Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,239,168 G→T 7.6% intergenic (‑143/‑232) argF ← / → PP_1080 ornithine carbamoyltransferase/molybdopterin‑binding oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,239,1680GT 7.6% 153.6 / 3.6 53intergenic (‑143/‑232)argF/PP_1080ornithine carbamoyltransferase/molybdopterin‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (26/23);  new base T (2/2);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.89e-01

TCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTTGTACACGGCTTGCTGGCAATTTGGCAAATGGCTGTCGTAAATCGTGCGCCTGATGTCG  >  NC_002947/1239022‑1239314
                                                                                                                                                   |                                                                                                                                                  
ctatctgTGACTACCTTATCTGCAAGGGTTTGCAGGTCGTCCGTT‑TGGTTTTTTTTGACTACGGGGGTTAACTGCGGCGTAAGCGGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAggg                                                                                                                                                  <  4:23777/143‑1 (MQ=255)
       tGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTCCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGgagatcg                                                                                                                                           >  3:23777/1‑146 (MQ=255)
           tACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAgcgg                                                                                                                                       >  2:226555/1‑149 (MQ=255)
             ccTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAgcggc                                                                                                                                      <  5:289867/148‑1 (MQ=255)
             ccTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAgcggc                                                                                                                                      >  6:289867/1‑148 (MQ=255)
             ccTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAgcgg                                                                                                                                       <  1:226555/147‑1 (MQ=255)
             ccTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACATGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAATTAAATGTCGCCTGAAGCCAAGGGGGGAAAAgcggcg                                                                                                                                     >  1:278772/1‑149 (MQ=255)
              cttttgtGCAACGGTAGGCCGGTCCACCGTA‑TGTTTTTTTTTTACAACGGGGGTCACGTGCGGGGTACGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGt                                                                                                                                    <  2:278772/143‑1 (MQ=255)
               ttATCTGCAACGGTATGCAGGTCGACCGTAGTGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGt                                                                                                                                    >  1:192389/1‑149 (MQ=255)
                  tCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAtt                                                                                                                                >  2:282036/1‑149 (MQ=255)
                      catgggTATTCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttg                                                                                                                               <  4:189557/142‑1 (MQ=255)
                      cAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGtctatgca                                                                                                                             >  8:296543/1‑141 (MQ=255)
                       aaCGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAAAACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgca                                                                                                                             >  7:115747/1‑147 (MQ=255)
                                    tCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagggtgttgcaaag                                                                                                              <  6:186622/149‑1 (MQ=255)
                                     ccaCCGTC‑TGGTTTTTTTTCACAACGGGGGGTACCTTCGGCGTAAGCGGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagggtgttgcaaagg                                                                                                             <  8:115747/147‑1 (MQ=255)
                                      gcccGTA‑TGTTTTTGTTTGACATCGGGGGTGCCCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGACAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGGGAttgcaaagggtgttgcaaaggg                                                                                                            <  4:222181/147‑1 (MQ=255)
                                      gACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAATTAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagggtgttgcaaaggg                                                                                                            >  5:259836/1‑149 (MQ=255)
                                                         gACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGcc                                                                                          >  7:5847/1‑149 (MQ=255)
                                                            aaCGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGt                                                                                       >  3:295230/1‑149 (MQ=255)
                                                                ggggTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTg                                                                                   <  2:192389/149‑1 (MQ=255)
                                                                   gTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagggtgtt                                                                                                                    >  3:285560/1‑113 (MQ=255)
                                                                   gTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagggtgtt                                                                                                                    <  4:285560/113‑1 (MQ=255)
                                                                       cgtGCGGCTTACGCCGCACGGGGTGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAATGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTa                                                                            >  2:187764/3‑149 (MQ=255)
                                                                       cgtGCGGCTTACGCCGCACGGGGTGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAATGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTa                                                                            >  2:192412/3‑149 (MQ=255)
                                                                       ccggcggcgTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTATATGTCGCCTGAAGCCAAGGGGTGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTa                                                                            <  5:212365/146‑1 (MQ=255)
                                                                        cTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTAt                                                                           <  1:282036/149‑1 (MQ=255)
                                                                        cTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTAt                                                                           >  6:201179/1‑149 (MQ=255)
                                                                                    ccGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGc                                                                       <  8:123222/141‑1 (MQ=255)
                                                                                    ccGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGc                                                                       >  7:123222/1‑141 (MQ=255)
                                                                                       cACGGGGTGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAATGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCTCGTGCTGTTCTTTATTTGCTACAGACGGtt                                                            <  1:187764/149‑1 (MQ=255)
                                                                                       cACGGGGTGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAATGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGtt                                                            <  1:192412/149‑1 (MQ=255)
                                                                                       cACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGtt                                                            <  8:5847/149‑1 (MQ=255)
                                                                                               ggACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTTGTACACgg                                                    >  1:285116/1‑149 (MQ=255)
                                                                                                gACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTTGTACACGGc                                                   <  2:285116/149‑1 (MQ=255)
                                                                                                     attAGGGAAAGGCTCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTTGTACACGGCTTGCt                                              <  6:259836/147‑1 (MQ=255)
                                                                                                          ggAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTTGTACACGGCTTGCTGGCaa                                         >  6:94914/1‑149 (MQ=255)
                                                                                                           gAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGc                                                                       >  5:97917/1‑118 (MQ=255)
                                                                                                           gAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGc                                                                       <  6:97917/118‑1 (MQ=255)
                                                                                                             aaGGCGCAATACTATAAGTAAATGTCTCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTTGTACACGGCTTGCTGGCAAttt                                      <  7:296543/149‑1 (MQ=255)
                                                                                                             aaGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTTGTACACGGCTTGCTGGCAAttt                                      <  5:94914/149‑1 (MQ=255)
                                                                                                                gcgcAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTAttt                                                                         <  6:329072/111‑1 (MQ=255)
                                                                                                                gcgcAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTAttt                                                                         >  5:329072/1‑111 (MQ=255)
                                                                                                                    aaTACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTTGTACACGGCTTGCTGGCAATTTGGCAAAt                               <  5:201179/149‑1 (MQ=255)
                                                                                                                            aaGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTTGTACACGGCTTGCTGGCAATTTGGCAAATGGCTGTCg                       >  4:303699/1‑149 (MQ=255)
                                                                                                                                       gCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTTGTACACGGCTTGCTGGCAATTTGGCAAATGGCTGTCGTAAATCGTgag            >  5:169690/1‑147 (MQ=255)
                                                                                                                                        ccTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTTGTACACGGCTTGCTGGCAATTTGGCAAATGGCTGTCGTAAAt                  <  3:121822/142‑1 (MQ=255)
                                                                                                                                        ccTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTATTTATTTGCTACAGACGGTTGTACACGGCTTGCTGGCAATTTGGCAAATGGCTGTCGTAAAt                  >  4:121822/1‑142 (MQ=255)
                                                                                                                                         cTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTTGTACACGGCTTGCTGGCAATTTGGCAAATGGCTGTCGTAAATc                 <  7:172498/142‑1 (MQ=255)
                                                                                                                                         cTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTTGTAAACGGCTTGCTGGCAATTTGGCAAATGGCTGTCGTAAATc                 >  8:172498/1‑142 (MQ=255)
                                                                                                                                               ccAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTTGTACACGGCTTGCTGGCAATTTGGCAAATGGCTGTCGTAAATCGTgc             >  7:1043/1‑140 (MQ=255)
                                                                                                                                               ccAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTTGTACACGGCTTGCTGGCAATTTGGCAAATGGCTGTCGTAAATCGTgc             <  8:1043/140‑1 (MQ=255)
                                                                                                                                                 aaGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTTGTACACGGCTTGCTGGCAATTTGGCAAATGGCTGTCGTAAATCGTGCGCCTGATGTCg  >  3:230803/1‑149 (MQ=255)
                                                                                                                                                 aaGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTTGTACACGGCTTGCTGGCAATTTGGCAAATGGCTGTCGTAAATCGTGCGCCTGATGTCg  >  2:317503/1‑149 (MQ=255)
                                                                                                                                                 aaGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTTGTACACGGCTTGCTGGCAATTTGGCAAATGGCTGTCGTAAATCGTGCGCCTGATGTCg  >  5:298351/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTA‑TGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTACAGACGGTTGTACACGGCTTGCTGGCAATTTGGCAAATGGCTGTCGTAAATCGTGCGCCTGATGTCG  >  NC_002947/1239022‑1239314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑