Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 901,418 G→A 8.2% intergenic (‑137/‑194) cysZ ← / → trxB sulfate transport protein CysZ/thioredoxin reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947901,4180GA 8.2% 133.2 / 2.5 49intergenic (‑137/‑194)cysZ/trxBsulfate transport protein CysZ/thioredoxin reductase
Reads supporting (aligned to +/- strand):  ref base G (22/22);  new base A (2/2);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.88e-01

TAGAGACTCCGCAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTGGGTCCTTTTTAATTTCTTGGCTGGCGC  >  NC_002947/901283‑901562
                                                                                                                                       |                                                                                                                                                
tAGAGACTCCGCAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGt                                                                                                                                     >  1:196709/1‑149 (MQ=255)
tAGAGAATCCGCAGAGAAGGAAAACTCGCTGACCTTACCGAGTTTGCCGCGCAGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCATCATAGGCTATGCCTATGAGGt                                                                                                                                     >  6:277841/1‑149 (MQ=255)
  gagaCTCCGCAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGt                                                                                                                                     <  1:40938/147‑1 (MQ=255)
  gagaCTCCGCAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTg                                                                                                                                    <  8:73740/148‑1 (MQ=255)
   agaCTCCGCAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACG‑AAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGAt                                                                                                                                 >  8:138348/1‑149 (MQ=255)
       tCCGCAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTg                                                                                                                               >  3:252730/1‑148 (MQ=255)
            agagAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAg                                                                                                                         <  3:133641/149‑1 (MQ=255)
             gagaAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAgg                                                                                                                        <  8:81976/149‑1 (MQ=255)
             gagaAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAgg                                                                                                                        <  2:196709/149‑1 (MQ=255)
             gagaAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAg                                                                                                                         >  7:53959/1‑148 (MQ=255)
              agaAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCATCGCCTATg                                                                                                                                         >  2:252534/1‑131 (MQ=255)
              agaAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCATCGCCTATg                                                                                                                                         <  1:252534/131‑1 (MQ=255)
                   gAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATAttt                                                                                                                  >  1:154441/1‑149 (MQ=255)
                    aaaaCGCGCTGACCTTACCGAGTTTGCCGCGCCGACG‑AAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTcc                                                                                                                <  7:138348/149‑1 (MQ=255)
                        cgcgCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTa                                                                                                             <  4:124116/149‑1 (MQ=255)
                         gcgcTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTaa                                                                                                            >  8:29951/1‑149 (MQ=255)
                               aacTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGAGCGTGAAACGTGATGTAACAAAAGCTCCCGGTGCTTGTTTATCGATGAAATCGCATCATAGGCGATGCCTATGATGTGGATTGTCTAAGGATATTTCCTTAATCttt                                                                                                       <  5:277841/146‑1 (MQ=255)
                               aCCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCttt                                                                                                       <  4:252730/148‑1 (MQ=255)
                               aCCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTg                                                                                                      >  8:99711/1‑149 (MQ=255)
                                  ttACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGa                                                                                                   >  4:178623/1‑149 (MQ=255)
                                              ccGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGt                                                                                       >  8:277931/1‑149 (MQ=255)
                                              ccGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGt                                                                                       <  7:202521/149‑1 (MQ=255)
                                               cgcgCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTg                                                                                      >  6:11785/1‑149 (MQ=255)
                                                  gcCGACTAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCTGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTgcgc                                                                                   <  6:87514/149‑1 (MQ=255)
                                                           aGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACtttt                                                                          <  7:99711/149‑1 (MQ=255)
                                                                     tGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCgg                                                                <  7:277931/149‑1 (MQ=255)
                                                                        gCGGGTAACGTGATGTAACAAAAGCGCGCGGGGCTGGTTTATCGATGATATGGCAGCATAGGCAATGACTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGc                                                              <  4:223732/148‑1 (MQ=255)
                                                                         cGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTCGTGTATCGATGAAATCGCAGCATAGGCAATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGATTTGCGGGcc                                                             >  3:223732/1‑148 (MQ=255)
                                                                                 gggATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGttt                                                    <  3:178623/149‑1 (MQ=255)
                                                                                 gggATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGttt                                                    <  2:154441/149‑1 (MQ=255)
                                                                                        aaCAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAAt                                                                                                           >  1:116356/1‑87 (MQ=255)
                                                                                        aaCAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAAt                                                                                                           <  2:116356/87‑1 (MQ=255)
                                                                                         aCAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTc                                            >  5:90128/1‑149 (MQ=255)
                                                                                          cAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTcc                                           >  3:195799/1‑149 (MQ=255)
                                                                                            aaaGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTccc                                          >  6:232803/1‑148 (MQ=255)
                                                                                              tgctcccGTGTCTTGTTTATCGATGATATCTCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCaa                                       <  8:271524/145‑1 (MQ=255)
                                                                                               gcgcCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAg                                      >  4:222051/1‑149 (MQ=255)
                                                                                                cgcCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGt                                     >  1:122098/1‑149 (MQ=255)
                                                                                                cgcCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGt                                     <  7:29951/149‑1 (MQ=255)
                                                                                                     ggggCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGtgctgc                                >  6:200475/1‑149 (MQ=255)
                                                                                                         cTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTgg                            <  8:53959/149‑1 (MQ=255)
                                                                                                                aTCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTc                                            >  2:290888/1‑126 (MQ=255)
                                                                                                                aTCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTc                                            <  1:290888/126‑1 (MQ=255)
                                                                                                                aTCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTGGGTCCttt                     >  7:121871/1‑149 (MQ=255)
                                                                                                                aTCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTGGGTCCttt                     >  5:297100/1‑149 (MQ=255)
                                                                                                                 tCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTc                                            >  1:269954/1‑125 (MQ=255)
                                                                                                                 tCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTc                                            <  2:269954/125‑1 (MQ=255)
                                                                                                                  cGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTGGGTCCtttt                    >  8:116576/1‑148 (MQ=255)
                                                                                                                                    ggCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTGGGTCCTTTTTAATTTCTTGGCTGgcgc  <  6:297100/148‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                                
TAGAGACTCCGCAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTGGGTCCTTTTTAATTTCTTGGCTGGCGC  >  NC_002947/901283‑901562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑