Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,013,248 A→T 11.4% intergenic (+76/+55) prfC → / ← potF‑I peptide chain release factor 3/putrescine‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,013,2480AT11.4% 99.6 / 4.4 42intergenic (+76/+55)prfC/potF‑Ipeptide chain release factor 3/putrescine‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (20/17);  new base T (2/3);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.04e-01

AACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCAC  >  NC_002947/1013107‑1013395
                                                                                                                                             |                                                                                                                                                   
aaCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATa                                                                                                                                              <  8:77453/149‑1 (MQ=255)
 aCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAc                                                                                                                                             <  3:205025/149‑1 (MQ=255)
  ccTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCAACAGACCTGGAGGTCATGTGATTcc                                                                                                                                            >  7:89588/1‑149 (MQ=255)
    tGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAc                                                                                                                                             <  4:165757/146‑1 (MQ=255)
    tGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAc                                                                                                                                             >  3:165757/1‑146 (MQ=255)
      gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCtg                                                                                                                                          <  6:169368/147‑1 (MQ=25)
      gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgtg                                                                                                                                        <  2:107734/149‑1 (MQ=255)
       cgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTgg                                                                                                                                       <  8:88130/149‑1 (MQ=25)
           gATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCGCCCACAGGAATAACCACACCAGGAGGTGATGTGTTTACGGTGGGAGc                                                                                                                                   >  3:165804/1‑149 (MQ=38)
                                     aGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGGCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAgg                                                                                                         >  7:71516/1‑149 (MQ=255)
                                       ttCCGCGCTACCCGCGAGCACCATTAAGGCCTAAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAggcg                                                                                                       >  8:235233/1‑149 (MQ=255)
                                          cgcgCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCCCCCCCACCCCCACGCATAACCCGGCCGGGGGGGGATTTGGTACCGGGGGGGGCGGGCGGGGCCCCGCAAGGCCCCGAggcgggc                                                                                                    >  3:188250/1‑147 (MQ=255)
                                                        gaaCCATTAAGGCCTTAAATCGGGGCTGCTTTGCAGCCCTTCGCTGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGGGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTcc                                                                                      <  8:71516/147‑1 (MQ=255)
                                                        gCACCATTAAGGCCTAAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTcc                                                                                      <  7:235233/149‑1 (MQ=255)
                                                                aaGGCCTTAAAGCGGGCCTGCTTTCCAGCCCTGGGCGGCCCCGCCCGCTCCCACCGTAACCCCCCGCCCTGTCGGTATTGTTAAACCTGTGGGTGCGGGGGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTg                                                                               <  4:188250/148‑1 (MQ=255)
                                                                    ccTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCCCAACCACACGAATCACCAGACCTAGAGCTCAAGTGATACCTTTGGGAGCGGGCCTGCCCGCGAAGCGCCGCTAGGCGGCCCCAATTAAATTTAACGCTTTGATa                                                                            >  4:259821/1‑147 (MQ=25)
                                                                      tGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGaa                                                                                                                    >  8:224360/1‑105 (MQ=255)
                                                                      tGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGaa                                                                                                                    <  7:224360/105‑1 (MQ=255)
                                                                      tGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTgg                                                                        >  4:244536/1‑149 (MQ=255)
                                                                       gAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACCCCCCCGCCCACACGAATAACCAGGCCGGGAGGTGATGTGGTACCAGGGGGAGCGGGCGGGCCCGCGCAGGGCCCCAAGGCGGCCCCCAGTACATTTCCGGCGTTGATACTGGt                                                                       >  6:174616/1‑149 (MQ=255)
                                                                             ggggCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACTGTAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGa                                                                  <  7:269922/148‑1 (MQ=255)
                                                                               ggCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACGGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTc                                                               >  5:156639/1‑149 (MQ=255)
                                                                                     tttGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTAc                                                         <  7:287704/149‑1 (MQ=255)
                                                                                     tttGCAGCCCTTCGCGGGCACCCCCGCACCCACAGGAATAACCAGACCTGGAGGTCATGGGATACCAGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCAATTCAATTTCCGGCGTTGTTAATGGTGCAGCTTCGCGTAc                                                         >  8:287704/1‑149 (MQ=25)
                                                                                            cccTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGGAGGGGCGCTAGGCGGCCCCATTTGAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTAc                                                         >  1:264402/1‑142 (MQ=255)
                                                                                             ccTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgcg                                                            <  3:223596/138‑1 (MQ=255)
                                                                                             ccTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgcg                                                            >  4:223596/1‑138 (MQ=255)
                                                                                                 cgcgGGCACGCCCGCTCCCACATGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTc                                               <  6:249041/147‑1 (MQ=255)
                                                                                                    gggCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCt                                          <  3:259821/149‑1 (MQ=255)
                                                                                                          gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTcc                                                                     >  1:116527/1‑116 (MQ=255)
                                                                                                          gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTcc                                                                     <  2:116527/116‑1 (MQ=255)
                                                                                                          gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGa                                                       >  6:306743/1‑130 (MQ=255)
                                                                                                          gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGa                                                       <  5:306743/130‑1 (MQ=255)
                                                                                                          gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTACGGCTTTGATACTGGTCAAGATTCGCGTACGAATACGGTCGATCTTCGCCg                                    >  8:250409/1‑149 (MQ=255)
                                                                                                          gCCCGCTCCCAAAGTAATCACCAGTCCTGTATGTCATGTGTATCCTGTGGGAGCGGGCGTGCCCGGGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGTCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCg                                    <  5:280476/149‑1 (MQ=38)
                                                                                                                tCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCg                              >  8:118712/1‑149 (MQ=255)
                                                                                                                  ccACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGcc                            >  3:239903/1‑149 (MQ=25)
                                                                                                                    acaGGTATCACATGACCTCCAGGTCTGGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTGGCCGGCATCGCCTc                          >  3:182081/1‑149 (MQ=25)
                                                                                                                              ccAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCAAGATTCGCGTACGAATACGGTCGATCTTGGCCGGCATAGCGTCCAGCGCATa                 >  6:253942/1‑148 (MQ=255)
                                                                                                                                aGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAg              >  5:154504/1‑149 (MQ=255)
                                                                                                                                       gAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGTCATCGCCTCCAGCGCATACAGCTTGccc       <  5:253942/149‑1 (MQ=255)
                                                                                                                                       gAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGccc       <  3:244536/149‑1 (MQ=255)
                                                                                                                                             aTGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCAc  >  2:156421/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
AACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCAC  >  NC_002947/1013107‑1013395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑