Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,076,738 G→C 11.3% Q39H (CAG→CAC)  mreC → cell shape‑determining protein MreC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,076,7380GC11.3% 115.0 / 6.0 44Q39H (CAG→CAC) mreCcell shape‑determining protein MreC
Reads supporting (aligned to +/- strand):  ref base G (18/21);  new base C (2/3);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01

CTTCGCTGGGCGTTCGCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGC  >  NC_002947/1076593‑1076880
                                                                                                                                                  |                                                                                                                                              
cTTCGCTGGGCGTTCGCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAg                                                                                                                                                <  8:44694/146‑1 (MQ=255)
cTTCGCTGGGCGTTCGCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAg                                                                                                                                                >  7:44694/1‑146 (MQ=255)
           gTTCGCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAgg                                                                                                                                  >  5:256424/1‑149 (MQ=255)
            ttCGCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGgt                                                                                                                                 <  1:46270/149‑1 (MQ=255)
            ttCGCCGGCTCGTTCTGGTGGTGATGTCGGTCGCGGTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAAGCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGgt                                                                                                                                 <  7:2562/149‑1 (MQ=255)
               gCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGtgtg                                                                                                                              >  1:282371/1‑149 (MQ=255)
               gCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCtgtg                                                                                                                              >  6:311313/1‑149 (MQ=255)
                ccTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCTGTgg                                                                                                                             <  2:100103/149‑1 (MQ=255)
                ccTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCTGTgg                                                                                                                             <  5:311313/149‑1 (MQ=255)
                   gCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTggccg                                                                                                                          <  6:41107/149‑1 (MQ=255)
                       gTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTggccggc                                                                                                                        <  6:256424/147‑1 (MQ=255)
                        ttCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGgtgt                                                                                                                               <  3:266865/139‑1 (MQ=255)
                        ttCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGgtgt                                                                                                                               >  4:266865/1‑139 (MQ=255)
                              tggtgATGTCGGTCGCGTTGATGGTTGTCGACGCGTCGCTTCGACGTGCTCAAACCCTTGCGCAGCCAGATGGGCCTGGTGCTGATGCAGTCGTACTGGATAACCCATTTGCAACAGCGTGCATGGCAAGGTGTGGCCGGCCAGTTCg                                                                                                                 >  1:48854/1‑148 (MQ=255)
                               ggtgATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTTTGGCCGGCCAGTTCggaa                                                                                                              >  8:55390/1‑147 (MQ=255)
                                gtgATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTa                                                                                                              <  3:116138/148‑1 (MQ=255)
                                 tgATGGCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGc                                                                                                            <  3:34892/149‑1 (MQ=255)
                                  gATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTggccgg                                                                                                                         >  4:160579/1‑135 (MQ=255)
                                  gATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTggccgg                                                                                                                         <  3:160579/135‑1 (MQ=255)
                                      tcggtcgCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGCGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCAc                                                                                                       <  3:113490/149‑1 (MQ=255)
                                       cggtcgCGTTGTTGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGTTGGGCCTGGTGCTGATGGAGTCGTACTTGATCACCGATTTGCCAGAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCAc                                                                                                       <  2:48854/148‑1 (MQ=255)
                                                  aTGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGc                                                                                           >  2:9439/1‑149 (MQ=255)
                                                   tGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGcc                                                                                          <  3:115620/149‑1 (MQ=255)
                                                    ggTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCTGTgg                                                                                                                             <  2:80725/113‑1 (MQ=255)
                                                    ggTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGACCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCTGTgg                                                                                                                             >  1:80725/1‑113 (MQ=255)
                                                            ccggg‑cggTTCGCCGTTCTCAAAACCGTGCGCAGCCAGAAGGGCCTGGTGCTGATGGAGTCGTACTTGATCCCCGATTTGCCACAGCGTGCCTTGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACgag                                                                                 <  7:55390/145‑1 (MQ=255)
                                                                          cGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGAcc                                                                     >  3:204798/1‑148 (MQ=255)
                                                                               tCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGcc                                                               >  1:287279/1‑149 (MQ=255)
                                                                                  aaCCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCctg                                                            <  2:287279/149‑1 (MQ=255)
                                                                                  aaCCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCctg                                                            <  6:168385/149‑1 (MQ=255)
                                                                                               ccAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCACGACCGAAGCCCTGCTGCTGCAGGGgc                                               <  1:9439/149‑1 (MQ=255)
                                                                                                        ccTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGGCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAg                                       >  1:262541/1‑148 (MQ=255)
                                                                                                               cTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAAAGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTggc                               >  5:200523/1‑149 (MQ=255)
                                                                                                                      aGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTg                        <  5:86308/149‑1 (MQ=255)
                                                                                                                       gTCGTACTGGATCACCGATTTGCGACAGCGTGCCTTGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGa                       <  8:123246/149‑1 (MQ=255)
                                                                                                                          gTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCg                    <  2:282371/149‑1 (MQ=255)
                                                                                                                           tACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGAcc                     >  5:132010/1‑147 (MQ=255)
                                                                                                                                gATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAg               >  1:132962/1‑148 (MQ=255)
                                                                                                                                gATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTCCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAg               >  2:138613/1‑148 (MQ=255)
                                                                                                                                     ccGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTg         >  6:281095/1‑149 (MQ=255)
                                                                                                                                     ccGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTg         >  2:193251/1‑149 (MQ=255)
                                                                                                                                             ccACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAg                                                                              <  6:181714/72‑1 (MQ=255)
                                                                                                                                              cacaGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGc                                                                >  7:144143/1‑85 (MQ=255)
                                                                                                                                              cacaGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGc                                                                <  8:144143/85‑1 (MQ=255)
                                                                                                                                                 aGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTgc  <  6:170054/144‑1 (MQ=255)
                                                                                                                                                 aGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTgc  >  5:170054/1‑144 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CTTCGCTGGGCGTTCGCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGC  >  NC_002947/1076593‑1076880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑