Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,288,774 C→A 12.7% T16N (ACT→AAT) ‡ PP_1126 → hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,288,7740CA12.7% 157.6 / 12.7 55T16N (ACT→AAT) ‡PP_1126hydrolase
Reads supporting (aligned to +/- strand):  ref base C (21/27);  new base A (3/4);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

CAGGCCTGCGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCGGGCAGCAAACGCTGTTCAG  >  NC_002947/1288628‑1288921
                                                                                                                                                  |                                                                                                                                                   
cAGGCCTGCGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACtt                                                                                                                                                   <  4:115221/149‑1 (MQ=255)
 aGGCCTGCGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTg                                                                                                                                                  >  3:117492/1‑149 (MQ=255)
  ggCCTGCGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTgc                                                                                                                                                 <  2:162007/149‑1 (MQ=255)
       gCGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCgg                                                                                                                                            <  7:242254/149‑1 (MQ=255)
        cGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCg                                                                                                                                             <  1:149684/147‑1 (MQ=255)
        cGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCg                                                                                                                                             <  2:150324/147‑1 (MQ=255)
           ccgccTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGCGCAAGTGCTCCGgcgat                                                                                                                                        <  4:198938/149‑3 (MQ=255)
           ccgccTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGCGCAAGTGCTCCGgcgat                                                                                                                                        <  1:293829/149‑3 (MQ=255)
             gccTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCAtt                                                                                                                                      <  5:196685/149‑1 (MQ=255)
                     cGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCAtt                                                                                                                               <  2:80320/148‑1 (MQ=255)
                     cGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCAt                                                                                                                                <  5:114136/147‑1 (MQ=255)
                           gAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTcc                                                                                                                                  <  2:243103/139‑1 (MQ=255)
                           gAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTcc                                                                                                                                  >  1:243103/1‑139 (MQ=255)
                            aGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGCGCAAGTGCTCCGgcgatca                                                                                                                                      <  6:257387/134‑5 (MQ=255)
                            aGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGCGCAAGTGCTCCGgcgatca                                                                                                                                      >  5:257387/1‑130 (MQ=255)
                                     gcggcgTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCa                                                                                                               >  8:11708/1‑148 (MQ=255)
                                         cgTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAgg                                                                                                          <  7:11708/149‑1 (MQ=255)
                                         cgTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAgg                                                                                                          <  5:193566/149‑1 (MQ=255)
                                                  cttgcttgACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAg                                                                                                 <  7:219616/149‑1 (MQ=255)
                                                  cttgcttgACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAg                                                                                                 >  4:128608/1‑149 (MQ=255)
                                                     gcttgACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAg                                                                                                 >  6:66645/1‑146 (MQ=255)
                                                      cttgACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGgaga                                                                                             <  2:315631/149‑1 (MQ=255)
                                                       ttgACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGagag                                                                                            >  6:6446/1‑149 (MQ=255)
                                                              gcgcTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGc                                                                                     >  5:214233/1‑149 (MQ=255)
                                                                 cTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCtt                                                                                  <  5:190212/149‑1 (MQ=255)
                                                                    ggTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTa                                                                                 <  2:99848/147‑1 (MQ=255)
                                                                     gTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCAt                                                                                                                                <  2:75563/99‑1 (MQ=255)
                                                                     gTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCAt                                                                                                                                >  1:75563/1‑99 (MQ=255)
                                                                     gTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGa                                                                              <  4:268973/149‑1 (MQ=255)
                                                                        aCGCAGCGCTACGGCAAGGCTATTCTCACTGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGCTCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTc                                                                                                                           <  7:293017/101‑1 (MQ=255)
                                                                        aCGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCCCTTGCGCCGGCGCCTTGTCCAGTAcga                                                                                                                           >  8:293017/1‑99 (MQ=255)
                                                                                   cGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCt                                                                 <  8:118574/148‑1 (MQ=255)
                                                                                       aaGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCccg                                                            >  4:67679/1‑149 (MQ=255)
                                                                                             aTTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTAt                                                      >  6:138821/1‑149 (MQ=255)
                                                                                                ctcAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTc                                                    <  5:142342/148‑1 (MQ=255)
                                                                                                 tcAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGc                                                  >  1:13771/1‑149 (MQ=255)
                                                                                                  cAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGcc                                                 >  3:7258/1‑149 (MQ=255)
                                                                                                   aaTGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTgg                                                                                                                    <  5:270324/81‑1 (MQ=255)
                                                                                                   aaTGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTgg                                                                                                                    >  6:270324/1‑81 (MQ=255)
                                                                                                   aaTGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCt                                                >  8:181798/1‑149 (MQ=255)
                                                                                                     tGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCAGCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCttg                                              <  5:207959/149‑1 (MQ=255)
                                                                                                           gccgccGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTtgtg                                         >  7:129789/1‑148 (MQ=255)
                                                                                                            ccgccgTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTTGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCa                                       >  3:161159/1‑149 (MQ=255)
                                                                                                            ccgccgTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTtgtg                                         <  5:66645/147‑1 (MQ=255)
                                                                                                                  ttCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGGATTCGCCTTGTTGTGCAGTTCGc                                 >  7:290018/1‑149 (MQ=255)
                                                                                                                    ccGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCtt                                                                                  >  3:310302/1‑98 (MQ=255)
                                                                                                                    ccGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCtt                                                                                  <  4:310302/98‑1 (MQ=255)
                                                                                                                           gAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTtgtg                                         >  2:53575/1‑132 (MQ=255)
                                                                                                                            aaaTTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGc                       >  3:86961/1‑149 (MQ=255)
                                                                                                                                 tCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCggg                   <  5:216328/148‑1 (MQ=255)
                                                                                                                                    aatgcGCCGGCGCAAGTGCTCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCGGgcag                >  2:286191/5‑148 (MQ=255)
                                                                                                                                     atgcGCCGGCGCAAGTGCTCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGcc                                                 >  2:73107/4‑114 (MQ=255)
                                                                                                                                     atgcGCCGGCGCAAGTGCTCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGcc                                                 <  1:73107/111‑1 (MQ=255)
                                                                                                                                         gCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCGGGCAGCAAACg          <  4:7258/149‑1 (MQ=255)
                                                                                                                                                 aCTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCGGGCAGCAAACGCTGTTCAg  <  3:128608/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CAGGCCTGCGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCGGGCAGCAAACGCTGTTCAG  >  NC_002947/1288628‑1288921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑