Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,239,115 C→T 8.9% intergenic (‑90/‑285) argF ← / → PP_1080 ornithine carbamoyltransferase/molybdopterin‑binding oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,239,1150CT 8.9% 124.0 / 2.1 45intergenic (‑90/‑285)argF/PP_1080ornithine carbamoyltransferase/molybdopterin‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (20/21);  new base T (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01

ACCCCGAGCAATTCGTCGGTGGTGAAGTCCAGCAGGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTT  >  NC_002947/1238967‑1239256
                                                                                                                                                     |                                                                                                                                             
aCCCCGAGCAATTCGTCGGTGGTGAAGTCCAGCAGGGAGAGAAAGTGCCTAGCGCTAATGAGGCACTACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGc                                                                                                                                               <  8:213636/149‑1 (MQ=255)
 ccccGAGCAATTCGTCGGTGGTGAAGTCCAGCAGGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTTCGGCGTAAGACGCACGGGGCg                                                                                                                                              >  3:222181/1‑149 (MQ=255)
    cGAGCAATTCGTCGGTGGTGAAGTCCAGCAGGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCgg                                                                                                                                             >  6:302222/1‑147 (MQ=255)
    cGAGCAATTCGTCGGTGGTGAAGTCCAGCAGGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCgg                                                                                                                                             <  5:302222/147‑1 (MQ=255)
     gAGCAATTCGTCGGTGGTGAAGTCCAGCAGGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGAc                                                                                                                                           >  3:189557/1‑148 (MQ=255)
       gCAATTCGTCGGTGGTGAAGTCCAGCAGGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGa                                                                                                                                         >  5:17040/1‑148 (MQ=255)
           ttCGTCGGTGGTTAAGTCCAGCAGGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAg                                                                                                                                    <  1:119880/149‑1 (MQ=255)
               tcgGTGGTGAAGTCCAGCAGGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGa                                                                                                                                 >  2:183007/1‑148 (MQ=255)
                     gtgAAGTCCAGCAGGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGgcg                                                                                                                           <  3:81141/148‑1 (MQ=255)
                        aattCCAGCAGGGTGATAAAGTGCCTAGCGCTCATGAGTGAATACCTTATCTGCAACGGTTTGCAGGTCGACGGTA‑TGGTTTTTTTTTACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCaa                                                                                                                        <  6:17040/145‑1 (MQ=255)
                             cagcagGGCGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTAATCTGCAACGGTATGCAGGTAGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACta                                                                                                                   <  8:62424/148‑1 (MQ=255)
                             cagcagGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCTCAATAGta                                                                                                                   >  6:212365/1‑148 (MQ=255)
                             cagcagGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACtat                                                                                                                  <  7:296058/149‑1 (MQ=255)
                               gcagGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATaa                                                                                                                <  1:183007/149‑1 (MQ=255)
                                                       ctatctgTGACTACCTTATCTGCAAGGGTTTGCAGGTCGTCCGTT‑TGGTTTTTTTTGACTACGGGGGTTAACTGCGGCGTAAGCGGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAggg                                                                                        <  4:23777/143‑1 (MQ=255)
                                                              tGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTCCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGgagatcg                                                                                 >  3:23777/1‑146 (MQ=255)
                                                                  tACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAgcgg                                                                             >  2:226555/1‑149 (MQ=255)
                                                                    ccTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAgcggc                                                                            <  5:289867/148‑1 (MQ=255)
                                                                    ccTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAgcggc                                                                            >  6:289867/1‑148 (MQ=255)
                                                                    ccTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAgcgg                                                                             <  1:226555/147‑1 (MQ=255)
                                                                    ccTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACATGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAATTAAATGTCGCCTGAAGCCAAGGGGGGAAAAgcggcg                                                                           >  1:278772/1‑149 (MQ=255)
                                                                     cttttgtGCAACGGTAGGCCGGTCCACCGTA‑TGTTTTTTTTTTACAACGGGGGTCACGTGCGGGGTACGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGt                                                                          <  2:278772/143‑1 (MQ=255)
                                                                      ttATCTGCAACGGTATGCAGGTCGACCGTAGTGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGt                                                                          >  1:192389/1‑149 (MQ=255)
                                                                         tCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAtt                                                                      >  2:282036/1‑149 (MQ=255)
                                                                             catgggTATTCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttg                                                                     <  4:189557/142‑1 (MQ=255)
                                                                             cAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGtctatgca                                                                   >  8:296543/1‑141 (MQ=255)
                                                                              aaCGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAAAACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgca                                                                   >  7:115747/1‑147 (MQ=255)
                                                                                           tCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagggtgttgcaaag                                                    <  6:186622/149‑1 (MQ=255)
                                                                                            ccaCCGTC‑TGGTTTTTTTTCACAACGGGGGGTACCTTCGGCGTAAGCGGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagggtgttgcaaagg                                                   <  8:115747/147‑1 (MQ=255)
                                                                                             gcccGTA‑TGTTTTTGTTTGACATCGGGGGTGCCCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGACAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGGGAttgcaaagggtgttgcaaaggg                                                  <  4:222181/147‑1 (MQ=255)
                                                                                             gACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAATTAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagggtgttgcaaaggg                                                  >  5:259836/1‑149 (MQ=255)
                                                                                                                gACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGcc                                >  7:5847/1‑149 (MQ=255)
                                                                                                                   aaCGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGt                             >  3:295230/1‑149 (MQ=255)
                                                                                                                       ggggTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTg                         <  2:192389/149‑1 (MQ=255)
                                                                                                                          gTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagggtgtt                                                          <  4:285560/113‑1 (MQ=255)
                                                                                                                          gTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagggtgtt                                                          >  3:285560/1‑113 (MQ=255)
                                                                                                                              cgtGCGGCTTACGCCGCACGGGGTGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAATGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTa                  >  2:187764/3‑149 (MQ=255)
                                                                                                                              cgtGCGGCTTACGCCGCACGGGGTGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAATGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTa                  >  2:192412/3‑149 (MQ=255)
                                                                                                                              ccggcggcgTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTATATGTCGCCTGAAGCCAAGGGGTGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTa                  <  5:212365/146‑1 (MQ=255)
                                                                                                                               cTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTAt                 <  1:282036/149‑1 (MQ=255)
                                                                                                                               cTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTAt                 >  6:201179/1‑149 (MQ=255)
                                                                                                                                           ccGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGc             <  8:123222/141‑1 (MQ=255)
                                                                                                                                           ccGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGc             >  7:123222/1‑141 (MQ=255)
                                                                                                                                              cACGGGGTGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAATGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCTCGTGCTGTTCTTTATTTGCTACAGACGGtt  <  1:187764/149‑1 (MQ=255)
                                                                                                                                              cACGGGGTGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAATGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGtt  <  1:192412/149‑1 (MQ=255)
                                                                                                                                              cACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGtt  <  8:5847/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                             
ACCCCGAGCAATTCGTCGGTGGTGAAGTCCAGCAGGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTT  >  NC_002947/1238967‑1239256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑