Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,013,241 G→C 11.3% intergenic (+69/+62) prfC → / ← potF‑I peptide chain release factor 3/putrescine‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,013,2410GC11.3% 103.7 / 5.5 41intergenic (+69/+62)prfC/potF‑Ipeptide chain release factor 3/putrescine‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (19/17);  new base C (2/2);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.65e-01

CCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAG  >  NC_002947/1013095‑1013383
                                                                                                                                                  |                                                                                                                                              
ccGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGa                                                                                                                                              <  5:284921/149‑1 (MQ=255)
            aaCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATa                                                                                                                                  <  8:77453/149‑1 (MQ=255)
             aCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAc                                                                                                                                 <  3:205025/149‑1 (MQ=255)
              ccTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCAACAGACCTGGAGGTCATGTGATTcc                                                                                                                                >  7:89588/1‑149 (MQ=255)
                tGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAc                                                                                                                                 >  3:165757/1‑146 (MQ=255)
                tGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAc                                                                                                                                 <  4:165757/146‑1 (MQ=255)
                  gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtg                                                                                                                              <  6:169368/147‑1 (MQ=25)
                  gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgtg                                                                                                                            <  2:107734/149‑1 (MQ=255)
                   cgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTgg                                                                                                                           <  8:88130/149‑1 (MQ=25)
                       gATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCGCCCACAGGAATAACCACACCAGGAGGTGATGTGTTTACGGTGGGAGc                                                                                                                       >  3:165804/1‑149 (MQ=38)
                                                 aGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGGCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAgg                                                                                             >  7:71516/1‑149 (MQ=255)
                                                   ttCCGCGCTACCCGCGAGCACCATTAAGGCCTAAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAggcg                                                                                           >  8:235233/1‑149 (MQ=255)
                                                      cgcgCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCCCCCCCACCCCCACGCATAACCCGGCCGGGGGGGGATTTGGTACCGGGGGGGGCGGGCGGGGCCCCGCAAGGCCCCGAggcgggc                                                                                        >  3:188250/1‑147 (MQ=255)
                                                                    gaaCCATTAAGGCCTTAAATCGGGGCTGCTTTGCAGCCCTTCGCTGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGGGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTcc                                                                          <  8:71516/147‑1 (MQ=255)
                                                                    gCACCATTAAGGCCTAAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTcc                                                                          <  7:235233/149‑1 (MQ=255)
                                                                            aaGGCCTTAAAGCGGGCCTGCTTTCCAGCCCTGGGCGGCCCCGCCCGCTCCCACCGTAACCCCCCGCCCTGTCGGTATTGTTAAACCTGTGGGTGCGGGGGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTg                                                                   <  4:188250/148‑1 (MQ=255)
                                                                                ccTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCCCAACCACACGAATCACCAGACCTAGAGCTCAAGTGATACCTTTGGGAGCGGGCCTGCCCGCGAAGCGCCGCTAGGCGGCCCCAATTAAATTTAACGCTTTGATa                                                                >  4:259821/1‑147 (MQ=25)
                                                                                  tGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGaa                                                                                                        >  8:224360/1‑105 (MQ=255)
                                                                                  tGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGaa                                                                                                        <  7:224360/105‑1 (MQ=255)
                                                                                  tGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTgg                                                            >  4:244536/1‑149 (MQ=255)
                                                                                   gAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACCCCCCCGCCCACACGAATAACCAGGCCGGGAGGTGATGTGGTACCAGGGGGAGCGGGCGGGCCCGCGCAGGGCCCCAAGGCGGCCCCCAGTACATTTCCGGCGTTGATACTGGt                                                           >  6:174616/1‑149 (MQ=255)
                                                                                         ggggCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACTGTAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGa                                                      <  7:269922/148‑1 (MQ=255)
                                                                                           ggCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACGGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTc                                                   >  5:156639/1‑149 (MQ=255)
                                                                                                 tttGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTAc                                             <  7:287704/149‑1 (MQ=255)
                                                                                                 tttGCAGCCCTTCGCGGGCACCCCCGCACCCACAGGAATAACCAGACCTGGAGGTCATGGGATACCAGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCAATTCAATTTCCGGCGTTGTTAATGGTGCAGCTTCGCGTAc                                             >  8:287704/1‑149 (MQ=25)
                                                                                                        cccTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGGAGGGGCGCTAGGCGGCCCCATTTGAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTAc                                             >  1:264402/1‑142 (MQ=255)
                                                                                                         ccTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgcg                                                >  4:223596/1‑138 (MQ=255)
                                                                                                         ccTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgcg                                                <  3:223596/138‑1 (MQ=255)
                                                                                                             cgcgGGCACGCCCGCTCCCACATGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTc                                   <  6:249041/147‑1 (MQ=255)
                                                                                                                gggCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCt                              <  3:259821/149‑1 (MQ=255)
                                                                                                                      gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTcc                                                         >  1:116527/1‑116 (MQ=255)
                                                                                                                      gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTcc                                                         <  2:116527/116‑1 (MQ=255)
                                                                                                                      gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGa                                           >  6:306743/1‑130 (MQ=255)
                                                                                                                      gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGa                                           <  5:306743/130‑1 (MQ=255)
                                                                                                                      gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTACGGCTTTGATACTGGTCAAGATTCGCGTACGAATACGGTCGATCTTCGCCg                        >  8:250409/1‑149 (MQ=255)
                                                                                                                      gCCCGCTCCCAAAGTAATCACCAGTCCTGTATGTCATGTGTATCCTGTGGGAGCGGGCGTGCCCGGGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGTCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCg                        <  5:280476/149‑1 (MQ=38)
                                                                                                                            tCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCg                  >  8:118712/1‑149 (MQ=255)
                                                                                                                              ccACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGcc                >  3:239903/1‑149 (MQ=25)
                                                                                                                                acaGGTATCACATGACCTCCAGGTCTGGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTGGCCGGCATCGCCTc              >  3:182081/1‑149 (MQ=25)
                                                                                                                                          ccAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCAAGATTCGCGTACGAATACGGTCGATCTTGGCCGGCATAGCGTCCAGCGCATa     >  6:253942/1‑148 (MQ=255)
                                                                                                                                            aGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAg  >  5:154504/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAG  >  NC_002947/1013095‑1013383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑