| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 170,841 | A→C | 11.8% | intergenic (+22/‑548) | PP_0159 → / → PP_16SA | family III CoA‑transferase/16S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 170,841 | 0 | A | C | 11.8% | 81.1 / 2.3 | 33 | intergenic (+22/‑548) | PP_0159/PP_16SA | family III CoA‑transferase/16S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base A (16/12); new base C (2/2); total (18/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAG > NC_002947/170696‑170985 | gaCGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGATGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGc < 1:311684/149‑1 (MQ=255) aCGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACAGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGc < 2:200270/148‑1 (MQ=255) gCCAGTGGAGTATCGCCGGGCGCCACCACTGATGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCATCAGGGCTc > 1:294611/1‑149 (MQ=255) aGTGGCGTATCGCCGGGCGCCACCACTGCTGGGCGAGCTTACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCGGGCGAGGTACAGCGGCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCTGACGGCCTcccc < 5:265100/149‑1 (MQ=255) aGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGGGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTTATGCTGGGGTGCTCTAGATTAAAAGCGGGGAGGCCGTCAGGCCTcccc < 4:283447/149‑1 (MQ=255) gCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGtttt > 4:240584/1‑148 (MQ=255) gcCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAgcgc > 1:89865/1‑148 (MQ=255) agtgctgGGCGAGAATACCCAAGTGGTGGGGGGGTACGTGTGGGGGCTGTTTGCGGGCTAGGTACCGCGTCTGGGTTTTGGTGGGGTGCTATATATCAAAAGGGGGCAGGCCGTCTGGCCTCCCCGCTTTGTTTTTCAGCGCTATCaaa < 6:17978/147‑1 (MQ=255) acTGCTGGGCGAGCATACCGAAGTGATGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCACCGCTTTGTTTTTCAGAGCTATCa > 7:188738/1‑147 (MQ=255) tgGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑aa < 3:240584/149‑1 (MQ=255) gattACCGATGTGTTGTTGGGGGATGTGTTGGGGTTGTTTGTTGGCTAGTTACTGCGTCTGCGTTATGGTGGGGTGCTATAGTTTAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGaaa < 8:188738/145‑1 (MQ=25) tACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTaa < 2:89865/149‑1 (MQ=255) aCCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTa > 7:158164/1‑147 (MQ=255) atgtggtgCTGGGGGAAGTGTGGGGCGGGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCATCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTAAAGGtt < 2:294611/147‑1 (MQ=255) gtgCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATGAAAGGT‑AATTGAAATTAAAGGTTGACGc > 7:25759/1‑149 (MQ=255) gggggAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTAAAGGTTGACGCCctc > 7:177302/1‑148 (MQ=255) gggAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTAAAGGTTGACGCCCtctc > 1:79016/1‑148 (MQ=255) ggAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGGGGCCCTGCGGCCTGCCCCCGTTGGTTTTGACCGGAATCAAAAGTAAATTTAAATTAAAGG‑TGACGCCCgctcc > 3:20608/1‑147 (MQ=255) gtgtTGGGGGTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCGTTGTTTTTCAGCGCTATAAAAGGT‑AATTTAAAATAAAGGTTTAAGCCCTATCCAATcc > 1:133942/1‑149 (MQ=255) ttGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGGCTCCCCGCTTTGTTTTTCAGGGCTATCAAAGGT‑AATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCt > 6:74062/1‑149 (MQ=255) ttGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTAAAGGTTGACGCCCTCTCGAATcccc < 8:161378/148‑1 (MQ=255) tGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTACCCCCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTAAAAGTTGACGCCCTCACGAATCCCCTTATAATACGCCCCa > 4:65165/1‑149 (MQ=255) gCGAGCTACACCGTCTTCGTGATGCTGGTGTGCTATAGATGAAAAGCGTTGAGTCCGTCAGGCCTCCACGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGAGGCCCACt < 6:262374/149‑1 (MQ=37) gCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACa > 1:220195/1‑149 (MQ=255) ggggTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTc > 1:221504/1‑149 (MQ=255) ggggTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTc > 8:303467/1‑149 (MQ=255) gggTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTc < 8:177302/148‑1 (MQ=255) gTGCTGTAGATGAAATGCGGGGAGTCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTATAGGTTGACTCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCtt < 3:65165/149‑1 (MQ=25) ctctAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTga < 7:303467/148‑1 (MQ=255) gCGGGGAGGCCTGACGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTg < 6:143451/134‑1 (MQ=255) gCGGGGAGGCCTGACGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTg > 5:143451/1‑134 (MQ=255) gCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGtt > 6:153696/1‑148 (MQ=255) cGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAg > 3:44613/1‑149 (MQ=255) | GACGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGT‑AATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAG > NC_002947/170696‑170985 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |