Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,076,745 T→A 10.4% W42R (TGG→AGG)  mreC → cell shape‑determining protein MreC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,076,7450TA10.4% 124.2 / 5.9 48W42R (TGG→AGG) mreCcell shape‑determining protein MreC
Reads supporting (aligned to +/- strand):  ref base T (19/24);  new base A (2/3);  total (21/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.89e-01

GTTCGCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTC  >  NC_002947/1076604‑1076891
                                                                                                                                              |                                                                                                                                                  
gTTCGCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAgg                                                                                                                                             >  5:256424/1‑149 (MQ=255)
 ttCGCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGgt                                                                                                                                            <  1:46270/149‑1 (MQ=255)
 ttCGCCGGCTCGTTCTGGTGGTGATGTCGGTCGCGGTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAAGCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGgt                                                                                                                                            <  7:2562/149‑1 (MQ=255)
    gCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGtgtg                                                                                                                                         >  1:282371/1‑149 (MQ=255)
    gCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCtgtg                                                                                                                                         >  6:311313/1‑149 (MQ=255)
     ccTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCTGTgg                                                                                                                                        <  5:311313/149‑1 (MQ=255)
     ccTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCTGTgg                                                                                                                                        <  2:100103/149‑1 (MQ=255)
        gCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTggccg                                                                                                                                     <  6:41107/149‑1 (MQ=255)
            gTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTggccggc                                                                                                                                   <  6:256424/147‑1 (MQ=255)
             ttCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGgtgt                                                                                                                                          >  4:266865/1‑139 (MQ=255)
             ttCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGgtgt                                                                                                                                          <  3:266865/139‑1 (MQ=255)
                   tggtgATGTCGGTCGCGTTGATGGTTGTCGACGCGTCGCTTCGACGTGCTCAAACCCTTGCGCAGCCAGATGGGCCTGGTGCTGATGCAGTCGTACTGGATAACCCATTTGCAACAGCGTGCATGGCAAGGTGTGGCCGGCCAGTTCg                                                                                                                            >  1:48854/1‑148 (MQ=255)
                    ggtgATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTTTGGCCGGCCAGTTCggaa                                                                                                                         >  8:55390/1‑147 (MQ=255)
                     gtgATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTa                                                                                                                         <  3:116138/148‑1 (MQ=255)
                      tgATGGCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGc                                                                                                                       <  3:34892/149‑1 (MQ=255)
                       gATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTggccgg                                                                                                                                    <  3:160579/135‑1 (MQ=255)
                       gATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTggccgg                                                                                                                                    >  4:160579/1‑135 (MQ=255)
                           tcggtcgCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGCGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCAc                                                                                                                  <  3:113490/149‑1 (MQ=255)
                            cggtcgCGTTGTTGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGTTGGGCCTGGTGCTGATGGAGTCGTACTTGATCACCGATTTGCCAGAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCAc                                                                                                                  <  2:48854/148‑1 (MQ=255)
                                       aTGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGc                                                                                                      >  2:9439/1‑149 (MQ=255)
                                        tGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGcc                                                                                                     <  3:115620/149‑1 (MQ=255)
                                         ggTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCTGTgg                                                                                                                                        <  2:80725/113‑1 (MQ=255)
                                         ggTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGACCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCTGTgg                                                                                                                                        >  1:80725/1‑113 (MQ=255)
                                                 ccggg‑cggTTCGCCGTTCTCAAAACCGTGCGCAGCCAGAAGGGCCTGGTGCTGATGGAGTCGTACTTGATCCCCGATTTGCCACAGCGTGCCTTGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACgag                                                                                            <  7:55390/145‑1 (MQ=255)
                                                               cGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGAcc                                                                                >  3:204798/1‑148 (MQ=255)
                                                                    tCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGcc                                                                          >  1:287279/1‑149 (MQ=255)
                                                                       aaCCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCctg                                                                       <  2:287279/149‑1 (MQ=255)
                                                                       aaCCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCctg                                                                       <  6:168385/149‑1 (MQ=255)
                                                                                    ccAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCACGACCGAAGCCCTGCTGCTGCAGGGgc                                                          <  1:9439/149‑1 (MQ=255)
                                                                                             ccTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGGCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAg                                                  >  1:262541/1‑148 (MQ=255)
                                                                                                    cTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAAAGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTggc                                          >  5:200523/1‑149 (MQ=255)
                                                                                                           aGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTg                                   <  5:86308/149‑1 (MQ=255)
                                                                                                            gTCGTACTGGATCACCGATTTGCGACAGCGTGCCTTGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGa                                  <  8:123246/149‑1 (MQ=255)
                                                                                                               gTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCg                               <  2:282371/149‑1 (MQ=255)
                                                                                                                tACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGAcc                                >  5:132010/1‑147 (MQ=255)
                                                                                                                     gATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAg                          >  1:132962/1‑148 (MQ=255)
                                                                                                                     gATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTCCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAg                          >  2:138613/1‑148 (MQ=255)
                                                                                                                          ccGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTg                    >  6:281095/1‑149 (MQ=255)
                                                                                                                          ccGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTg                    >  2:193251/1‑149 (MQ=255)
                                                                                                                                  ccACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAg                                                                                         <  6:181714/72‑1 (MQ=255)
                                                                                                                                   cacaGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGc                                                                           >  7:144143/1‑85 (MQ=255)
                                                                                                                                   cacaGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGc                                                                           <  8:144143/85‑1 (MQ=255)
                                                                                                                                      aGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTgc             <  6:170054/144‑1 (MQ=255)
                                                                                                                                      aGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTgc             >  5:170054/1‑144 (MQ=255)
                                                                                                                                         gTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTg     <  4:204798/149‑1 (MQ=255)
                                                                                                                                            ccTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTg                    >  7:291524/1‑131 (MQ=255)
                                                                                                                                            ccTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTg                    <  8:291524/131‑1 (MQ=255)
                                                                                                                                            ccTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTc  <  6:132010/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
GTTCGCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTC  >  NC_002947/1076604‑1076891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑