Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,076,752 G→C 11.6% G44A (GGT→GCT)  mreC → cell shape‑determining protein MreC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,076,7520GC11.6% 114.3 / 6.7 43G44A (GGT→GCT) mreCcell shape‑determining protein MreC
Reads supporting (aligned to +/- strand):  ref base G (17/21);  new base C (2/3);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

GTTCGCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCG  >  NC_002947/1076604‑1076900
                                                                                                                                                     |                                                                                                                                                    
gTTCGCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAgg                                                                                                                                                      >  5:256424/1‑149 (MQ=255)
 ttCGCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGgt                                                                                                                                                     <  1:46270/149‑1 (MQ=255)
 ttCGCCGGCTCGTTCTGGTGGTGATGTCGGTCGCGGTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAAGCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGgt                                                                                                                                                     <  7:2562/149‑1 (MQ=255)
    gCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGtgtg                                                                                                                                                  >  1:282371/1‑149 (MQ=255)
    gCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCtgtg                                                                                                                                                  >  6:311313/1‑149 (MQ=255)
     ccTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCTGTgg                                                                                                                                                 <  5:311313/149‑1 (MQ=255)
     ccTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCTGTgg                                                                                                                                                 <  2:100103/149‑1 (MQ=255)
        gCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTggccg                                                                                                                                              <  6:41107/149‑1 (MQ=255)
            gTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTggccggc                                                                                                                                            <  6:256424/147‑1 (MQ=255)
             ttCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGgtgt                                                                                                                                                   >  4:266865/1‑139 (MQ=255)
             ttCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGgtgt                                                                                                                                                   <  3:266865/139‑1 (MQ=255)
                   tggtgATGTCGGTCGCGTTGATGGTTGTCGACGCGTCGCTTCGACGTGCTCAAACCCTTGCGCAGCCAGATGGGCCTGGTGCTGATGCAGTCGTACTGGATAACCCATTTGCAACAGCGTGCATGGCAAGGTGTGGCCGGCCAGTTCg                                                                                                                                     >  1:48854/1‑148 (MQ=255)
                    ggtgATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTTTGGCCGGCCAGTTCggaa                                                                                                                                  >  8:55390/1‑147 (MQ=255)
                     gtgATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTa                                                                                                                                  <  3:116138/148‑1 (MQ=255)
                      tgATGGCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGc                                                                                                                                <  3:34892/149‑1 (MQ=255)
                       gATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTggccgg                                                                                                                                             <  3:160579/135‑1 (MQ=255)
                       gATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTggccgg                                                                                                                                             >  4:160579/1‑135 (MQ=255)
                           tcggtcgCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGCGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCAc                                                                                                                           <  3:113490/149‑1 (MQ=255)
                            cggtcgCGTTGTTGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGTTGGGCCTGGTGCTGATGGAGTCGTACTTGATCACCGATTTGCCAGAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCAc                                                                                                                           <  2:48854/148‑1 (MQ=255)
                                       aTGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGc                                                                                                               >  2:9439/1‑149 (MQ=255)
                                        tGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGcc                                                                                                              <  3:115620/149‑1 (MQ=255)
                                         ggTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCTGTgg                                                                                                                                                 <  2:80725/113‑1 (MQ=255)
                                         ggTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGACCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACACCTTGCCAGGCACGCTGTgg                                                                                                                                                 >  1:80725/1‑113 (MQ=255)
                                                 ccggg‑cggTTCGCCGTTCTCAAAACCGTGCGCAGCCAGAAGGGCCTGGTGCTGATGGAGTCGTACTTGATCCCCGATTTGCCACAGCGTGCCTTGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACgag                                                                                                     <  7:55390/145‑1 (MQ=255)
                                                               cGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGAcc                                                                                         >  3:204798/1‑148 (MQ=255)
                                                                    tCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGcc                                                                                   >  1:287279/1‑149 (MQ=255)
                                                                       aaCCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCctg                                                                                <  2:287279/149‑1 (MQ=255)
                                                                       aaCCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCctg                                                                                <  6:168385/149‑1 (MQ=255)
                                                                                    ccAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCACGACCGAAGCCCTGCTGCTGCAGGGgc                                                                   <  1:9439/149‑1 (MQ=255)
                                                                                             ccTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGGCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAg                                                           >  1:262541/1‑148 (MQ=255)
                                                                                                    cTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAAAGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTggc                                                   >  5:200523/1‑149 (MQ=255)
                                                                                                           aGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTg                                            <  5:86308/149‑1 (MQ=255)
                                                                                                            gTCGTACTGGATCACCGATTTGCGACAGCGTGCCTTGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGa                                           <  8:123246/149‑1 (MQ=255)
                                                                                                               gTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCg                                        <  2:282371/149‑1 (MQ=255)
                                                                                                                tACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGAcc                                         >  5:132010/1‑147 (MQ=255)
                                                                                                                     gATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAg                                   >  1:132962/1‑148 (MQ=255)
                                                                                                                     gATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTCCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAg                                   >  2:138613/1‑148 (MQ=255)
                                                                                                                          ccGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTg                             >  6:281095/1‑149 (MQ=255)
                                                                                                                          ccGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTg                             >  2:193251/1‑149 (MQ=255)
                                                                                                                                  ccACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAg                                                                                                  <  6:181714/72‑1 (MQ=255)
                                                                                                                                   cacaGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGc                                                                                    >  7:144143/1‑85 (MQ=255)
                                                                                                                                   cacaGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGc                                                                                    <  8:144143/85‑1 (MQ=255)
                                                                                                                                      aGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTgc                      <  6:170054/144‑1 (MQ=255)
                                                                                                                                      aGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTgc                      >  5:170054/1‑144 (MQ=255)
                                                                                                                                         gTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTg              <  4:204798/149‑1 (MQ=255)
                                                                                                                                            ccTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTg                             >  7:291524/1‑131 (MQ=255)
                                                                                                                                            ccTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTg                             <  8:291524/131‑1 (MQ=255)
                                                                                                                                            ccTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTc           <  6:132010/149‑1 (MQ=255)
                                                                                                                                                     gtgtGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCg  <  5:281095/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTTCGCCTGCTCGTTCTGGTGGTGATGTCGGTCGCGTTGATGGTTGTCGACGCG‑CGCTTCGACGTGCTCAAACCCGTGCGCAGCCAGATGGGCCTGGTGCTGATGGAGTCGTACTGGATCACCGATTTGCCACAGCGTGCCTGGCAAGGTGTGGCCGGCCAGTTCGGTAGCCGCACCGAGCTGATTGCCGAAAACGAGAAGCTCAAGACCGAAGCCCTGCTGCTGCAGGGGCGCCTGCAGAAGCTGGCGGCCCTGACCGAGCAGAACGTGCGGCTGCGCGAGTTGCTCAACTCTTCG  >  NC_002947/1076604‑1076900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑