Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,288,770	A→C	12.3%	S15R (AGC→CGC)	PP_1126 →	hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,288,770	0	A	C	12.3%	155.1 / 13.7	57	S15R (AGC→CGC)	PP_1126	hydrolase
Reads supporting (aligned to +/- strand):  ref base A (22/28);  new base C (3/4);  total (25/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

ATCCAGGCCTGCGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCGGGCAGCAAACG  >  NC_002947/1288625‑1288913                                                                                                                                                  |                                                                                                                                                aTCCAGGCCTGCGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGc                                                                                                                                               <  5:9566/148‑1 (MQ=255) aTCCAGGCCTGCGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCa                                                                                                                                              >  6:216328/1‑149 (MQ=255) aTCCAGGCCTGCGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCa                                                                                                                                              <  2:216835/149‑1 (MQ=255)    cAGGCCTGCGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACtt                                                                                                                                           <  4:115221/149‑1 (MQ=255)     aGGCCTGCGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTg                                                                                                                                          >  3:117492/1‑149 (MQ=255)      ggCCTGCGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTgc                                                                                                                                         <  2:162007/149‑1 (MQ=255)           gCGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCgg                                                                                                                                    <  7:242254/149‑1 (MQ=255)            cGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCg                                                                                                                                     <  1:149684/147‑1 (MQ=255)            cGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCg                                                                                                                                     <  2:150324/147‑1 (MQ=255)               ccgccTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGCGCAAGTGCTCCGgcgat                                                                                                                                <  4:198938/149‑3 (MQ=255)               ccgccTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGCGCAAGTGCTCCGgcgat                                                                                                                                <  1:293829/149‑3 (MQ=255)                 gccTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCAtt                                                                                                                              <  5:196685/149‑1 (MQ=255)                         cGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCAtt                                                                                                                       <  2:80320/148‑1 (MQ=255)                         cGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCAt                                                                                                                        <  5:114136/147‑1 (MQ=255)                               gAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTcc                                                                                                                          <  2:243103/139‑1 (MQ=255)                               gAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTcc                                                                                                                          >  1:243103/1‑139 (MQ=255)                                aGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGCGCAAGTGCTCCGgcgatca                                                                                                                              <  6:257387/134‑5 (MQ=255)                                aGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGCGCAAGTGCTCCGgcgatca                                                                                                                              >  5:257387/1‑130 (MQ=255)                                         gcggcgTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCa                                                                                                       >  8:11708/1‑148 (MQ=255)                                             cgTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAgg                                                                                                  <  7:11708/149‑1 (MQ=255)                                             cgTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAgg                                                                                                  <  5:193566/149‑1 (MQ=255)                                                      cttgcttgACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAg                                                                                         >  4:128608/1‑149 (MQ=255)                                                      cttgcttgACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAg                                                                                         <  7:219616/149‑1 (MQ=255)                                                         gcttgACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAg                                                                                         >  6:66645/1‑146 (MQ=255)                                                          cttgACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGgaga                                                                                     <  2:315631/149‑1 (MQ=255)                                                           ttgACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGagag                                                                                    >  6:6446/1‑149 (MQ=255)                                                                  gcgcTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGc                                                                             >  5:214233/1‑149 (MQ=255)                                                                     cTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCtt                                                                          <  5:190212/149‑1 (MQ=255)                                                                        ggTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTa                                                                         <  2:99848/147‑1 (MQ=255)                                                                         gTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCAt                                                                                                                        <  2:75563/99‑1 (MQ=255)                                                                         gTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCAt                                                                                                                        >  1:75563/1‑99 (MQ=255)                                                                         gTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGa                                                                      <  4:268973/149‑1 (MQ=255)                                                                            aCGCAGCGCTACGGCAAGGCTATTCTCACTGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGCTCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTc                                                                                                                   <  7:293017/101‑1 (MQ=255)                                                                            aCGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCCCTTGCGCCGGCGCCTTGTCCAGTAcga                                                                                                                   >  8:293017/1‑99 (MQ=255)                                                                                       cGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCt                                                         <  8:118574/148‑1 (MQ=255)                                                                                           aaGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCccg                                                    >  4:67679/1‑149 (MQ=255)                                                                                                 aTTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTAt                                              >  6:138821/1‑149 (MQ=255)                                                                                                    ctcAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTc                                            <  5:142342/148‑1 (MQ=255)                                                                                                     tcAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGc                                          >  1:13771/1‑149 (MQ=255)                                                                                                      cAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGcc                                         >  3:7258/1‑149 (MQ=255)                                                                                                       aaTGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTgg                                                                                                            <  5:270324/81‑1 (MQ=255)                                                                                                       aaTGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTgg                                                                                                            >  6:270324/1‑81 (MQ=255)                                                                                                       aaTGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCt                                        >  8:181798/1‑149 (MQ=255)                                                                                                         tGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCAGCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCttg                                      <  5:207959/149‑1 (MQ=255)                                                                                                               gccgccGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTtgtg                                 >  7:129789/1‑148 (MQ=255)                                                                                                                ccgccgTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTTGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCa                               >  3:161159/1‑149 (MQ=255)                                                                                                                ccgccgTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTtgtg                                 <  5:66645/147‑1 (MQ=255)                                                                                                                      ttCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGGATTCGCCTTGTTGTGCAGTTCGc                         >  7:290018/1‑149 (MQ=255)                                                                                                                        ccGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCtt                                                                          <  4:310302/98‑1 (MQ=255)                                                                                                                        ccGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCtt                                                                          >  3:310302/1‑98 (MQ=255)                                                                                                                               gAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTtgtg                                 >  2:53575/1‑132 (MQ=255)                                                                                                                                aaaTTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGc               >  3:86961/1‑149 (MQ=255)                                                                                                                                     tCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCggg           <  5:216328/148‑1 (MQ=255)                                                                                                                                        aatgcGCCGGCGCAAGTGCTCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCGGgcag        >  2:286191/5‑148 (MQ=255)                                                                                                                                         atgcGCCGGCGCAAGTGCTCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGcc                                         >  2:73107/4‑114 (MQ=255)                                                                                                                                         atgcGCCGGCGCAAGTGCTCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGcc                                         <  1:73107/111‑1 (MQ=255)                                                                                                                                             gCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCGGGCAGCAAACg  <  4:7258/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                ATCCAGGCCTGCGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCGGGCAGCAAACG  >  NC_002947/1288625‑1288913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑