Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,237,657 A→G 13.1% L151P (CTG→CCG) ‡ PP_1078 ← ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,237,6570AG13.1% 92.8 / 5.7 38L151P (CTG→CCG) ‡PP_1078ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (20/13);  new base G (3/2);  total (23/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GGCTTCTTCCTGGTCATGGGTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTGGGTGTTTGGCGATACCAAAG  >  NC_002947/1237509‑1237797
                                                                                                                                                    |                                                                                                                                            
ggCTTCTTCCTGGTCATGGGTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCa                                                                                                                                              <  5:38707/149‑1 (MQ=255)
  cttcttCCTGGTCATGGGTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTCGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAgc                                                                                                                                            >  6:31688/1‑149 (MQ=255)
   ttcttcCTGGTCATGGGTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAgcg                                                                                                                                           >  3:231027/1‑149 (MQ=255)
         cTGGTCATGGGTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCgg                                                                                                                                      <  1:17580/148‑1 (MQ=255)
          tGGTCATGGGTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCggg                                                                                                                                     >  5:174694/1‑148 (MQ=255)
            gTCATGGGTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCggg                                                                                                                                     <  6:174694/146‑1 (MQ=255)
                    tGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGaca                                                                                                                          <  4:150377/149‑1 (MQ=255)
                            aTGGCGCTGGTACCGCGGCTTTTGAGAATGGCGCGAACCTCATGGCGCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTtgctg                                                                                                                  <  5:238751/149‑1 (MQ=255)
                             tGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTtgctgc                                                                                                                 <  4:231027/149‑1 (MQ=255)
                                           cgGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAg                                                                                                   >  7:4836/1‑149 (MQ=255)
                                            ggCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTCGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGc                                                                                                  <  5:31688/149‑1 (MQ=255)
                                              cTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTc                                                                                                <  5:98794/149‑1 (MQ=255)
                                                     gaATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCggg                                                                                         >  3:196854/1‑149 (MQ=255)
                                                        tGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTaa                                                                                      >  1:271431/1‑149 (MQ=255)
                                                            gcgAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCCCGGGCGCTGGCGCCCGAGccgcaa                                                                                                                               <  2:227065/104‑5 (MQ=38)
                                                            gcgAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCCCGGGCGCTGGCGCCCGAGccgcaa                                                                                                                               >  1:227065/1‑100 (MQ=38)
                                                                  cTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGc                                                                                            >  6:307311/1‑133 (MQ=255)
                                                                  cTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAAcgcccgcc                                                                              >  3:132366/1‑147 (MQ=255)
                                                                  cTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATCGCTCGTCAAGCAGCAGCAGTTTCGGCACCGGCGCCAGCGCCCGCGCCAGGGCGCCACGTTGCTGCTGCACGCCGGACAGCTCATGCTGGTAACGCCAGCCCa                                                                            >  2:246115/1‑149 (MQ=255)
                                                                         cTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCccgcc                                                                     >  5:72829/1‑149 (MQ=255)
                                                                              gcgcCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGtt                                                                >  3:50922/1‑149 (MQ=255)
                                                                                    gcgcAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCa                                                          <  4:50922/149‑1 (MQ=255)
                                                                                      gcAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGc                                                        >  1:45671/1‑149 (MQ=255)
                                                                                                  cGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATctct                                            >  1:7530/1‑149 (MQ=255)
                                                                                                         ggAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGa                                     >  4:51019/1‑149 (MQ=255)
                                                                                                           agaATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCATCGATGACg                                   >  6:126153/1‑149 (MQ=255)
                                                                                                            gaATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGAGCAGCTCCAGCATCTCTTCGATGACgg                                  >  6:322273/1‑149 (MQ=255)
                                                                                                                     gTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGc                         <  3:51019/149‑1 (MQ=255)
                                                                                                                         agcagcagcAGTTGCGGCTCGGGCGCCAGCGACCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTgg                     <  4:196854/149‑1 (MQ=255)
                                                                                                                             gcagcagTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCtt                                           <  2:90162/123‑1 (MQ=255)
                                                                                                                              cagcagTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTGGGTGtt                >  8:113912/1‑149 (MQ=255)
                                                                                                                               agcagTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTGGGTGttt               <  6:72829/149‑1 (MQ=255)
                                                                                                                                 cagTTGCTGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCGAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTTGGTGTTTgg             <  2:271431/149‑1 (MQ=255)
                                                                                                                                      gCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGa                                                                   >  4:110579/1‑90 (MQ=255)
                                                                                                                                        ggCCCGGGCGCTGGCGCCCGAGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTGGGTGTTTGGCGATAc       >  8:234507/1‑148 (MQ=255)
                                                                                                                                         gcccGGGCGCTGGCGCCCGAGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTGGGTGTTTGGCGATAcc      >  4:296811/4‑148 (MQ=255)
                                                                                                                                            cGGGCGCTGGCGCCCGAGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTGGGTGTTTGGCGATACCAAAg  <  3:296811/149‑1 (MQ=255)
                                                                                                                                            cGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTGGGTGTTTGGCGATACCAAAg  >  3:48802/1‑149 (MQ=255)
                                                                                                                                               gcgcCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGa                                     >  4:179812/1‑111 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
GGCTTCTTCCTGGTCATGGGTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTGGGTGTTTGGCGATACCAAAG  >  NC_002947/1237509‑1237797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑