCGAATCTCTTGTCCATATGAAATGATTCGGAGACTCGTAGGCGTAGCCTGGAGGCGGTGGGCGCTCACCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGTCCTTCAAATGCTACTGACTGc                                                                                                                           >  4:251414/1‑148 (MQ=255)
                        ccACGTGCCTTCGAATCTCTTGTCCATATGAAATGATTCGGAGACTCGTAGGCGTAGCCTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATc                                                                                                                      <  3:51368/149‑1 (MQ=255)
                                         tctTGTCCATATGAAATGATTCGGAGACTCGTAGGCGTAGCCTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGctct                                                                                                            >  3:244791/1‑142 (MQ=255)
                                         tctTGTCCATATGAAATGATTCGGAGACTCGTAGGCGTAGCCTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGctct                                                                                                            <  4:244791/142‑1 (MQ=255)
                                          ctTGTCCATATGAAATGATTCGGAGACTCGTAGGCGTAGCCTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGctc                                                                                                             >  7:163898/1‑140 (MQ=255)
                                          ctTGTCCATATGAAATGATTCGGAGACTCGTAGGCGTAGCCTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGctc                                                                                                             <  8:163898/140‑1 (MQ=255)
                                                           aTTCGGAGACTCGTAGGCGTAGCCTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGa                                                                                   >  5:156248/1‑149 (MQ=255)
                                                                         aGGCGTAGCCTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGt                                                                     >  7:142939/1‑149 (MQ=255)
                                                                             gTAGCCTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACaaaa                                                                              <  7:253907/136‑1 (MQ=255)
                                                                             gTAGCCTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACaaaa                                                                              >  8:253907/1‑136 (MQ=255)
                                                                                 ccTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTc                                                                                                                                           >  1:186342/1‑71 (MQ=255)
                                                                                 ccTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTc                                                                                                                                           <  2:186342/71‑1 (MQ=255)
                                                                                      aGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCtt                                                        <  6:58066/149‑1 (MQ=255)
                                                                                        gCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTgg                                                      <  7:78780/149‑1 (MQ=255)
                                                                                         cGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGa                                                     <  4:76463/149‑1 (MQ=255)
                                                                                              ggCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAg                                                <  2:20107/149‑1 (MQ=255)
                                                                                              ggCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAg                                                <  8:142939/149‑1 (MQ=255)
                                                                                                   tCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCaaa                                           >  8:286903/1‑149 (MQ=255)
                                                                                                     cccTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCAAAcc                                         >  8:285891/1‑149 (MQ=255)
                                                                                                       cTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCAAAcccc                                       <  7:285891/149‑1 (MQ=255)
                                                                                                            ttGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGAtgt                                                                                      >  2:50827/1‑97 (MQ=255)
                                                                                                            ttGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGAtgt                                                                                      <  1:50827/97‑1 (MQ=255)
                                                                                                               aCCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCTTATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTTGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCAAAccc                                        <  8:20341/140‑1 (MQ=255)
                                                                                                               aCCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCAAACCCCTTCTCAgg                               >  2:55568/1‑149 (MQ=255)
                                                                                                                 cTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCAAACCCCTTCTCAGGCa                             >  1:256581/1‑149 (MQ=255)
                                                                                                                 cTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCAAACCCCTTCTCAGGCa                             <  6:156248/149‑1 (MQ=255)
                                                                                                                 cTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCAAACCCCTTCTCAGGCa                             >  5:269829/1‑149 (MQ=255)
                                                                                                                        gCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCAAACCCCTTCTCAGGCATGAAGCt                      >  7:172450/1‑149 (MQ=255)
                                                                                                                         ctctGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCAAACCCCTTCTCAGGCa                             >  7:280899/1‑141 (MQ=255)
                                                                                                                         ctctGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCAAACCCCTTCTCAGGCa                             <  8:280899/141‑1 (MQ=255)
                                                                                                                            tGTATCCTTTCGGACCCGGCTGTCCTTCAAATGCTACTGACTGCCCATCGAACCGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCAAACCCCTTCTCAGGCATGAAGCTgcgc                  <  3:247132/149‑1 (MQ=255)
                                                                                                                            tGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCAAACCCCTTCTCa                                 <  5:54543/134‑1 (MQ=255)
                                                                                                                            tGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCAAACCCCTTCTCa                                 >  6:54543/1‑134 (MQ=255)
                                                                                                                            tGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCAAACCCCTTCTCAGGCATGAAGCTgcg                   >  4:264611/1‑148 (MQ=255)
                                                                                                                              tATCCTTTCGGACCCGGCTGTCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCAAACCCCTTCTCAGGCATGAAGCTgcgc                  <  3:251414/147‑1 (MQ=255)
                                                                                                                              tATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCGTTGGTTGAAGCCAAACGCGTTCTCAGGTATGAAGCTgggc                  <  8:73568/147‑1 (MQ=255)
                                                                                                                                     tCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCAAACCCCTTCTCAGGCATGAAGCTGCGCACGACGc           <  1:55568/147‑1 (MQ=255)
                                                                                                                                            cGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCAAACCCCTTCTCAGGCATGAAGCTGCGCACGACGCCtgaggtga  <  6:269829/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CCAAAAGTGAAAATGGTGTCGAAACCACGTGCCTTCGAATCTCTTGTCCATATGAAATGATTCGGAGACTCGTAGGCGTAGCCTGGAGGCGGTGGGCGCTCCCCTGGTTTGACCTTCACTGCTCTGTATCCTTTCGGACCCGGCTGCCCTTCAAATGCTACTGACTGCCCATCGAACAGCTCTCCTCCCTCTACGTTTTTGATGTGGACAAAATAGCTGCGTCCATCATCTCCTTGGATGAAGCCAAACCCCTTCTCAGGCATGAAGCTGCGCACGACGCCTGAGGTGA  >  NC_002947/744016‑744304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑