Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 787,196 A→G 5.5% D105G (GAC→GGC)  ettA → ADP/ATP ratio sensor/translation inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947787,1960AG 5.5% 219.8 / 2.2 73D105G (GAC→GGC) ettAADP/ATP ratio sensor/translation inhibitor
Reads supporting (aligned to +/- strand):  ref base A (39/30);  new base G (2/2);  total (41/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

CAAGGAATTCGACGGCGAAGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGCCGACGCCCTGCGCCTGC  >  NC_002947/787050‑787342
                                                                                                                                                  |                                                                                                                                                  
cAAGGAATTCGACGGCGAAGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGAc                                                                                                                                                   >  6:90469/1‑148 (MQ=255)
 aaGGAATTCGACGGCGAAGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGAc                                                                                                                                                   >  1:196382/1‑147 (MQ=255)
 aaGGAATTCGACGGCGAAGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGAc                                                                                                                                                   >  2:49049/1‑147 (MQ=255)
  aGGAATTCGACGGCGAAGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCGGGCCTGGGCgtc                                                                                                                                                <  2:6622/149‑3 (MQ=255)
  aGGAATTCGACGGCGAAGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAg                                                                                                                                                <  3:88195/149‑1 (MQ=255)
  aGGAATTCGACGGCGAAGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAg                                                                                                                                                <  6:257023/149‑1 (MQ=255)
          gACGGCGAAGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGGGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTAcgc                                                                                                                                        >  6:39488/1‑149 (MQ=255)
           aCGGCGAAGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTAcgcg                                                                                                                                       >  8:76313/1‑149 (MQ=255)
             ggCGAAGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCGGGCCTGGGCgt                                                                                                                                                 <  7:73181/137‑2 (MQ=255)
             ggCGAAGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCGGGCCTGGGCgt                                                                                                                                                 >  8:73181/1‑136 (MQ=255)
                 aaGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTAc                                                                                                                                 <  2:196382/149‑1 (MQ=255)
                     ccGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGcc                                                                                                                              >  8:168562/1‑148 (MQ=255)
                      cGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGcc                                                                                                                              >  2:203755/1‑147 (MQ=255)
                         ccGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGcc                                                                                                                         >  3:224024/1‑149 (MQ=255)
                                ccGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGcc                                                                                                                  <  1:49049/149‑1 (MQ=255)
                                 cGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGcc                                                                                                                  >  2:195885/1‑148 (MQ=255)
                                         aCGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGa                                                                                                          >  8:163028/1‑148 (MQ=255)
                                           gTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGAc                                                                                                         >  6:206701/1‑147 (MQ=255)
                                              ggTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCt                                                                                                    >  7:304880/1‑149 (MQ=255)
                                                  aCCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGgc                                                                                                 <  7:76313/148‑1 (MQ=255)
                                                  aCCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGgc                                                                                                 <  8:304880/148‑1 (MQ=255)
                                                    cTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCt                                                                                                    <  1:301443/143‑1 (MQ=255)
                                                    cTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCt                                                                                                    >  2:301443/1‑143 (MQ=255)
                                                       ccACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGa                                                                                           <  7:168562/149‑1 (MQ=255)
                                                          caGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAg                                                                                          >  1:307237/1‑147 (MQ=255)
                                                          caGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCa                                                                                        <  3:299338/149‑1 (MQ=255)
                                                           aGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAg                                                                                       >  3:207327/1‑149 (MQ=255)
                                                           aGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGGCGAGGAAGCGGTCAGCGTAATCATGGACGCCCAGGCCCGCCTTGACGAGGTCTACGAGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAg                                                                                       <  7:29170/149‑1 (MQ=255)
                                                             gAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGc                                                                                     >  3:277489/1‑149 (MQ=255)
                                                              aaCCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGcc                                                                                    <  3:58149/149‑1 (MQ=255)
                                                                         gACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAg                                                                          <  1:203755/148‑1 (MQ=255)
                                                                         gACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGGCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGaa                                                                           <  5:90469/147‑1 (MQ=255)
                                                                           cccAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCa                                                                       <  6:331632/149‑1 (MQ=255)
                                                                            ccaaccaaGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGGAGCCAt                                                                      >  6:284155/1‑149 (MQ=255)
                                                                             caaccaaGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTg                                                                                                   >  7:71460/1‑119 (MQ=255)
                                                                             caaccaaGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTg                                                                                                   <  8:71460/119‑1 (MQ=255)
                                                                             caaccaaGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCt                                                                               >  7:216951/1‑139 (MQ=255)
                                                                             caaccaaGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCt                                                                               <  8:216951/139‑1 (MQ=255)
                                                                                ccaaGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTg                                                                  <  2:307237/149‑1 (MQ=255)
                                                                                 caaGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTgg                                                                             <  4:263921/137‑1 (MQ=255)
                                                                                 caaGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTgg                                                                             >  3:263921/1‑137 (MQ=255)
                                                                                 caaGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGc                                                                 <  7:163028/149‑1 (MQ=255)
                                                                                  aaGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAg                                                                                 <  8:62259/132‑1 (MQ=255)
                                                                                  aaGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAg                                                                                 >  7:62259/1‑132 (MQ=255)
                                                                                        gtgcgtgAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGGAGCCATCCTGCAGgccgc                                                          <  5:284155/149‑1 (MQ=255)
                                                                                        gtgcgtgAAGGGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGgccgc                                                          <  5:39488/149‑1 (MQ=255)
                                                                                            gtgAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGAt                                                      >  8:201294/1‑149 (MQ=255)
                                                                                              gAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATgg                                                    >  4:144232/1‑149 (MQ=255)
                                                                                                aGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGc                                                   <  4:207327/148‑1 (MQ=255)
                                                                                                  tGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCca                                                 >  3:230131/1‑148 (MQ=255)
                                                                                                   ggTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCcac                                                >  6:99222/1‑148 (MQ=255)
                                                                                                    gTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGcc                                                                        <  2:270259/123‑1 (MQ=255)
                                                                                                    gTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGcc                                                                        >  1:270259/1‑123 (MQ=255)
                                                                                                        aGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACAAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTg                                          >  3:135091/1‑149 (MQ=255)
                                                                                                          gAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCGGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTg                                          >  1:263472/1‑147 (MQ=255)
                                                                                                          gAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCATGCCGCCGATGGCCACAACCTGGa                                        <  7:201294/149‑1 (MQ=255)
                                                                                                                    gCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTg                              >  2:199167/1‑149 (MQ=255)
                                                                                                                     cGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTg                              >  7:78815/1‑148 (MQ=255)
                                                                                                                      gTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGa                            >  8:133612/1‑149 (MQ=255)
                                                                                                                      gTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGa                            >  1:38028/1‑149 (MQ=255)
                                                                                                                            aaGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGc                      >  8:131439/1‑149 (MQ=255)
                                                                                                                             aGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCt                     <  1:195885/149‑1 (MQ=255)
                                                                                                                               ctcgtccAGGCGGGCCTGGGCGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGc                   >  3:77661/6‑149 (MQ=255)
                                                                                                                                    ccAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGCCGACg              >  6:65485/1‑149 (MQ=255)
                                                                                                                                          ccGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGCCGACGCCCTgc        >  7:231523/1‑149 (MQ=255)
                                                                                                                                          ccGCCTGGACAAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGCCGACGCCCTgc        <  4:135091/149‑1 (MQ=255)
                                                                                                                                            gCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGCCGACGCCCTgcgc      >  7:295021/1‑149 (MQ=255)
                                                                                                                                             ccTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGCCGACGCCCTGCGcc     >  3:90300/1‑149 (MQ=255)
                                                                                                                                             ccTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGCCGACGCCCTGCGcc     >  2:50083/1‑149 (MQ=255)
                                                                                                                                              cTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGCCGACGCCCTGCGCCt    <  7:194654/149‑1 (MQ=255)
                                                                                                                                              cTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGCCGACGCCCTGCGCCt    <  4:230131/149‑1 (MQ=255)
                                                                                                                                              cTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGCCGACGCCCTGCGCCt    >  1:136914/1‑149 (MQ=255)
                                                                                                                                                 gACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGCCGACGCCCTGCGCCTGc  <  4:90300/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CAAGGAATTCGACGGCGAAGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGCCGACGCCCTGCGCCTGC  >  NC_002947/787050‑787342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑