Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,013,235 A→T 9.9% intergenic (+63/+68) prfC → / ← potF‑I peptide chain release factor 3/putrescine‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,013,2350AT 9.9% 88.6 / 3.8 41intergenic (+63/+68)prfC/potF‑Ipeptide chain release factor 3/putrescine‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (19/16);  new base T (2/2);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.50e-02

GGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAG  >  NC_002947/1013091‑1013383
                                                                                                                                                |                                                                                                                                                    
ggCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGAcc                                                                                                                                                  >  6:284921/1‑149 (MQ=255)
    ccGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGa                                                                                                                                              <  5:284921/149‑1 (MQ=255)
                aaCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATa                                                                                                                                  <  8:77453/149‑1 (MQ=255)
                 aCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAc                                                                                                                                 <  3:205025/149‑1 (MQ=255)
                  ccTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCAACAGACCTGGAGGTCATGTGATTcc                                                                                                                                >  7:89588/1‑149 (MQ=255)
                    tGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAc                                                                                                                                 >  3:165757/1‑146 (MQ=255)
                    tGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAc                                                                                                                                 <  4:165757/146‑1 (MQ=255)
                      gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtg                                                                                                                              <  6:169368/147‑1 (MQ=25)
                      gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgtg                                                                                                                            <  2:107734/149‑1 (MQ=255)
                       cgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTgg                                                                                                                           <  8:88130/149‑1 (MQ=25)
                           gATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCGCCCACAGGAATAACCACACCAGGAGGTGATGTGTTTACGGTGGGAGc                                                                                                                       >  3:165804/1‑149 (MQ=38)
                                                     aGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGGCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAgg                                                                                             >  7:71516/1‑149 (MQ=255)
                                                       ttCCGCGCTACCCGCGAGCACCATTAAGGCCTAAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAggcg                                                                                           >  8:235233/1‑149 (MQ=255)
                                                          cgcgCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCCCCCCCACCCCCACGCATAACCCGGCCGGGGGGGGATTTGGTACCGGGGGGGGCGGGCGGGGCCCCGCAAGGCCCCGAggcgggc                                                                                        >  3:188250/1‑147 (MQ=255)
                                                                        gaaCCATTAAGGCCTTAAATCGGGGCTGCTTTGCAGCCCTTCGCTGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGGGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTcc                                                                          <  8:71516/147‑1 (MQ=255)
                                                                        gCACCATTAAGGCCTAAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTcc                                                                          <  7:235233/149‑1 (MQ=255)
                                                                                aaGGCCTTAAAGCGGGCCTGCTTTCCAGCCCTGGGCGGCCCCGCCCGCTCCCACCGTAACCCCCCGCCCTGTCGGTATTGTTAAACCTGTGGGTGCGGGGGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTg                                                                   <  4:188250/148‑1 (MQ=255)
                                                                                    ccTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCCCAACCACACGAATCACCAGACCTAGAGCTCAAGTGATACCTTTGGGAGCGGGCCTGCCCGCGAAGCGCCGCTAGGCGGCCCCAATTAAATTTAACGCTTTGATa                                                                >  4:259821/1‑147 (MQ=25)
                                                                                      tGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGaa                                                                                                        >  8:224360/1‑105 (MQ=255)
                                                                                      tGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGaa                                                                                                        <  7:224360/105‑1 (MQ=255)
                                                                                      tGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTgg                                                            >  4:244536/1‑149 (MQ=255)
                                                                                       gAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACCCCCCCGCCCACACGAATAACCAGGCCGGGAGGTGATGTGGTACCAGGGGGAGCGGGCGGGCCCGCGCAGGGCCCCAAGGCGGCCCCCAGTACATTTCCGGCGTTGATACTGGt                                                           >  6:174616/1‑149 (MQ=255)
                                                                                             ggggCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACTGTAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGa                                                      <  7:269922/148‑1 (MQ=255)
                                                                                               ggCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACGGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTc                                                   >  5:156639/1‑149 (MQ=255)
                                                                                                     tttGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTAc                                             <  7:287704/149‑1 (MQ=255)
                                                                                                     tttGCAGCCCTTCGCGGGCACCCCCGCACCCACAGGAATAACCAGACCTGGAGGTCATGGGATACCAGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCAATTCAATTTCCGGCGTTGTTAATGGTGCAGCTTCGCGTAc                                             >  8:287704/1‑149 (MQ=25)
                                                                                                            cccTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGGAGGGGCGCTAGGCGGCCCCATTTGAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTAc                                             >  1:264402/1‑142 (MQ=255)
                                                                                                             ccTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgcg                                                >  4:223596/1‑138 (MQ=255)
                                                                                                             ccTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgcg                                                <  3:223596/138‑1 (MQ=255)
                                                                                                                 cgcgGGCACGCCCGCTCCCACATGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTc                                   <  6:249041/147‑1 (MQ=255)
                                                                                                                    gggCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCt                              <  3:259821/149‑1 (MQ=255)
                                                                                                                          gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTcc                                                         <  2:116527/116‑1 (MQ=255)
                                                                                                                          gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTcc                                                         >  1:116527/1‑116 (MQ=255)
                                                                                                                          gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGa                                           >  6:306743/1‑130 (MQ=255)
                                                                                                                          gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGa                                           <  5:306743/130‑1 (MQ=255)
                                                                                                                          gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTACGGCTTTGATACTGGTCAAGATTCGCGTACGAATACGGTCGATCTTCGCCg                        >  8:250409/1‑149 (MQ=255)
                                                                                                                          gCCCGCTCCCAAAGTAATCACCAGTCCTGTATGTCATGTGTATCCTGTGGGAGCGGGCGTGCCCGGGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGTCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCg                        <  5:280476/149‑1 (MQ=38)
                                                                                                                                tCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCg                  >  8:118712/1‑149 (MQ=255)
                                                                                                                                  ccACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGcc                >  3:239903/1‑149 (MQ=25)
                                                                                                                                    acaGGTATCACATGACCTCCAGGTCTGGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTGGCCGGCATCGCCTc              >  3:182081/1‑149 (MQ=25)
                                                                                                                                              ccAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCAAGATTCGCGTACGAATACGGTCGATCTTGGCCGGCATAGCGTCCAGCGCATa     >  6:253942/1‑148 (MQ=255)
                                                                                                                                                aGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAg  >  5:154504/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAG  >  NC_002947/1013091‑1013383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑