Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 887,774 T→C 6.4% S270P (TCG→CCG)  PP_0768 → TPR domain‑containing response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947887,7740TC 6.4% 227.8 / 3.6 77S270P (TCG→CCG) PP_0768TPR domain‑containing response regulator
Reads supporting (aligned to +/- strand):  ref base T (32/40);  new base C (3/2);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAGCAGGCGCTCAAGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGTGCAGGCGTTGATGAGCAAGAAT  >  NC_002947/887629‑887920
                                                                                                                                                 |                                                                                                                                                  
cGAGCAGGCGCTCAAGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGa                                                                                                                                                 >  6:47927/1‑149 (MQ=255)
    cAGGCGCTCCAGGCGTTGCCGATGATGCCAGGGGTTTAGGATGGCATGGCCGAGGTGATGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTGGATGAAGCCGTGCGCCTGTCGCGTTTGTCGGTGCGTCGGCAGTCGACGCt                                                                                                                                             >  4:151994/1‑149 (MQ=255)
     aGGCGCTCAAGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTg                                                                                                                                            >  4:277139/1‑149 (MQ=255)
      ggCGCTCAAGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTgg                                                                                                                                           <  1:269986/149‑1 (MQ=255)
      ggCGCTCAAGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGACTGCCGACGcacc                                                                                                                                           <  5:273314/149‑4 (MQ=255)
        cgcTCAAGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGc                                                                                                                                         <  3:179569/149‑1 (MQ=255)
             aaGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTc                                                                                                                                                   <  4:125485/134‑1 (MQ=255)
             aaGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTc                                                                                                                                                   >  3:125485/1‑134 (MQ=255)
              aGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCaa                                                                                                                                       >  4:267571/1‑145 (MQ=255)
              aGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCaa                                                                                                                                       <  3:267571/145‑1 (MQ=255)
              aGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTg                                                                                                                                   <  2:275121/149‑1 (MQ=255)
              aGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTg                                                                                                                                   <  4:227816/149‑1 (MQ=255)
                    tCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGTGGGATACACGGCGCGCGCAGAACCTGCTTGAATAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGAAGCTGTGCAAGCTGGCCCTg                                                                                                                             >  7:289236/1‑149 (MQ=255)
                     cccGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTg                                                                                                                             >  5:20165/1‑148 (MQ=255)
                     cccGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTg                                                                                                                             <  7:112737/148‑1 (MQ=255)
                      ccGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGga                                                                                                                           <  6:20165/149‑1 (MQ=255)
                       cGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTg                                                                                                                             >  3:225025/1‑146 (MQ=255)
                         aTCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGaa                                                                                                                        <  2:66224/149‑1 (MQ=255)
                             tGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACg                                                                                                                      >  3:314362/1‑147 (MQ=255)
                             tGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGaa                                                                                                                    <  3:277139/149‑1 (MQ=255)
                                cAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGAc                                                                                                                 <  7:109199/149‑1 (MQ=255)
                                    ccTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCg                                                                                                             <  2:273221/149‑1 (MQ=255)
                                     cTTTACTCTGTCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCTCGCGCAGAACCTGCTTGAAGAAGCGGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCga                                                                                                            <  8:289236/149‑1 (MQ=255)
                                           gATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGaa                                                                                                                    >  7:69098/1‑135 (MQ=255)
                                           gATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGaa                                                                                                                    <  8:69098/135‑1 (MQ=255)
                                           gATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAgcg                                                                                                       >  5:158895/1‑148 (MQ=255)
                                            atggcatggcCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCa                                                                                                     <  4:256405/149‑1 (MQ=255)
                                               gcatggcCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATc                                                                                                   <  4:284634/148‑1 (MQ=255)
                                                 atggcCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCg                                                                                                  >  5:110304/1‑147 (MQ=255)
                                                  tggcCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAg                                                                                               <  6:110304/149‑1 (MQ=255)
                                                              tggtggCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCAt                                                                                   >  6:25547/1‑149 (MQ=255)
                                                                   gCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAg                                                                                               >  7:46538/1‑132 (MQ=255)
                                                                   gCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAg                                                                                               <  8:46538/132‑1 (MQ=255)
                                                                    cTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTg                                                                             <  8:8383/149‑1 (MQ=255)
                                                                     tCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGaa                                                                                                                    >  6:108871/1‑109 (MQ=255)
                                                                     tCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGaa                                                                                                                    <  5:108871/109‑1 (MQ=255)
                                                                     tCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTg                                                                             >  5:329622/1‑148 (MQ=255)
                                                                       aGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAAc                                                                          <  6:124162/149‑1 (MQ=255)
                                                                       aGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAAc                                                                          <  5:47927/149‑1 (MQ=255)
                                                                        gggggATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGTCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAAcc                                                                         <  1:46718/149‑1 (MQ=255)
                                                                          gggATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAg                                                                       <  4:225025/149‑1 (MQ=255)
                                                                           ggATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCTAAGGCGTTCCGTCATGCAGTGAACCAgg                                                                      >  5:131055/1‑149 (MQ=255)
                                                                            gATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCCGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAggg                                                                     >  5:231113/1‑149 (MQ=255)
                                                                                cccGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAgggg                                                                    >  4:239057/1‑146 (MQ=255)
                                                                                 ccGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCaga                                                                <  3:192547/149‑1 (MQ=255)
                                                                                  cGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCagag                                                               >  7:89320/1‑149 (MQ=255)
                                                                                    gcgcgcgcAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGtt                                                             <  6:158895/149‑1 (MQ=255)
                                                                                                   gCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATg                                              >  7:251784/1‑149 (MQ=255)
                                                                                                     ttGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGt                                                              >  4:37357/1‑131 (MQ=255)
                                                                                                     ttGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGt                                                              <  3:37357/131‑1 (MQ=255)
                                                                                                       gaagaaGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCag                                                                 >  7:170202/1‑126 (MQ=255)
                                                                                                       gaagaaGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCag                                                                 <  8:170202/126‑1 (MQ=255)
                                                                                                        aagaagCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCga                                                                                                            >  5:87427/1‑82 (MQ=255)
                                                                                                        aagaagCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCga                                                                                                            <  6:87427/82‑1 (MQ=255)
                                                                                                         agaagCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGaaa                                        <  5:25547/149‑1 (MQ=255)
                                                                                                          gaagCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGaaaa                                       >  3:116268/1‑149 (MQ=255)
                                                                                                            agCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAaaca                                     <  3:151994/149‑1 (MQ=255)
                                                                                                              ccGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGtt                                                             <  6:123632/123‑1 (MQ=255)
                                                                                                              ccGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGtt                                                             >  5:123632/1‑123 (MQ=255)
                                                                                                              ccGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAaacaac                                   >  5:9690/1‑149 (MQ=255)
                                                                                                               cGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGTGCAAGCTGGAGCTGGAGAACGAAGACTTCGATAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGATTTCGCGCTACAAGGATGCCGAAaacaac                                   <  1:297630/148‑1 (MQ=255)
                                                                                                                       ggTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGTCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTg                          <  6:231113/148‑1 (MQ=255)
                                                                                                                         tCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTg                          <  8:89320/147‑1 (MQ=255)
                                                                                                                           gCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAaacaa                                    >  6:327140/1‑135 (MQ=255)
                                                                                                                           gCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGTg                       >  4:107851/1‑148 (MQ=255)
                                                                                                                                 gTCGGTGCGTCGGCAGTCGACGCTGGGCGAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGt                        >  2:81644/1‑141 (MQ=255)
                                                                                                                                 gTCGGTGCGTCGGCAGTCGACGCTGGGCGAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGt                        <  1:81644/141‑1 (MQ=255)
                                                                                                                                 gTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTa                                                      >  7:47629/1‑111 (MQ=255)
                                                                                                                                 gTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTa                                                      <  8:47629/111‑1 (MQ=255)
                                                                                                                                   cccagCGTCGACTGCCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCCTGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGTGCAGGCGtt               >  5:235576/5‑148 (MQ=255)
                                                                                                                                    ccagCGTCGACTGCCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGaaaa                                       >  6:4230/4‑123 (MQ=255)
                                                                                                                                    ccagCGTCGACTGCCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGaaaa                                       <  5:4230/120‑1 (MQ=255)
                                                                                                                                     gTGGGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGTGCAGGCGTtgat            <  6:9690/149‑1 (MQ=255)
                                                                                                                                     gTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCAGCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGTGCAGGCGTtgat            <  6:329622/149‑1 (MQ=255)
                                                                                                                                     cagCGTCGACTGCCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAAACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTAGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGTGCAGGCGTtgat            >  4:172945/3‑149 (MQ=255)
                                                                                                                                         gTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGTGCAGGCGTTGATGAg         >  3:135184/1‑148 (MQ=255)
                                                                                                                                               aGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGTGCAGGCGTTGATGAGCAAGAAt  <  3:239057/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CGAGCAGGCGCTCAAGGCGTTCCCGATCATGCCAGGCCTTTACGATGGCATGGCCGAGGTGCTGGTGGCTCAGGGGGATACCCGGCGCGCGCAGAACCTGCTTGAAGAAGCCGTGCGCCTGTCGCCTTTGTCGGTGCGTCGGCAGTCGACGCTGGGCAAGCTGGCGCTGGAGAACGAAGACTTCGAGAGCGCATCGAAGGCGTTCCGTCATGCAGTGAACCAGGGGCAGAGTTCGCGCTACAAGGATGCCGAAAACAACCTTGGCCTGGTGCAGGCGTTGATGAGCAAGAAT  >  NC_002947/887629‑887920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑