Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,193,928 T→A 5.6% L23M (TTG→ATG) ‡ uxpA → lipoprotein UxpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,193,9280TA 5.6% 216.8 / 2.3 71L23M (TTG→ATG) ‡uxpAlipoprotein UxpA
Reads supporting (aligned to +/- strand):  ref base T (33/34);  new base A (2/2);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

GGAATTGCTGTCGGATTTCTGCCATCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCGGTCA  >  NC_002947/1193782‑1194072
                                                                                                                                                  |                                                                                                                                                 
ggAATTGCTGTCGGATTTCTGCCATCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCtt                                                                                                                                                  <  3:235798/148‑1 (MQ=255)
 gAATTGCTGTCCGATTTCTGCCATCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCGGGATGGGTATCGGTGCCTTgg                                                                                                                                                <  6:203203/149‑1 (MQ=255)
  aaTTGCTGTCGGATTTCTGCCATCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTgg                                                                                                                                                <  4:189847/148‑1 (MQ=255)
    ttGCTGTCGGATTTCTGCCATCAATCTGTTATGCGAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGTTGGCGGATGCGATGGCGAGCAGTGTGCTCAGGCGCGAAGTGATCGGCTGGATGGGTTTCGGTGCCAAGGCAc                                                                                                                                             <  4:79141/149‑1 (MQ=255)
                ttCTGCCATCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGgc                                                                                                                                  <  6:217803/148‑1 (MQ=255)
                   tGCCATCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCAAGGCACCga                                                                                                                                          >  6:240493/1‑136 (MQ=255)
                   tGCCATCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCAAGGCACCga                                                                                                                                          <  5:240493/137‑2 (MQ=255)
                     ccATCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTg                                                                                                                             >  1:29138/1‑148 (MQ=255)
                       aTCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCt                                                                                                                              >  6:55916/1‑145 (MQ=255)
                       aTCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCt                                                                                                                              <  5:55916/145‑1 (MQ=255)
                       aTCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGcac                                                                                                                          >  2:61821/1‑149 (MQ=255)
                        tCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGca                                                                                                                           >  2:288953/1‑147 (MQ=255)
                         cAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGTCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAg                                                                                                                        >  7:139056/1‑149 (MQ=255)
                          aaaCTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATTGCGGATGCGATGGCCAGCAGAGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGc                                                                                                                       >  1:181493/1‑149 (MQ=255)
                          aaaCTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGc                                                                                                                       >  3:22555/1‑149 (MQ=255)
                           aaCTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGgcgc                                                                                                                                <  6:174402/139‑1 (MQ=255)
                           aaCTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGgcgc                                                                                                                                >  5:174402/1‑139 (MQ=255)
                            aCTGTAATGCCAACGCCGCAACATCCGGGTNCCTGAATGGACGTGGTTTACGAAGGCGGATGCGATGGCGAGCACTGTGCACACGCGCGAAGTGATCCGCTGGATGGGTATCCGTGCCTTGGCACCGCTGCACCGCGCCAGCACAGcca                                                                                                                     >  1:30464/1‑148 (MQ=255)
                              tGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCt                                                                                                                              <  6:151784/138‑1 (MQ=255)
                              tGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCt                                                                                                                              >  5:151784/1‑138 (MQ=255)
                              tGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTc                                                                                                                   >  5:262422/1‑149 (MQ=255)
                                 ggggtcAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCa                                                                                                                <  3:63009/144‑1 (MQ=255)
                                      cAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGTCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGgca                                                                                                           <  8:139056/149‑1 (MQ=255)
                                       aaCGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGgcag                                                                                                          >  4:23017/1‑149 (MQ=255)
                                         cgccgcAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGgcagca                                                                                                        <  6:262422/149‑1 (MQ=255)
                                            cgcAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAg                                                                                                                        <  3:264210/130‑1 (MQ=255)
                                            cgcAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAg                                                                                                                        >  4:264210/1‑130 (MQ=255)
                                              cAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGggcg                                                                                                   >  5:298158/1‑149 (MQ=255)
                                               aaCATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGggcgg                                                                                                  <  3:23017/149‑1 (MQ=255)
                                                aCATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAg                                                                                                                        >  8:281992/1‑126 (MQ=255)
                                                aCATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAg                                                                                                                        <  7:281992/126‑1 (MQ=255)
                                                 cATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGggcggc                                                                                                 >  7:3681/1‑148 (MQ=255)
                                                       ggTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑ccc                                                                                         <  5:141388/149‑1 (MQ=255)
                                                        gTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCa                                                                                        <  4:121784/149‑1 (MQ=255)
                                                           ccTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGc                                                                                      <  7:22036/148‑1 (MQ=255)
                                                             tGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCg                                                                                   >  4:271917/1‑149 (MQ=255)
                                                                   gACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTg                                                                              <  1:288953/148‑1 (MQ=255)
                                                                   gACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGc                                                                             >  5:47040/1‑149 (MQ=255)
                                                                              ggATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGcc                                                                  >  2:273211/1‑149 (MQ=255)
                                                                              ggATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGcc                                                                  >  2:113029/1‑149 (MQ=255)
                                                                              ggATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGcc                                                                  <  6:47040/149‑1 (MQ=255)
                                                                                    cGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATAcc                                                            >  5:62372/1‑149 (MQ=255)
                                                                                        tGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTc                                                         >  7:25308/1‑148 (MQ=255)
                                                                                        tGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTc                                                         >  7:20508/1‑148 (MQ=255)
                                                                                        tGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCGGGAGGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCGCAGCCGTCCCAGGGCCGCAGGGCGGCAATG‑CCCAGCGCGCCTTGCGGTCCGTCGCGGATACCCTCg                                                        >  8:244159/1‑149 (MQ=255)
                                                                                          cGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGAt                                                      <  8:25308/149‑1 (MQ=255)
                                                                                          cGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGAt                                                      <  8:20508/149‑1 (MQ=255)
                                                                                           gATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATg                                                     >  6:18115/1‑149 (MQ=255)
                                                                                                 gAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGc                                                >  7:77703/1‑148 (MQ=255)
                                                                                                         tgCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTgg                                        <  6:161540/148‑1 (MQ=255)
                                                                                                             caGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTc                                                         >  7:141275/1‑127 (MQ=255)
                                                                                                             caGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTc                                                         <  8:141275/127‑1 (MQ=255)
                                                                                                                  gcgAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCggtg                              <  3:271917/149‑1 (MQ=255)
                                                                                                                   cgAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCggtgg                             >  3:275707/1‑149 (MQ=255)
                                                                                                                      aGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTAc                          <  1:273211/149‑1 (MQ=255)
                                                                                                                             ggCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTAc                   >  8:221901/1‑149 (MQ=255)
                                                                                                                                   aTGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGtt             >  1:252834/1‑149 (MQ=255)
                                                                                                                                      ggTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTggg          <  6:298158/149‑1 (MQ=255)
                                                                                                                                      ggTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTgg           <  4:275707/148‑1 (MQ=255)
                                                                                                                                        cagcGGTGCCAAGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGACCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTgggg         >  1:257594/4‑149 (MQ=255)
                                                                                                                                          tCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCg      <  8:77703/149‑1 (MQ=255)
                                                                                                                                           cGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTg                                                                              >  4:293847/1‑76 (MQ=255)
                                                                                                                                           cGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTg                                                                              <  3:293847/76‑1 (MQ=255)
                                                                                                                                            ggTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCgg     <  4:209970/148‑1 (MQ=255)
                                                                                                                                            ggTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCGGt    <  2:252834/149‑1 (MQ=255)
                                                                                                                                             gTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCg      >  2:137974/1‑146 (MQ=255)
                                                                                                                                             gTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCg      <  1:137974/146‑1 (MQ=255)
                                                                                                                                             gTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCGGTc   <  5:18115/149‑1 (MQ=255)
                                                                                                                                              tGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCGGTCa  <  7:244159/149‑1 (MQ=255)
                                                                                                                                              tGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCGGTCa  <  7:221901/149‑1 (MQ=255)
                                                                                                                                                ccTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCg      <  4:254664/143‑1 (MQ=255)
                                                                                                                                                ccTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCg      >  3:254664/1‑143 (MQ=255)
                                                                                                                                                  ttGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGc        <  3:101660/139‑1 (MQ=255)
                                                                                                                                                  ttGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGc        >  4:101660/1‑139 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGAATTGCTGTCGGATTTCTGCCATCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATG‑CCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCGGTCA  >  NC_002947/1193782‑1194072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑