Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	904,643	T→G	6.0%	V301G (GTG→GGG)	PP_0788 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	904,643	0	T	G	6.0%	216.0 / 4.1	67	V301G (GTG→GGG)	PP_0788	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (34/29);  new base G (2/2);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

CGCAGGGCAGGCGTTGTTTGCCTCGTTGCAGGCACTGCTGGCGCCGGTGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGAAGACATGATCAACCTGGTTGGCCTGCTGTTCGCGTT  >  NC_002947/904497‑904790                                                                                                                                                   |                                                                                                                                                     cgcAGGGCAGGCGTTGTTTGCCTCGTTGCAGGCACTGCTGGCGCCGGTGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGCCCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGc                                                                                                                                                    >  1:217746/1‑149 (MQ=255) cgcAGGGCAGGCGTTGTTTGCCTCGTTGCAGGCACTGCTGGCGCCGGTGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGc                                                                                                                                                    >  5:22870/1‑149 (MQ=255) cgcAGGGCAGGCGTTGTTTGCCTCGTTGCAGGCACTGCTGGCGCCGGTGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGc                                                                                                                                                    >  6:301888/1‑149 (MQ=255)  gcagggcaggCGTTGTTTGCCTCGTTGCAGGCACTGCTGGCGCCGGTGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGcc                                                                                                                                                   >  1:77750/1‑149 (MQ=255)  gcagggcaggCGTTGTTTGCCTCGTTGCAGGCAATGCTGGCGCCGGTGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCAGCCCAGCCGATCAGTACCGCCGACCTGCTCAGGCTGCTGTCGCATTTGCAGCGCTATGTGcc                                                                                                                                                   >  1:81976/1‑149 (MQ=255)          ggCGTTGTTTGCCTCGTTGCAGGCACTGCTGGCGCCGGTGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACg                                                                                                                                            >  7:189456/1‑148 (MQ=255)           gCGTTGTTTGCCTCGTTGCAGGCACTGCTGGCGCCGGTGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTa                                                                                                                                          >  1:235137/1‑149 (MQ=255)           gCGTTGTTTGCCTCGTTGCAGGCACTGCTGGCGCCGGTGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTa                                                                                                                                          >  4:193731/1‑149 (MQ=255)                 tttGCCTCGTTGCAGGCACTGCTGGCGCCGGTGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAgg                                                                                                                                     <  7:195184/148‑1 (MQ=255)                  ttGCCTCGTTGCAGGCACTGCTGGCGCCGGTGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGcc                                                                                                                                   >  6:279571/1‑149 (MQ=255)                     ccTCGTTGCAGGCACTGCTGGCGCCGGTGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGa                                                                                                                                 <  4:202987/148‑1 (MQ=255)                      cTCGTTGCAGGCACTGCTGGCGCCGGTGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGAt                                                                                                                                >  4:278056/1‑148 (MQ=255)                                  aCTGCTGGCGCCGGTGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCg                                                                                                                  <  5:301888/149‑1 (MQ=255)                                  aCTGCTGGCGCCGGTGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGGGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCg                                                                                                                  <  8:208915/149‑1 (MQ=255)                                      ctgGCGCCGGTGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCca                                                                                                              <  3:193731/149‑1 (MQ=255)                                         gcgcCGGTGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCcagca                                                                                                           <  2:40797/149‑1 (MQ=255)                                            ccGGTGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCt                                                                                                        <  6:170004/149‑1 (MQ=255)                                                tGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGag                                                                                                    <  2:235137/149‑1 (MQ=255)                                                tGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGag                                                                                                    <  5:279571/149‑1 (MQ=255)                                                      ggCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAgctg                                                                                              <  6:136808/149‑1 (MQ=255)                                                           ttCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGGGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGtt                                                                                         >  2:153534/1‑149 (MQ=255)                                                           ttCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGGGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGtt                                                                                         >  6:262340/1‑149 (MQ=255)                                                               cTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCgg                                                                                     >  1:153872/1‑149 (MQ=255)                                                                  cgcgCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGt                                                                                   >  6:61627/1‑148 (MQ=255)                                                                   gcgcTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCgg                                                                                     >  2:262372/1‑145 (MQ=255)                                                                   gcgcTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCgg                                                                                     <  1:262372/145‑1 (MQ=255)                                                                           aaGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTa                                                                                                                                          <  5:241864/85‑1 (MQ=255)                                                                           aaGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTa                                                                                                                                          >  6:241864/1‑85 (MQ=255)                                                                           aaGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCgg                                                                                     >  3:163994/1‑137 (MQ=255)                                                                           aaGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCgg                                                                                     <  4:163994/137‑1 (MQ=255)                                                                           aaGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTgcg                                                                          >  4:159505/1‑148 (MQ=255)                                                                               ggTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGGGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCg                                                                     <  5:262340/149‑1 (MQ=255)                                                                               ggTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGGGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCg                                                                     <  1:153534/149‑1 (MQ=255)                                                                                   gCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTAc                                                                 >  2:279181/1‑149 (MQ=255)                                                                                     tGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCg                                                                     <  5:60947/143‑1 (MQ=255)                                                                                     tGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCg                                                                     >  6:60947/1‑143 (MQ=255)                                                                                     tGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTAcgc                                                               >  4:131734/1‑149 (MQ=255)                                                                                     tGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTAcgc                                                               >  3:220826/1‑149 (MQ=255)                                                                                     tGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTAcgc                                                               >  7:297563/1‑149 (MQ=255)                                                                                       ccgccCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTAcgc                                                               <  5:61627/147‑1 (MQ=255)                                                                                       ccgccCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGt                                                             >  8:84029/1‑149 (MQ=255)                                                                                           ccAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACTATCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGc                                                                                       <  1:214472/120‑1 (MQ=255)                                                                                           ccAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGc                                                                                       >  2:214472/1‑119 (MQ=255)                                                                                              gCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGcc                                                      <  6:8241/149‑1 (MQ=255)                                                                                                       tACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTgg                                             >  4:210685/1‑149 (MQ=255)                                                                                                          cgccgACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCt                                           >  4:106482/1‑148 (MQ=255)                                                                                                             cgACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCCAGCTCGTCCAGCAGCTTGATCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACg                                       <  6:267742/149‑1 (MQ=255)                                                                                                              gACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGctgctggccga                                                                                      >  8:110362/1‑96 (MQ=255)                                                                                                              gACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGctgctggccga                                                                                      <  7:110362/101‑6 (MQ=255)                                                                                                              gACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACg                                       >  4:204117/1‑148 (MQ=255)                                                                                                              gACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGa                                      <  7:84029/149‑1 (MQ=255)                                                                                                               aCCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGaa                                     <  6:22870/149‑1 (MQ=255)                                                                                                                 cTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGAAg                                    >  8:75012/1‑148 (MQ=255)                                                                                                                 cTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGAAGa                                   <  1:279181/149‑1 (MQ=255)                                                                                                                 cTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGAAGa                                   <  2:77750/149‑1 (MQ=255)                                                                                                                 cTGCTCCGGCTGATGTCGCATTTGCATCGCTATGTGCCCGCCACGTACGAGGCCGATTACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGAAGa                                   <  2:81976/149‑1 (MQ=255)                                                                                                                   gCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTAAGAGGCCGATGACT‑TCGAGCTCGGACAGCAGCTTGCGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGAAGACa                                 >  3:194066/1‑149 (MQ=255)                                                                                                                    cTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGAAGAc                                  <  8:189456/147‑1 (MQ=255)                                                                                                                            gctgTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGAAGACATGATCAAcc                        >  6:233727/1‑149 (MQ=255)                                                                                                                                tagCATTTTCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCTGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGAAGACATGATCAACCttg                     <  4:194066/146‑3 (MQ=255)                                                                                                                                tCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGAAGACATGATCAACCTgg                     <  3:278056/148‑1 (MQ=255)                                                                                                                                  gCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGAAGACATGATCAACCTGGTTg                  >  2:154258/1‑149 (MQ=255)                                                                                                                                         cAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGAAGACATGATCAACCTGGTTGGcc               >  6:227887/1‑145 (MQ=255)                                                                                                                                         cAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGAAGACATGATCAACCTGGTTGGcc               <  5:227887/145‑1 (MQ=255)                                                                                                                                         cAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGAAGACATGATCAACCTGGTTGGCctgct           >  6:302693/1‑149 (MQ=255)                                                                                                                                              cTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGAAGACATGATc                            >  7:254708/1‑127 (MQ=255)                                                                                                                                              cTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGAAGACATGATc                            <  8:254708/127‑1 (MQ=255)                                                                                                                                                   tgCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGAAGACATGATCAACCTGGTTGGCCTGCTGTTCGCGtt  <  3:204117/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     CGCAGGGCAGGCGTTGTTTGCCTCGTTGCAGGCACTGCTGGCGCCGGTGCGCGGGCAGTTCGCTCCGCGCTTGCAAGCGGTGGCTGCCGCCCAGCCGATCAGTACCGCCGACCTGCTCCGGCTGCTGTCGCATTTGCAGCGCTATGTGCCCGCCACGTACGAGGCCGATGACT‑TCGAGCTCGGCCAGCAGCTTGAGCAGCTGCTGTTGCGGGTCAGCGTGCGCAGCGGTACGCGTCGACGCCTTCACGTGGCTGACGAAGACATGATCAACCTGGTTGGCCTGCTGTTCGCGTT  >  NC_002947/904497‑904790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑