Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	160,518	G→C	5.7%	A63G (GCA→GGA) ‡	PP_0150 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	160,518	0	G	C	5.7%	234.0 / 3.4	70	A63G (GCA→GGA) ‡	PP_0150	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (33/33);  new base C (2/2);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01

AGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGGC  >  NC_002947/160370‑160660                                                                                                                                                       |                                                                                                                                               aGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCTCCGTTGCTGGCGACGAAGCTTg                                                                                                                                                <  2:224406/149‑1 (MQ=255)  ggCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGc                                                                                                                                               <  5:63361/149‑1 (MQ=255)  ggCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGc                                                                                                                                               <  3:293033/149‑1 (MQ=255)  ggCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGc                                                                                                                                               <  5:79177/149‑1 (MQ=255)         acgcGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGcgtcg                                                                                                                                        >  6:333438/1‑149 (MQ=255)             gcTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGcgtcgtcgt                                                                                                                                    >  8:79338/1‑149 (MQ=255)                 cacaGTGCGAGTATGGCTTCAGCCAATACCTTGTCGCTGGCGGCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGACCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTg                                                                                                                                   <  1:253989/148‑1 (MQ=255)                 cacaGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTgg                                                                                                                                  <  3:40061/147‑1 (MQ=255)                     gTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTcg                                                                                                                            >  5:181449/1‑149 (MQ=255)                     gTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTcg                                                                                                                            >  4:145804/1‑149 (MQ=255)                      tGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCa                                                                                                                                              >  5:57466/1‑130 (MQ=255)                      tGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCa                                                                                                                                              <  6:57466/130‑1 (MQ=255)                      tGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTcgc                                                                                                                           <  5:64059/149‑1 (MQ=255)                          aGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGcac                                                                                                                       <  6:135844/149‑1 (MQ=255)                                gCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGAGCTTCGCGCACCACttt                                                                                                                 >  6:201238/1‑149 (MQ=255)                                 cTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGGTGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTg                                                                                                                >  7:146187/1‑149 (MQ=255)                                  ttCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTg                                                                                                                >  3:289719/1‑148 (MQ=255)                                        cAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTcgc                                                                                                         <  2:256316/149‑1 (MQ=255)                                        cAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTcgc                                                                                                         <  6:181449/149‑1 (MQ=255)                                         aaTACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGGTGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTcgcg                                                                                                        <  8:146187/149‑1 (MQ=255)                                         aaTACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTcgcg                                                                                                        <  3:145804/149‑1 (MQ=255)                                               tGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATc                                                                                                    >  6:77954/1‑147 (MQ=255)                                                 gTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAg                                                                                                 <  5:333438/148‑1 (MQ=255)                                                      tGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAggtggtg                                                                                           >  6:161854/1‑149 (MQ=255)                                                           ccgcGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTc                                                                                      <  5:161854/149‑1 (MQ=255)                                                           ccgcGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTc                                                                                      <  6:108197/149‑1 (MQ=255)                                                            cgcgcgCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGACGCTGCAAGcttcgtcgc                                                                                                                                    <  1:16269/102‑2 (MQ=255)                                                            cgcgcgCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGACGCTGCAAGcttcgtcgc                                                                                                                                    >  2:16269/1‑101 (MQ=255)                                                             gcgcgcTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCt                                                                                     >  5:165062/1‑148 (MQ=255)                                                               gcgcTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAg                                                                                  <  7:25765/149‑1 (MQ=255)                                                                 gcTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGACGCTGCAAGcttcgtc                                                                                                                                      >  5:19278/1‑95 (MQ=255)                                                                 gcTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGACGCTGCAAGcttcgtc                                                                                                                                      <  6:19278/95‑1 (MQ=255)                                                                 gcTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATAGTGGTGGTGGTGTCTGAGGt                                                                                >  8:256140/1‑149 (MQ=255)                                                                  cTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTg                                                                               <  4:289719/149‑1 (MQ=255)                                                                      cGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAg                                                                            <  4:134901/148‑1 (MQ=255)                                                                      cGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGt                                                                           <  3:190061/149‑1 (MQ=255)                                                                            tcgctcgcGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAggt                                                                                               >  6:87567/1‑123 (MQ=255)                                                                            tcgctcgcGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAggt                                                                                               <  5:87567/123‑1 (MQ=255)                                                                              gctcgcGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGaa                                                                   >  7:78041/1‑149 (MQ=255)                                                                                  gcgcAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTg                                                                                                                                   <  7:86616/81‑1 (MQ=255)                                                                                  gcgcAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTg                                                                                                                                   >  8:86616/1‑81 (MQ=255)                                                                                        ggTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGa                                                                                   <  7:99333/123‑1 (MQ=255)                                                                                        ggTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGa                                                                                   >  8:99333/1‑123 (MQ=255)                                                                                            ttGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGgcacg                                                     >  2:294077/1‑149 (MQ=255)                                                                                              gAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGgcacgca                                                   >  3:71954/1‑149 (MQ=255)                                                                                               aaGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGGGAAGTCGATCGAACGGCCAAAGgcacgcac                                                  >  8:126955/1‑149 (MQ=255)                                                                                                 ggCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACga                                                >  6:81456/1‑149 (MQ=255)                                                                                                   ccg‑‑ccTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACgatg                                              <  6:165062/149‑1 (MQ=255)                                                                                                    cg‑‑ccTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACga                                                <  5:208034/146‑1 (MQ=255)                                                                                                    cg‑‑ccTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACga                                                >  6:208034/1‑146 (MQ=255)                                                                                                     g‑‑ccTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACgatgat                                            <  8:78041/149‑1 (MQ=255)                                                                                                        ccTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGAGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGatgatg                                           <  5:201238/149‑1 (MQ=255)                                                                                                         cTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGt                                          >  4:303045/1‑149 (MQ=255)                                                                                                                 gTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCtggtg                                  >  7:202922/1‑149 (MQ=255)                                                                                                                  tACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCtggtgg                                 >  4:306187/1‑149 (MQ=255)                                                                                                                     gccgcGGATGTCAACGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCCACAATTTGGAGTCGAGGATAGAGGTGGTTGTTTGTGAGGTGTATTAGAAAAAACAGCCAAAAGCACGAACCAAGATGTTGCAGGTGGcca                              >  8:108052/1‑149 (MQ=255)                                                                                                                         cgGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAg                          <  3:306187/149‑1 (MQ=255)                                                                                                                          ggATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAgg                         <  4:71954/149‑1 (MQ=255)                                                                                                                                cACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGGTCGAGGTGGTGGGGTGGGAGGTGAAGTCGATCGCACGGCCAAAGGCACGCACGATGATGTTTCTGGTGGCCCCCAgagatcgg                   >  2:319951/1‑142 (MQ=255)                                                                                                                                cACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGccacca                           <  1:319951/141‑1 (MQ=255)                                                                                                                                 aCCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCtgtg                  >  3:26333/1‑149 (MQ=255)                                                                                                                                 aCCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCtgtg                  >  4:298975/1‑149 (MQ=255)                                                                                                                                      tGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTgg             <  8:202922/149‑1 (MQ=255)                                                                                                                                         ccacgacgaAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGc           >  5:333474/4‑148 (MQ=255)                                                                                                                                             gacgaAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGt      >  6:241502/1‑149 (MQ=255)                                                                                                                                              acgaAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTAGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTc     >  8:268551/1‑149 (MQ=255)                                                                                                                                               cgaAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCg    <  5:241502/149‑1 (MQ=255)                                                                                                                                                gaAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCgg   >  1:22148/1‑149 (MQ=255)                                                                                                                                                 aaGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGGc  <  5:81456/149‑1 (MQ=255)                                                                                                                                                     ttGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCtg                    <  7:87071/127‑1 (MQ=255)                                                                                                                                                     ttGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCtg                    >  8:87071/1‑127 (MQ=255)                                                                                                                                                       |                                                                                                                                               AGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCG‑‑CCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAACGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGGC  >  NC_002947/160370‑160660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑