Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,261,542	T→A	6.7%	S365C (AGC→TGC)	PP_1104 ←	AotO‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,261,542	0	T	A	6.7%	212.3 / 3.1	70	S365C (AGC→TGC)	PP_1104	AotO‑related protein
Reads supporting (aligned to +/- strand):  ref base T (34/31);  new base A (3/2);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTCCAGCCGCGCTTGGCAAACCAGGCATTGGCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTCGGCGATCA  >  NC_002947/1261396‑1261688                                                                                                                                                    |                                                                                                                                                   gCTTCCAGCCGCGCTTGGCAAACCAGGCATTGGCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGc                                                                                                                                                  <  5:44038/149‑1 (MQ=255)   ttCCAGCCGCGCTTGGCAAACCAGGCATTGGCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCgg                                                                                                                                                >  5:225390/1‑149 (MQ=255)        gCCGCGCTTGGCAAACCAGGCATTGGCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCTTAc                                                                                                                                           >  7:206298/1‑149 (MQ=255)        gCCGCGCTTGGCAAACCAGGCATTGGCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATAc                                                                                                                                           >  5:62479/1‑149 (MQ=255)          cgcgCTTGGCAAACCAGGCATTGGCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCt                                                                                                                                         >  2:1216/1‑149 (MQ=255)              cTTGGCAAACCAGGCATTGGCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTc                                                                                                                                     >  4:71891/1‑149 (MQ=255)                 ggCAAACCAGGCATTGGCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGcc                                                                                                                                  >  8:100249/1‑149 (MQ=255)                   cAAACCAGGCATTGGCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCgg                                                                                                                                >  1:32238/1‑149 (MQ=255)                    aaaCCAGGCATTGGCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGc                                                                                                                               >  3:311202/1‑149 (MQ=255)                    aaaCCAGGCATTGGCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGc                                                                                                                               <  5:182442/149‑1 (MQ=255)                            cATTGGCGAGGTCGGTAGCAGCAGGCAGGAGGGCAGGCGGTCCCTCGAAGGCTGGGCTTCTACAGACCACTTGCCGGCTGCAATGGCTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATggg                                                                                                                       <  6:300047/149‑1 (MQ=255)                              ttGGCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCg                                                                                                                     <  2:192051/149‑1 (MQ=255)                                  cGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGa                                                                                                                 <  6:62479/149‑1 (MQ=255)                                      gTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCAt                                                                                                             <  6:127924/149‑1 (MQ=255)                                        cGGTAGCAGCAGGCCTGATGGCAGGCGGTCCTTCGAAGGCTGGGCTTGTAGAGACCACTTGCCGGCCGCAATGGTTTTACCGGTTGAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGc                                                                                                           <  4:214281/149‑1 (MQ=255)                                              cagcagGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTg                                                                                                                                        >  7:34505/1‑114 (MQ=255)                                              cagcagGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTg                                                                                                                                        <  8:34505/114‑1 (MQ=255)                                                 cagGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGc                                                                                                                                               <  7:304511/104‑1 (MQ=255)                                                 cagGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGc                                                                                                                                               >  8:304511/1‑104 (MQ=255)                                                 cagGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTgg                                                                                                  <  3:94339/149‑1 (MQ=255)                                                 cagGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTgg                                                                                                  <  1:1216/149‑1 (MQ=255)                                                  agGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGc                                                                                                 <  1:294802/149‑1 (MQ=255)                                                   ggCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGc                                                                                                 >  3:141019/1‑148 (MQ=255)                                                    gCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGc                                                                                                                               >  4:64647/1‑117 (MQ=255)                                                    gCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGc                                                                                                                               <  3:64647/117‑1 (MQ=255)                                                    gCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCa                                                                                               <  3:71891/149‑1 (MQ=255)                                                          gggCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGcc                                                                                         >  5:283747/1‑149 (MQ=255)                                                           ggCAGGCGGGCCTTCGAAGGCTGGGCTTCTACAGACCACTTGCCGGCTTCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATCCCAGCGGTGGCCATGCGCCg                                                                                        <  8:126262/149‑1 (MQ=255)                                                            gCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCg                                                                                        >  2:117857/1‑148 (MQ=255)                                                             cAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTATGCCGCAGCGGC‑TACCTGttg                                                                                                                                     >  4:96541/1‑101 (MQ=38)                                                             cAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTATGCCGCAGCGGC‑TACCTGttg                                                                                                                                     <  3:96541/102‑2 (MQ=38)                                                              aGGCGTTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGAc                                                                                     <  6:225390/149‑1 (MQ=255)                                                                   gTCCTTCGAAGGCTGTGCTTCTACGGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTAc                                                                                >  7:183259/1‑149 (MQ=255)                                                                          gAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCAt                                                                          <  4:311202/148‑1 (MQ=255)                                                                          gAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATa                                                                         <  4:141019/149‑1 (MQ=255)                                                                           aaGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATa                                                                         >  5:121448/1‑148 (MQ=255)                                                                                       cTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCggc                                                            >  7:214804/1‑149 (MQ=255)                                                                                       cTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCggc                                                            >  7:19176/1‑149 (MQ=255)                                                                                       cTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCg                                                              <  6:121448/147‑1 (MQ=255)                                                                                           aGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCggctggc                                                        >  6:140090/1‑149 (MQ=255)                                                                                               cACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGtt                                                    >  4:270145/1‑149 (MQ=255)                                                                                                    gCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCACTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTgcg                                                 >  1:227971/1‑147 (MQ=255)                                                                                                     ccGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAg                                              >  5:243280/1‑149 (MQ=255)                                                                                                             aaTGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCGTGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGa                                      >  8:3497/1‑149 (MQ=255)                                                                                                              aTGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGAc                                     >  5:33700/1‑149 (MQ=255)                                                                                                                 gTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCg                                  <  7:100249/149‑1 (MQ=255)                                                                                                                    ttACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGt                                >  2:232571/1‑148 (MQ=255)                                                                                                                      aCCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCAc                             >  7:262616/1‑149 (MQ=255)                                                                                                                        cGGATCTATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACt                            >  5:42339/1‑148 (MQ=255)                                                                                                                        cGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACt                            >  8:40168/1‑148 (MQ=255)                                                                                                                          gATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGAc                                     <  3:18403/137‑1 (MQ=255)                                                                                                                          gATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGAc                                     >  4:18403/1‑137 (MQ=255)                                                                                                                           aTCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGt                        <  1:232571/149‑1 (MQ=255)                                                                                                                              aaTTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTggg                     <  6:243280/149‑1 (MQ=255)                                                                                                                               aTTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGt                        >  2:265827/1‑145 (MQ=255)                                                                                                                               aTTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGCCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGt                        <  1:265827/145‑1 (MQ=255)                                                                                                                                ttGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTc                   >  3:244939/1‑149 (MQ=255)                                                                                                                                 cggCGAACAGGTATGCCGCAGCGGC‑TACCTGTTGGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTc                   >  5:173007/2‑148 (MQ=38)                                                                                                                                   gCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGAt                <  8:19176/149‑1 (MQ=255)                                                                                                                                    ccAACCGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCCGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCCGCCCCGGCTGGCGGTGGCGCAGGGGGGTGCATCGGTCACTGCGTTGGGCGAt                >  1:197908/1‑148 (MQ=255)                                                                                                                                     cAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTAcgcg                                                                             >  4:182714/1‑86 (MQ=255)                                                                                                                                     cAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTAcgcg                                                                             <  3:182714/86‑1 (MQ=255)                                                                                                                                         aGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTc          <  2:32238/149‑1 (MQ=255)                                                                                                                                           gtatgCCGCAGCGGC‑TACCTGTTGGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTCgg        >  5:203640/5‑149 (MQ=255)                                                                                                                                           gTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTCgg        <  6:33700/149‑1 (MQ=255)                                                                                                                                                ccGCTGCGGCATACCTGTTCGCCGGCCACCTGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTCGGCGAt    <  6:183247/149‑1 (MQ=255)                                                                                                                                                ccGCAGCGGC‑TACCTGTTGGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTCGGCGAt    <  6:203640/148‑1 (MQ=255)                                                                                                                                                 cGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTCGGCGAt    >  5:190299/1‑148 (MQ=255)                                                                                                                                                 cGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTCGGCGATc   <  5:140090/149‑1 (MQ=255)                                                                                                                                                  gCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTCGGCGATCa  <  8:262616/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GCTTCCAGCCGCGCTTGGCAAACCAGGCATTGGCGAGGTCGGTAGCAGCAGGCATGAGGGCAGGCGGTCCTTCGAAGGCTGTGCTTCTACAGACCACTTGCCGGCTGCAATGGTTTTACCGGATCAATTGGCCAACAGGTA‑GCCGCTGCGGCATACCTGTTCGCCGGCCACATGGGCGATGACCATGCCAGCGGTGGCCATGCGCCGGACGCTACGCGCATACAGCAGCCCCGGCTGGCTGTTGCGCAGGGGGGTGACTCGGTCACTGAGTGGGTCGATGACTTCGGCGATCA  >  NC_002947/1261396‑1261688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑