Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,120,471 T→A 9.5% intergenic (‑213/‑89) pepA ← / → PP_0982 cytosol aminopeptidase/YjgP/YjgQ family permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,120,4710TA 9.5% 174.2 / 6.3 63intergenic (‑213/‑89)pepA/PP_0982cytosol aminopeptidase/YjgP/YjgQ family permease
Reads supporting (aligned to +/- strand):  ref base T (29/27);  new base A (3/3);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCGTCGTCGCGCACCAGCGGAAAAGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCTTGAGCGCCGTCAG  >  NC_002947/1120323‑1120615
                                                                                                                                                    |                                                                                                                                                
ccGCGTCGTCGCGCACCAGCGGAAAAGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCttt                                                                                                                                                  <  8:20820/149‑1 (MQ=255)
  gcgTCGTCGCGCACCAGCGGAAAAGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGTGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTgg                                                                                                                                                <  7:89173/149‑1 (MQ=255)
        tcgCGCACCAGCGGAAAAGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAAcc                                                                                                                                          <  8:153365/149‑1 (MQ=255)
          gcgcACCAGCGGAAAAGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCgg                                                                                                                                        <  8:112325/149‑1 (MQ=255)
             cACCAGCGGAAAAGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCAt                                                                                                                                     <  3:204664/149‑1 (MQ=255)
               ccAGCGGAAAAGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTg                                                                                                                                   <  1:290952/149‑1 (MQ=255)
                cAGCGGAAAAGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTgg                                                                                                                                  >  7:75426/1‑149 (MQ=255)
                 aGCGGAAAAGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTg                                                                                                                                                 <  8:72028/133‑1 (MQ=255)
                 aGCGGAAAAGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTg                                                                                                                                                 >  7:72028/1‑133 (MQ=255)
                    ggAAAAGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCtt                                                                                                                              >  6:322193/1‑149 (MQ=255)
                              ccAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGAt                                                                                                                          >  1:312309/1‑143 (MQ=255)
                              ccAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGAt                                                                                                                          <  2:312309/143‑1 (MQ=255)
                                      aGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCAtt                                                                                                             <  8:75426/148‑1 (MQ=255)
                                        ccTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGtt                                                                                                          >  2:45525/1‑149 (MQ=255)
                                        ccTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCAAAGGCGAAc                                                                                                                                           <  8:206252/116‑1 (MQ=255)
                                        ccTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCAAAGGCGAAc                                                                                                                                           >  7:206252/1‑116 (MQ=255)
                                              gCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGgcgc                                                                                                    >  2:143725/1‑149 (MQ=255)
                                                aaGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCa                                                                                                  >  5:312866/1‑149 (MQ=255)
                                                  gCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCAtt                                                                                                >  7:6598/1‑149 (MQ=255)
                                                    gTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGc                                                                                              <  1:143725/149‑1 (MQ=255)
                                                       cTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCAt                                                                                            <  5:322193/148‑1 (MQ=255)
                                                         gACAACCCCGATGTGCGCCTTCGTACGTGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCACACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATgg                                                                                          >  6:253210/1‑148 (MQ=255)
                                                          aCAACCCCGATGTTCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTTGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATgg                                                                                          <  6:272617/147‑1 (MQ=255)
                                                            aaCCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGt                                                                                      >  1:38287/1‑149 (MQ=255)
                                                            aaCCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGt                                                                                      >  5:90437/1‑149 (MQ=255)
                                                               cccGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAATGCATTGTTTGGCGCCATTGCATGGCAGTcag                                                                                   >  8:139790/1‑147 (MQ=255)
                                                               cccGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCg                                                                                   >  5:404/1‑149 (MQ=255)
                                                                  gATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGccc                                                                                >  5:80433/1‑149 (MQ=255)
                                                                   aTGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCt                                                                               <  1:45525/149‑1 (MQ=255)
                                                                        cgcCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTCGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACaa                                                                          <  5:172276/149‑1 (MQ=255)
                                                                            ttCGTACGCGGCCAAGTGACAGGGGCGGTCTATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGgc                                                                                                      >  1:26122/1‑117 (MQ=255)
                                                                            ttCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGgc                                                                                                      <  2:26122/117‑1 (MQ=255)
                                                                                     ggCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCt                                                             >  7:275657/1‑149 (MQ=255)
                                                                                      gCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTg                                                            <  6:90437/149‑1 (MQ=255)
                                                                                       ccAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTgg                                                           >  5:2774/1‑149 (MQ=255)
                                                                                        cAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGt                                                          <  2:38287/149‑1 (MQ=255)
                                                                                              aCAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGAt                                                     <  6:80433/148‑1 (MQ=255)
                                                                                                  gggCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGc                                                                                              >  1:195436/1‑103 (MQ=255)
                                                                                                    gCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCttt                                              <  8:275657/149‑1 (MQ=255)
                                                                                                     cGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTc                                             <  6:2774/149‑1 (MQ=255)
                                                                                                          aaTCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTAt                                        <  5:20252/149‑1 (MQ=255)
                                                                                                              acaGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGt                                    <  6:312866/149‑1 (MQ=255)
                                                                                                                  gATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCcg                                <  8:6598/149‑1 (MQ=255)
                                                                                                                  gATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCcg                                >  2:43714/1‑149 (MQ=255)
                                                                                                                        gcgcgcACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGt                          >  3:33911/1‑149 (MQ=255)
                                                                                                                                  tACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCAc                 <  6:404/148‑1 (MQ=255)
                                                                                                                                     atgccggTTCGCCAAAGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCTTg             >  3:28158/6‑149 (MQ=255)
                                                                                                                                     aTGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCtt              >  5:281174/1‑148 (MQ=255)
                                                                                                                                     aTGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCtt              >  8:146378/1‑148 (MQ=255)
                                                                                                                                     aTGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATATGTCCCGCGAGGTCATGTTCAACtt              >  8:211265/1‑148 (MQ=255)
                                                                                                                                      tgccggTTCGCCAAAGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGGCACCTTGa            <  4:28158/145‑1 (MQ=255)
                                                                                                                                      tGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCTTGa            <  7:146378/149‑1 (MQ=255)
                                                                                                                                       ggAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAgg                           >  7:213455/1‑133 (MQ=255)
                                                                                                                                       ggAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAgg                           <  8:213455/133‑1 (MQ=255)
                                                                                                                                       ggAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCtt              <  2:21397/146‑1 (MQ=255)
                                                                                                                                       ggAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCtt              >  1:21397/1‑146 (MQ=255)
                                                                                                                                        gAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGtt                                          >  4:280066/1‑117 (MQ=255)
                                                                                                                                        gAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGtt                                          <  3:280066/117‑1 (MQ=255)
                                                                                                                                        ccggTTCGCCAAAGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCTTGa            <  6:1045/145‑1 (MQ=255)
                                                                                                                                        ccggTTCGCCAAAGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCTTGa            >  5:1045/3‑147 (MQ=255)
                                                                                                                                           gTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCTTGAGCGcc       >  3:87558/1‑149 (MQ=255)
                                                                                                                                            ttCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCTTGAGCGCCg      >  4:185993/1‑149 (MQ=255)
                                                                                                                                               gCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCTTGAGCGCCGt     >  8:113913/1‑147 (MQ=255)
                                                                                                                                                ccTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCTTGAGCGCCGTCAg  <  4:33911/149‑1 (MQ=255)
                                                                                                                                                  tttGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGttt                                                        >  1:77911/1‑93 (MQ=255)
                                                                                                                                                  tttGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGttt                                                        <  2:77911/93‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CCGCGTCGTCGCGCACCAGCGGAAAAGCTGCCAGTTTGAGCCTGTGGCAAGCGTGCTGACAACCCCGATGTGCGCCTTCGTACGCGGCCAAGTGACAGGGGCGGTCAATCACAGGATAATGCGCGCACTTTACATGGAGGTTCGCCTTTGGCGAACCGGCATTGGTCTTGGATGTACTAAGTCATTGTTTGGCGCCATTGCATGGCAGTCCGCCCTGACAACCCAGGAGTGTCTGGTTTGATCGTCTTTCGTTATCTGTCCCGCGAGGTCCTGGTCACCTTGAGCGCCGTCAG  >  NC_002947/1120323‑1120615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑