Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 887,772 A→T 6.6% Q269L (CAG→CTG)  PP_0768 → TPR domain‑containing response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947887,7720AT 6.6% 227.9 / 5.1 76Q269L (CAG→CTG) PP_0768TPR domain‑containing response regulator
Reads supporting (aligned to +/- strand):  ref base A (31/39);  new base T (3/2);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

TTTACGAGCAGGCGCTCAAGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGTGCAGGCGTTGATGAGCAAGAAT  >  NC_002947/887625‑887920
                                                                                                                                                   |                                                                                                                                                    
tttACGAGCAGGCGCTCAAGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCa                                                                                                                                                      <  5:190003/148‑1 (MQ=255)
    cGAGCAGGCGCTCAAGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGa                                                                                                                                                 >  6:47927/1‑149 (MQ=255)
        cAGGCGCTCCAGGCGTTGCCGATGATGCCAGGGGTTTAGGATGGCATGGCCGAGGTGATGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTGGATGAAGCCGTGCGCCTGTCGCGTTTGTCGGTGCGTCGGCAGTCGACGCt                                                                                                                                             >  4:151994/1‑149 (MQ=255)
         aGGCGCTCAAGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTg                                                                                                                                            >  4:277139/1‑149 (MQ=255)
          ggCGCTCAAGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTgg                                                                                                                                           <  1:269986/149‑1 (MQ=255)
          ggCGCTCAAGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGACTGCCGACGcacc                                                                                                                                           <  5:273314/149‑4 (MQ=255)
            cgcTCAAGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGc                                                                                                                                         <  3:179569/149‑1 (MQ=255)
                 aaGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTc                                                                                                                                                   <  4:125485/134‑1 (MQ=255)
                 aaGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTc                                                                                                                                                   >  3:125485/1‑134 (MQ=255)
                  aGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCaa                                                                                                                                       <  3:267571/145‑1 (MQ=255)
                  aGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCaa                                                                                                                                       >  4:267571/1‑145 (MQ=255)
                  aGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTg                                                                                                                                   <  4:227816/149‑1 (MQ=255)
                  aGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTg                                                                                                                                   <  2:275121/149‑1 (MQ=255)
                        tCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGTGGGATACACGGCGCGCGCAGAACCTGCTTGAATAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGAAGCTGTGCAAGCTGGCCCTg                                                                                                                             >  7:289236/1‑149 (MQ=255)
                         cccGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTg                                                                                                                             <  7:112737/148‑1 (MQ=255)
                         cccGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTg                                                                                                                             >  5:20165/1‑148 (MQ=255)
                          ccGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGga                                                                                                                           <  6:20165/149‑1 (MQ=255)
                           cGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTg                                                                                                                             >  3:225025/1‑146 (MQ=255)
                             aTCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGaa                                                                                                                        <  2:66224/149‑1 (MQ=255)
                                 tGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACg                                                                                                                      >  3:314362/1‑147 (MQ=255)
                                 tGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGaa                                                                                                                    <  3:277139/149‑1 (MQ=255)
                                    cAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGAc                                                                                                                 <  7:109199/149‑1 (MQ=255)
                                        ccTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCg                                                                                                             <  2:273221/149‑1 (MQ=255)
                                         cTTTACTCTGTCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCTCGCGCAGAACCTGCTTGAAGAAGCGGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCga                                                                                                            <  8:289236/149‑1 (MQ=255)
                                               gATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGaa                                                                                                                    >  7:69098/1‑135 (MQ=255)
                                               gATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGaa                                                                                                                    <  8:69098/135‑1 (MQ=255)
                                               gATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAgcg                                                                                                       >  5:158895/1‑148 (MQ=255)
                                                atggcatggcCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCa                                                                                                     <  4:256405/149‑1 (MQ=255)
                                                   gcatggcCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATc                                                                                                   <  4:284634/148‑1 (MQ=255)
                                                     atggcCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCg                                                                                                  >  5:110304/1‑147 (MQ=255)
                                                      tggcCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAg                                                                                               <  6:110304/149‑1 (MQ=255)
                                                                  tggtggCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCAt                                                                                   >  6:25547/1‑149 (MQ=255)
                                                                       gCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAg                                                                                               >  7:46538/1‑132 (MQ=255)
                                                                       gCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAg                                                                                               <  8:46538/132‑1 (MQ=255)
                                                                        cTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTg                                                                             <  8:8383/149‑1 (MQ=255)
                                                                         tCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGaa                                                                                                                    <  5:108871/109‑1 (MQ=255)
                                                                         tCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGaa                                                                                                                    >  6:108871/1‑109 (MQ=255)
                                                                         tCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTg                                                                             >  5:329622/1‑148 (MQ=255)
                                                                           aGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAAc                                                                          <  6:124162/149‑1 (MQ=255)
                                                                           aGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAAc                                                                          <  5:47927/149‑1 (MQ=255)
                                                                            gggggATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGTCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAAcc                                                                         <  1:46718/149‑1 (MQ=255)
                                                                              gggATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAg                                                                       <  4:225025/149‑1 (MQ=255)
                                                                               ggATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCTAAGGCGTTCCGTCATGCAGTGAACCAgg                                                                      >  5:131055/1‑149 (MQ=255)
                                                                                gATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCCGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAggg                                                                     >  5:231113/1‑149 (MQ=255)
                                                                                    cccGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAgggg                                                                    >  4:239057/1‑146 (MQ=255)
                                                                                     ccGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCaga                                                                <  3:192547/149‑1 (MQ=255)
                                                                                      cGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCagag                                                               >  7:89320/1‑149 (MQ=255)
                                                                                        gcgcgcgcAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGtt                                                             <  6:158895/149‑1 (MQ=255)
                                                                                                       gCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATg                                              >  7:251784/1‑149 (MQ=255)
                                                                                                         ttGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGt                                                              >  4:37357/1‑131 (MQ=255)
                                                                                                         ttGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGt                                                              <  3:37357/131‑1 (MQ=255)
                                                                                                           gaagaaGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCag                                                                 <  8:170202/126‑1 (MQ=255)
                                                                                                           gaagaaGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCag                                                                 >  7:170202/1‑126 (MQ=255)
                                                                                                            aagaagCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCga                                                                                                            >  5:87427/1‑82 (MQ=255)
                                                                                                            aagaagCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCga                                                                                                            <  6:87427/82‑1 (MQ=255)
                                                                                                             agaagCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGaaa                                        <  5:25547/149‑1 (MQ=255)
                                                                                                              gaagCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGaaaa                                       >  3:116268/1‑149 (MQ=255)
                                                                                                                agCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAaaca                                     <  3:151994/149‑1 (MQ=255)
                                                                                                                  ccGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGtt                                                             <  6:123632/123‑1 (MQ=255)
                                                                                                                  ccGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGtt                                                             >  5:123632/1‑123 (MQ=255)
                                                                                                                  ccGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAaacaac                                   >  5:9690/1‑149 (MQ=255)
                                                                                                                   cGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGTGCAAGCTGGAGCTGGAGAACGAAGACTTCGATAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGATTTCGCGCTACAAGGATGCCGAAaacaac                                   <  1:297630/148‑1 (MQ=255)
                                                                                                                           ggTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGTCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTg                          <  6:231113/148‑1 (MQ=255)
                                                                                                                             tCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTg                          <  8:89320/147‑1 (MQ=255)
                                                                                                                               gCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAaacaa                                    >  6:327140/1‑135 (MQ=255)
                                                                                                                               gCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGTg                       >  4:107851/1‑148 (MQ=255)
                                                                                                                                     gTCGGTGCGTCGGCAGTCGACGCTGGGCGAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGt                        >  2:81644/1‑141 (MQ=255)
                                                                                                                                     gTCGGTGCGTCGGCAGTCGACGCTGGGCGAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGt                        <  1:81644/141‑1 (MQ=255)
                                                                                                                                     gTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTa                                                      >  7:47629/1‑111 (MQ=255)
                                                                                                                                     gTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTa                                                      <  8:47629/111‑1 (MQ=255)
                                                                                                                                       cccagCGTCGACTGCCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCCTGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGTGCAGGCGtt               >  5:235576/5‑148 (MQ=255)
                                                                                                                                        ccagCGTCGACTGCCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGaaaa                                       >  6:4230/4‑123 (MQ=255)
                                                                                                                                        ccagCGTCGACTGCCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGaaaa                                       <  5:4230/120‑1 (MQ=255)
                                                                                                                                         gTGGGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGTGCAGGCGTtgat            <  6:9690/149‑1 (MQ=255)
                                                                                                                                         gTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCAGCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGTGCAGGCGTtgat            <  6:329622/149‑1 (MQ=255)
                                                                                                                                         cagCGTCGACTGCCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAAACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTAGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGTGCAGGCGTtgat            >  4:172945/3‑149 (MQ=255)
                                                                                                                                             gTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGTGCAGGCGTTGATGAg         >  3:135184/1‑148 (MQ=255)
                                                                                                                                                   aGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGTGCAGGCGTTGATGAGCAAGAAt  <  3:239057/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTTACGAGCAGGCGCTCAAGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGTGCAGGCGTTGATGAGCAAGAAT  >  NC_002947/887625‑887920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑