Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 802,770 T→C 7.9% intergenic (+28/‑58) obg → / → proB GTPase Obg/glutamate 5‑kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947802,7700TC 7.9% 180.2 / 4.7 63intergenic (+28/‑58)obg/proBGTPase Obg/glutamate 5‑kinase
Reads supporting (aligned to +/- strand):  ref base T (28/30);  new base C (3/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

CGCCCGTACCCTGCGTCGTACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGCCTCGACCGCGGT  >  NC_002947/802622‑802917
                                                                                                                                                    |                                                                                                                                                   
cgCCCGTACCCTGCGTCGTACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGctct                                                                                                                                                     <  5:184492/149‑1 (MQ=255)
 gCCCGTACCCTGCGTCGTACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGCTTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGctctc                                                                                                                                                    >  3:144152/1‑149 (MQ=255)
   ccGTACCCTGCGTCGTACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTg                                                                                                                                                  >  8:225195/1‑149 (MQ=255)
      tACCCTGCGTCGTACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAg                                                                                                                                               <  1:171973/149‑1 (MQ=255)
          cTGCGTCGTACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAgcggc                                                                                                                                           >  1:258896/1‑149 (MQ=255)
           tGCGTCGTACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAgcggcg                                                                                                                                          >  4:315440/1‑149 (MQ=255)
           tGCGTCGTACCGGCATCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGAGCGACTGATCGGGTGCAGTACACTAAACGCCGCTCTCTGGAgcgggg                                                                                                                                          >  8:297253/1‑147 (MQ=255)
                cgtACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCACGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGttttt                                                                                                                                     <  3:273150/149‑1 (MQ=255)
                 gtACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGttttt                                                                                                                                     <  4:200163/148‑1 (MQ=255)
                 gtACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGttttt                                                                                                                                     <  5:270845/148‑1 (MQ=255)
                 gtACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTg                                                                                                                                    <  7:225195/149‑1 (MQ=255)
                  tACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGATTTTg                                                                                                                                    >  5:160288/1‑148 (MQ=255)
                       gcatCAAGAGCGTGCATGCCATCGGCGCCGACGATGGTTGGGACGATGATTGCGAGGACCACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCa                                                                                                                              <  7:297253/146‑1 (MQ=255)
                       gCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCa                                                                                                                              <  3:315440/149‑1 (MQ=255)
                       gCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCa                                                                                                                              <  3:303204/149‑1 (MQ=255)
                          tCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACgg                                                                                                                           >  5:249730/1‑149 (MQ=255)
                           cAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGt                                                                                                                          <  6:310595/149‑1 (MQ=255)
                              gagCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATc                                                                                                                       >  8:30208/1‑149 (MQ=255)
                               agCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCt                                                                                                                      <  7:30208/149‑1 (MQ=255)
                                     cATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTAc                                                                                                                    <  2:241132/145‑1 (MQ=255)
                                     cATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTAc                                                                                                                    >  1:241132/1‑145 (MQ=255)
                                      aTGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCt                                                                                                                      <  5:175817/142‑1 (MQ=255)
                                      aTGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCt                                                                                                                      >  6:175817/1‑142 (MQ=255)
                                      aTGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGAtt                                                                                                               >  8:74004/1‑149 (MQ=255)
                                         aCATCGGGGACGACGATGGTTGGGACGAGGATTTCGTGGACGACGAAGACGGTGCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACTCTAAACGCCGCTGTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTct                                                                                                             <  4:114835/148‑2 (MQ=255)
                                         aCATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCg                                                                                                             <  5:255094/148‑1 (MQ=255)
                                           aTCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACa                                                                                                          >  1:212183/1‑149 (MQ=255)
                                                 gacgacgaTGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGtt                                                                                                    <  8:195752/149‑1 (MQ=255)
                                                 gacgacgaTGGTTGGGACGATGATTTAGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGAGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCAACAGTATCTACAGATTCGACaaaattt                                                                                                    >  4:246926/1‑143 (MQ=255)
                                                  acgacgaTGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTg                                                                                                   <  2:122263/149‑1 (MQ=255)
                                                   cgacgaTGGTTGTGACGATGATTTCGATGACGACGAAGACGGTCCGGAAATCATTTACGTGAGCGACTGATCGTTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTg                                                                                                   <  3:246926/148‑1 (MQ=255)
                                                           gTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGAt                                                                                          >  3:28371/1‑149 (MQ=255)
                                                                     tgatTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAg                                                                                <  2:212183/149‑1 (MQ=255)
                                                                      gatTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAgg                                                                               >  3:301534/1‑149 (MQ=255)
                                                                      gatTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAgg                                                                               >  2:185567/1‑149 (MQ=255)
                                                                       atTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGgaaga                                                                                              >  6:164191/1‑133 (MQ=255)
                                                                        tttCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGt                                                                              <  4:28371/148‑1 (MQ=255)
                                                                        tttCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTg                                                                             <  7:74004/149‑1 (MQ=255)
                                                                          tCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGgaaga                                                                                              >  3:220044/1‑130 (MQ=255)
                                                                          tCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGgaaga                                                                                              <  4:220044/130‑1 (MQ=255)
                                                                                  gacgaAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTgg                                                                                                  <  8:234482/118‑1 (MQ=255)
                                                                                   acgaAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTAAAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCaa                                                                  >  7:208726/1‑149 (MQ=255)
                                                                                      aaGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAgcg                                                               <  1:185567/149‑1 (MQ=255)
                                                                                           ggTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTggg                                                          <  4:301534/149‑1 (MQ=255)
                                                                                             tCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTc                                                        >  5:236349/1‑149 (MQ=255)
                                                                                                  aaaTCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTaa                                                   <  6:237394/149‑1 (MQ=255)
                                                                                                               gcgcGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGct                                      >  4:227637/1‑149 (MQ=255)
                                                                                                                  cgACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTctg                                   <  2:258896/149‑1 (MQ=255)
                                                                                                                   gACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTctgc                                  >  1:156926/1‑149 (MQ=255)
                                                                                                                            gTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAg                                          <  2:10606/132‑1 (MQ=255)
                                                                                                                            gTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAg                                          >  1:10606/1‑132 (MQ=255)
                                                                                                                               gCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATg                      >  6:285873/1‑149 (MQ=255)
                                                                                                                                cAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATg                      <  8:208726/148‑1 (MQ=255)
                                                                                                                                  gTACACTAAACGCCGCGCTCTGGAGCGGCGTTTTTGTATCCACGGGATCGACAGAGTCGACAGAGGTTGGAAGAAGATGCGAAGCAAGGTGCCGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCGCTGCTGACCGCCGATGGc                    >  5:160129/1‑148 (MQ=255)
                                                                                                                                      acTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAggg               >  4:209131/1‑149 (MQ=255)
                                                                                                                                      acTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCGGGGGCGGTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAggg               >  8:259046/1‑149 (MQ=255)
                                                                                                                                       aaaaaCGCCGCTCCAGAGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGAt                                                                                          <  6:154300/71‑1 (MQ=25)
                                                                                                                                       aaaaaCGCCGCTCCAGAGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGAt                                                                                          >  5:154300/3‑73 (MQ=25)
                                                                                                                                       aaaaaCGCCGCTCCAGAGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTa                                                    <  3:241692/109‑1 (MQ=39)
                                                                                                                                       aaaaaCGCCGCTCCAGAGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTa                                                    >  4:241692/3‑111 (MQ=39)
                                                                                                                                       aaaaaCGCCGCTCCAGAGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGc              >  1:65214/3‑149 (MQ=255)
                                                                                                                                          aaCGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGCCTc           <  6:236349/149‑1 (MQ=255)
                                                                                                                                             gccgcTCTCTGGAGCGGCGTTTTTGTATGCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGCCTCGAc        >  1:180427/1‑149 (MQ=255)
                                                                                                                                             gccgcTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGCCTCGAc        >  4:65340/1‑149 (MQ=255)
                                                                                                                                                gcTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGCCTCGACcgc     <  6:249730/149‑1 (MQ=255)
                                                                                                                                                    tctGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGCCTCGACCGCGGt  >  5:250929/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCCCGTACCCTGCGTCGTACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGCCTCGACCGCGGT  >  NC_002947/802622‑802917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑