Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,076,740	G→T	11.3%	R40L (CGT→CTT)	mreC →	cell shape‑determining protein MreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,076,740	0	G	T	11.3%	119.2 / 6.1	44	R40L (CGT→CTT)	mreC	cell shape‑determining protein MreC
Reads supporting (aligned to +/- strand):  ref base G (18/21);  new base T (2/3);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

GTTCGCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTG  >  NC_002947/1076604‑1076888                                                                                                                                          |                                                                                                                                                     gTTCGCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAgg                                                                                                                                          >  5:256424/1‑149 (MQ=255)  ttCGCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGgt                                                                                                                                         <  1:46270/149‑1 (MQ=255)  ttCGCCGGCTCGTTCTGGTGGTGATGTCGGTCGCGGTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAAGCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGgt                                                                                                                                         <  7:2562/149‑1 (MQ=255)     gCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGtgtg                                                                                                                                      >  1:282371/1‑149 (MQ=255)     gCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCtgtg                                                                                                                                      >  6:311313/1‑149 (MQ=255)      ccTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCTGTgg                                                                                                                                     <  2:100103/149‑1 (MQ=255)      ccTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCTGTgg                                                                                                                                     <  5:311313/149‑1 (MQ=255)         gCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTggccg                                                                                                                                  <  6:41107/149‑1 (MQ=255)             gTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTggccggc                                                                                                                                <  6:256424/147‑1 (MQ=255)              ttCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGgtgt                                                                                                                                       >  4:266865/1‑139 (MQ=255)              ttCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGgtgt                                                                                                                                       <  3:266865/139‑1 (MQ=255)                    tggtgATGTCGGTCGCGTTGATGGTTGTCGACGCGTCGCTTCGACGTGCTCAAACCCTTGCGCAGCCAGATGGGCCTGGTGCTGATGCAGTCGTACTGGATAACCCATTTGCAACAGCGTGCATGGCAAGGTGTGGCCGGCCAGTTCg                                                                                                                         >  1:48854/1‑148 (MQ=255)                     ggtgATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTTTGGCCGGCCAGTTCggaa                                                                                                                      >  8:55390/1‑147 (MQ=255)                      gtgATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTa                                                                                                                      <  3:116138/148‑1 (MQ=255)                       tgATGGCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGc                                                                                                                    <  3:34892/149‑1 (MQ=255)                        gATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTggccgg                                                                                                                                 <  3:160579/135‑1 (MQ=255)                        gATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTggccgg                                                                                                                                 >  4:160579/1‑135 (MQ=255)                            tcggtcgCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGCGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCAc                                                                                                               <  3:113490/149‑1 (MQ=255)                             cggtcgCGTTGTTGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGTTGGGCCTGGTGCTGATGGAGTCGTACTTGATCACCGATTTGCCAGAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCAc                                                                                                               <  2:48854/148‑1 (MQ=255)                                        aTGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGc                                                                                                   >  2:9439/1‑149 (MQ=255)                                         tGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGcc                                                                                                  <  3:115620/149‑1 (MQ=255)                                          ggTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCTGTgg                                                                                                                                     <  2:80725/113‑1 (MQ=255)                                          ggTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGACCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCTGTgg                                                                                                                                     >  1:80725/1‑113 (MQ=255)                                                  ccggg‑cggTTCGCCGTTCTCAAAACCGTGCGCAGCCAGAAGGGCCTGGTGCTGATGGAGTCGTACTTGATCCCCGATTTGCCACAGCGTGCCTTGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACgag                                                                                         <  7:55390/145‑1 (MQ=255)                                                                cGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGAcc                                                                             >  3:204798/1‑148 (MQ=255)                                                                     tCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGcc                                                                       >  1:287279/1‑149 (MQ=255)                                                                        aaCCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCctg                                                                    <  2:287279/149‑1 (MQ=255)                                                                        aaCCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCctg                                                                    <  6:168385/149‑1 (MQ=255)                                                                                     ccAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCACGACCGAAGCCCTGCTGCTGCAGGGgc                                                       <  1:9439/149‑1 (MQ=255)                                                                                              ccTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGGCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAg                                               >  1:262541/1‑148 (MQ=255)                                                                                                     cTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAAAGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTggc                                       >  5:200523/1‑149 (MQ=255)                                                                                                            aGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTg                                <  5:86308/149‑1 (MQ=255)                                                                                                             gTCGTACTGGATCACCGATTTGCGACAGCGTGCCTTGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGa                               <  8:123246/149‑1 (MQ=255)                                                                                                                gTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCg                            <  2:282371/149‑1 (MQ=255)                                                                                                                 tACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGAcc                             >  5:132010/1‑147 (MQ=255)                                                                                                                      gATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAg                       >  1:132962/1‑148 (MQ=255)                                                                                                                      gATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTCCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAg                       >  2:138613/1‑148 (MQ=255)                                                                                                                           ccGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTg                 >  2:193251/1‑149 (MQ=255)                                                                                                                           ccGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTg                 >  6:281095/1‑149 (MQ=255)                                                                                                                                   ccACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAg                                                                                      <  6:181714/72‑1 (MQ=255)                                                                                                                                    cacaGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGc                                                                        >  7:144143/1‑85 (MQ=255)                                                                                                                                    cacaGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGc                                                                        <  8:144143/85‑1 (MQ=255)                                                                                                                                       aGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTgc          <  6:170054/144‑1 (MQ=255)                                                                                                                                       aGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTgc          >  5:170054/1‑144 (MQ=255)                                                                                                                                          gTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTg  <  4:204798/149‑1 (MQ=255)                                                                                                                                          |                                                                                                                                                     GTTCGCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTG  >  NC_002947/1076604‑1076888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑