Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,161,005 G→C 6.8% G273R (GGG→CGG)  gtsC → mannose/glucose ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,161,0050GC 6.8% 177.5 / 3.1 59G273R (GGG→CGG) gtsCmannose/glucose ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (24/31);  new base C (2/2);  total (26/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.64e-01

GATTCGCAACCGATCACCGTAGCCCTGAACAATCTGGTCAATACCAGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCGGATACCCTCAAGGACATCCAGCTGTCGATCAAGGACGGCGAGTTCCTGATCCTGGTGGGC  >  NC_002947/1160858‑1161151
                                                                                                                                                   |                                                                                                                                                  
gATTCGCAACCGATCACCGTAGCCCTGAACAATCTGGTCAATACCAGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCgg                                                                                                                                                   <  4:282021/149‑1 (MQ=255)
 aTTCGCAACCGATCACCGTAGCCCTGAACAATCTGGTCAATACCAGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCggg                                                                                                                                                  <  1:217353/149‑1 (MQ=255)
         ccGATCACCGTAGCCCTGAACAATCTGGTCAATACCAGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGAcggc                                                                                                                                          >  6:73905/1‑149 (MQ=255)
         ccGATCACCGTAGCCCTGAACAATCTGGTCAATACCAGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGAcggc                                                                                                                                          <  8:119481/149‑1 (MQ=255)
          cGATCACCGTAGCCCTGAACAATCTGGTCAATACCAGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGAcggcc                                                                                                                                         >  8:143609/1‑149 (MQ=255)
           gctCACCCTAGCCCTGAACAATCTTGGCAATACCAGCACCGGGGCCAAGGAATACAACGTCGACATTGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGAcggccg                                                                                                                                        <  8:124882/147‑1 (MQ=255)
               aCCGTAGCCCTGAACAATCTGGTCAATACCAGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGgcgc                                                                                                                                    <  5:73905/149‑1 (MQ=255)
               aCCGTAGCCCTGAACAATCTGGTCAATACCAGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCCGGCCGTCAGCCcgcg                                                                                                                                     >  3:214240/1‑148 (MQ=255)
                ccGTAGCCCTGAACAATCTGGTCAATACCAGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCCGGCCGTCAGCCCgcgca                                                                                                                                   <  4:214240/149‑2 (MQ=255)
                    aGCCCTGAACAATCTGGTCAATACCAGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTc                                                                                                                                >  7:198392/1‑148 (MQ=255)
                      cccTGAACAATCTGGTCAATACCAGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAg                                                                                                                             >  7:248469/1‑149 (MQ=255)
                        cTGAACAATCTGGTCAATACCAGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAggg                                                                                                                           <  4:226900/149‑1 (MQ=255)
                           aaCAATCTGGTCAATACCAGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGt                                                                                                                         <  8:198392/148‑1 (MQ=255)
                                tCTGGTCAATACCAGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGGCAAGGGGTAAGCCa                                                                                                                   >  7:263701/1‑149 (MQ=255)
                                  tGGTCAATACCAGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATg                                                                                                                 >  5:282761/1‑149 (MQ=255)
                                   cgTCAATACCAGCACCGGGGCCAAGGAATACAACCCCGCCCTGCCCGCAGCGATGATCCCTGGCCTGCCAACCCTGCTGCCCTACGTGGAGGCACGCAAATATATCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCAggg                                                                                                                <  6:242962/148‑1 (MQ=255)
                                    gTCAATACCAGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATg                                                                                                                 <  5:2241/147‑1 (MQ=255)
                                       aaTACCAGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCaa                                                                                                             <  6:238009/148‑1 (MQ=255)
                                            cAGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTc                                                                                                        >  2:317004/1‑148 (MQ=255)
                                             aGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGa                                                                                                      <  8:248469/149‑1 (MQ=255)
                                                aCCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACt                                                                                                   >  2:48439/1‑149 (MQ=255)
                                                  cGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTc                                                                                                 >  6:212030/1‑149 (MQ=255)
                                                  cGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCCGGCCGTCAGCCCgcgca                                                                                                                                   <  5:93154/115‑2 (MQ=255)
                                                  cGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCCGGCCGTCAGCCCgcgca                                                                                                                                   >  6:93154/1‑114 (MQ=255)
                                                   ggggCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCg                                                                                                <  6:282761/149‑1 (MQ=255)
                                                      gCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCaa                                                                                             <  3:49457/149‑1 (MQ=255)
                                                       ccAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCAt                                                                                                                  <  6:198677/127‑1 (MQ=255)
                                                       ccAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCAt                                                                                                                  >  5:198677/1‑127 (MQ=255)
                                                           ggAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGa                                                                                        <  1:317004/149‑1 (MQ=255)
                                                            gAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGaa                                                                                       <  5:212030/149‑1 (MQ=255)
                                                                 cAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAg                                                                                   <  7:143609/148‑1 (MQ=255)
                                                                  aaCGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCTACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCAt                                                                                                                  >  5:132353/1‑116 (MQ=255)
                                                                  aaCGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCAt                                                                                                                  <  6:132353/116‑1 (MQ=255)
                                                                         aCATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACgg                                                                           >  6:169680/1‑148 (MQ=255)
                                                                           aTGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGCCGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAg                                                                        >  8:164417/1‑149 (MQ=255)
                                                                           aTGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCgg                                                                                                                                  >  7:139535/1‑91 (MQ=255)
                                                                           aTGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCgg                                                                                                                                  <  8:139535/91‑1 (MQ=255)
                                                                              gcggcAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGTCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCg                                                                      <  5:169680/148‑1 (MQ=255)
                                                                              gcggcAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCg                                                                      <  1:14753/148‑1 (MQ=255)
                                                                                 gcAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGAcc                                                                                >  4:84969/1‑135 (MQ=255)
                                                                                 gcAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGAcc                                                                                <  3:84969/135‑1 (MQ=255)
                                                                                    gCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGcc                                                               <  8:150808/149‑1 (MQ=255)
                                                                                      gatgatCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCg                                                              >  6:53290/1‑148 (MQ=255)
                                                                                           tCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCGGATAcc                                                        <  3:49136/149‑1 (MQ=255)
                                                                                                       cAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCGGATACCCTCAAGGACATc                                            >  6:79655/1‑149 (MQ=255)
                                                                                                        aaCCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCGGATACCCTCAAGGACATc                                            >  5:45402/1‑148 (MQ=255)
                                                                                                          cccTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCGGATACCCTCAAGGACATCCAg                                         <  5:53290/149‑1 (MQ=255)
                                                                                                                   tCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCGGATACCCTCAAGGACATCCAGCTGTCGAt                                 >  6:133958/1‑148 (MQ=255)
                                                                                                                      atgtggtgGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTTAACAAGACCTACGGCAGCGGCCTGCCGGATACCCTCAAGGACATCCAGCTGt                                     <  2:227992/139‑1 (MQ=255)
                                                                                                                      aCGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGGGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCGGATACCCTAAAGGACATCCAGCTGTcagat                                >  1:227992/1‑142 (MQ=255)
                                                                                                                       cGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCGGATACCCTCAAGGACATCCAGCTGTCGATCAAg                             <  5:79655/148‑1 (MQ=255)
                                                                                                                        gtggtgGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCGGATACCCTCAAGGACATCCAGCTGTCGATCAAGGa                           >  4:72836/1‑149 (MQ=255)
                                                                                                                          ggtggCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCGGATACCCTCAAGGACATCCAGCTGTCGATCAAGGACg                         >  2:35043/1‑149 (MQ=255)
                                                                                                                              gcaggcaAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCGGATACCCTCAAGGACATCCAGCTGTCGATCAAGGACGGCGa                     <  8:219022/149‑1 (MQ=255)
                                                                                                                                    aaaTATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCGGATACCCTCAAGGACATCCAGCTGTCGATCAAGGACGGCGAGTTCCt               >  4:147777/1‑149 (MQ=255)
                                                                                                                                      atatTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCGGATACCCTCAAGGACATCCAGCTGTCGATCAAGGACGGCGAGTTCCTGa             <  3:147777/149‑1 (MQ=255)
                                                                                                                                         tttCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCGGATACCCTCAAGGACATCCAGCTGTCGATCAAGGACGGCGAGTTCCTGATc           <  5:133958/148‑1 (MQ=255)
                                                                                                                                          ttCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCGGATACCCTCAAGGACATCCAGCTGTCGATCAAGGACGGCGAGTTCCt               >  5:123043/1‑143 (MQ=255)
                                                                                                                                          ttCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCGGATACCCTCAAGGACATCCAGCTGTCGATCAAGGACGGCGAGTTCCt               <  6:123043/143‑1 (MQ=255)
                                                                                                                                              tGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGCACAAGACCTACGGCAGCGGCCTGCCGGATACCCTCAAGGACATCCAGCTGTCGATCAAGGACGGCGAGTTCCTGATCCtggtg     <  3:231511/149‑1 (MQ=255)
                                                                                                                                              tGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCGGATACCCTCAAGGACATCCAGCTGTCGATCAAGGACGGCGAGTTCCTGATCCtggtg     <  3:227243/149‑1 (MQ=255)
                                                                                                                                                 gcgGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCGGATACCCTCAAGGACATCCAGCTGTCGATCAAGGACGGCGAGTTCCTGATCCTGGTGGGc  <  1:35043/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATTCGCAACCGATCACCGTAGCCCTGAACAATCTGGTCAATACCAGCACCGGGGCCAAGGAATACAACGTCGACATGGCGGCAGCGATGATCGCTGGCCTGCCAACCCTGCTGGTCTACGTGGTGGCAGGCAAATATTTCGTGCGCGGGCTGACGGCCGGCGCGGTCAAGGGGTAAGCCATGGCAACGCTCGAACTTCGCAATGTGAACAAGACCTACGGCAGCGGCCTGCCGGATACCCTCAAGGACATCCAGCTGTCGATCAAGGACGGCGAGTTCCTGATCCTGGTGGGC  >  NC_002947/1160858‑1161151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑