Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	876,015	G→C	9.0%	noncoding (105/142 nt)	PP_mr65 ←	YybP‑YkoY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	876,015	0	G	C	9.0%	197.2 / 6.9	65	noncoding (105/142 nt)	PP_mr65	YybP‑YkoY
Reads supporting (aligned to +/- strand):  ref base G (28/31);  new base C (3/3);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGACCAACAGTGATTCCAGCATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCGGCAAGCGCTGTTTT  >  NC_002947/875869‑876159                                                                                                                                                   |                                                                                                                                                 gggACCAACAGTGATTCCAGCATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGc                                                                                                                                                >  6:307242/1‑149 (MQ=255)      cAACAGTGATTCCAGCATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCACGCCTGCa                                                                                                                                           >  8:119961/1‑149 (MQ=255)       aaCAGTGATTCCAGCATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGACCACGGTAAGCTGTACGTGTCATACGTCTAGTCAAACCCTCGATCCGTCTATCCGGATCTCTCAATGCACGCGAGCAt                                                                                                                                          >  7:90215/1‑149 (MQ=255)         cAGTGATTCCAGCATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCACGCCTGCAtgg                                                                                                                                        <  7:119961/149‑1 (MQ=255)          aGTGATTCCAGCATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCAtggt                                                                                                                                       >  6:191362/1‑149 (MQ=255)             gATTCCAGCATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCACCATGCACGCCTGCAtggaga                                                                                                                                     <  4:140271/148‑4 (MQ=255)               ttCCAGCATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTc                                                                                                                                  <  2:195801/149‑1 (MQ=255)               ttCCAGCATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCACGCCTGCAtg                                                                                                                                         >  1:172819/1‑142 (MQ=255)               ttCCAGCATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCACGCCTGCAtg                                                                                                                                         <  2:172819/142‑1 (MQ=255)                         aGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCAtggt                                                                                                                                       >  5:48724/1‑134 (MQ=255)                         aGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCAtggt                                                                                                                                       <  6:48724/134‑1 (MQ=255)                                cTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCtg                                                                                                                 >  4:53943/1‑149 (MQ=255)                                   cGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCtgt                                                                                                                <  6:326210/147‑1 (MQ=255)                                   cGGGGGCGGGTCAACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCtgt                                                                                                                <  6:101581/147‑1 (MQ=255)                                      gggCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGAc                                                                                                            >  3:126665/1‑148 (MQ=255)                                                acacGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTg                                                                                                 >  1:223881/1‑149 (MQ=255)                                                  acGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGAc                                                                                               >  5:34054/1‑149 (MQ=255)                                                  acGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGAc                                                                                               <  3:285661/149‑1 (MQ=255)                                                       tATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCaa                                                                                                         <  1:141592/134‑1 (MQ=255)                                                       tATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCaa                                                                                                         >  2:141592/1‑134 (MQ=255)                                                         tGACACGCACACCTTACCCGGGGCGGGGAGGCTGTACGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGTTCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTAcc                                                                                        >  1:101194/1‑149 (MQ=25)                                                             acGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGc                                                                                    <  3:53943/149‑1 (MQ=255)                                                                   aGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTg                                                                              >  8:74668/1‑149 (MQ=255)                                                                    gCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTgg                                                                             <  7:74668/149‑1 (MQ=255)                                                                    gCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTgg                                                                             <  5:191362/149‑1 (MQ=255)                                                                    gCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTgg                                                                             <  4:126665/149‑1 (MQ=255)                                                                     ccTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTggg                                                                            >  2:61497/1‑149 (MQ=255)                                                                     ccTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTggg                                                                            >  3:151900/1‑149 (MQ=255)                                                                      cTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGt                                                                           >  7:72394/1‑149 (MQ=255)                                                                      cTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGt                                                                           >  7:197726/1‑149 (MQ=255)                                                                       tCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCAtgg                                                                                                                                        >  5:12853/1‑87 (MQ=255)                                                                       tCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCAtgg                                                                                                                                        <  6:12853/87‑1 (MQ=255)                                                                       tCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTg                                                                          >  6:229768/1‑149 (MQ=255)                                                                         cccgccccgGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTg                                                                          <  6:34054/147‑1 (MQ=255)                                                                              cccgGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCgg                                                                   <  2:223881/149‑1 (MQ=255)                                                                                cgGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGga                                                                 <  8:197726/149‑1 (MQ=255)                                                                                    tAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGac                                                              >  3:295299/1‑148 (MQ=255)                                                                                        gtgtGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTc                                                         <  4:295299/149‑1 (MQ=255)                                                                                        gtgtGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTc                                                         <  5:229768/149‑1 (MQ=255)                                                                                        gtgtGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGACACTACTc                                                         >  8:285933/1‑149 (MQ=255)                                                                                             cGTGTCTTAGGTCTTGTCAAACCATCGATGAGTATATGGGGATCTCTCCATTCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTTGACCAAGTATGTTTACGCGTACCGGGCGAGCTGGGTGGTCGCGGGAGACTACTCCCCt                                                     <  8:90215/148‑1 (MQ=255)                                                                                              gtgtCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAg                                                   <  6:298111/149‑1 (MQ=255)                                                                                                 tCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTTCCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGAc                                                <  1:61497/149‑1 (MQ=255)                                                                                                        tCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCg                                          <  8:296162/148‑1 (MQ=255)                                                                                                        tCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCg                                          >  6:285473/1‑148 (MQ=255)                                                                                                            gTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGTCGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGa                                        <  1:89431/146‑1 (MQ=255)                                                                                                               aaaCCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCgg                                                                   >  6:136320/1‑116 (MQ=255)                                                                                                               aaaCCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCgg                                                                   <  5:136320/116‑1 (MQ=255)                                                                                                               aaaCCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTg                                   <  2:101194/148‑1 (MQ=255)                                                                                                               aaaCCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGc                                  <  4:151900/149‑1 (MQ=255)                                                                                                               aaaCCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGc                                  >  2:278565/1‑149 (MQ=255)                                                                                                                   ccTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAg                              <  8:72394/149‑1 (MQ=255)                                                                                                                    cTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAgg                             >  5:154980/1‑149 (MQ=255)                                                                                                                     tCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAgg                             >  1:177972/1‑148 (MQ=255)                                                                                                                                 tGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCg                <  2:177972/149‑1 (MQ=255)                                                                                                                                         tctcCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCGGCAAgcgc        >  2:214245/1‑149 (MQ=255)                                                                                                                                         tctcCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCGGCAAgcgc        >  4:32252/1‑149 (MQ=255)                                                                                                                                           tcCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCGGCAAGCGCTg      >  3:124012/1‑149 (MQ=255)                                                                                                                                            ccATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCGGCAAGCGCTGt     >  5:243895/1‑149 (MQ=255)                                                                                                                                             cATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCGGCAAGCGCTGtt    >  4:112581/1‑149 (MQ=255)                                                                                                                                             cATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCGGCAAGCGCTGtt    <  5:285473/149‑1 (MQ=255)                                                                                                                                             cATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCGGCAAGCGCTGt     <  1:278565/148‑1 (MQ=255)                                                                                                                                               tGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCGGCAAGCGCTGtttt  <  7:285933/149‑1 (MQ=255)                                                                                                                                               tGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCGGCAAGCGCTGtttt  <  4:88108/149‑1 (MQ=255)                                                                                                                                               tGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCGGCAAGCGCTGtttt  <  6:154980/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 GGGACCAACAGTGATTCCAGCATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCGGCAAGCGCTGTTTT  >  NC_002947/875869‑876159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑