Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,288,779 G→T 13.2% A18S (GCC→TCC)  PP_1126 → hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,288,7790GT13.2% 151.2 / 13.0 53A18S (GCC→TCC) PP_1126hydrolase
Reads supporting (aligned to +/- strand):  ref base G (21/25);  new base T (3/4);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.37e-01

GCGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCGGGCAGCAAACGCTGTTCAGCTTCGT  >  NC_002947/1288635‑1288927
                                                                                                                                                |                                                                                                                                                    
gCGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCgg                                                                                                                                                  <  7:242254/149‑1 (MQ=255)
 cGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCg                                                                                                                                                   <  1:149684/147‑1 (MQ=255)
 cGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCg                                                                                                                                                   <  2:150324/147‑1 (MQ=255)
    ccgccTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGCGCAAGTGCTCCGgcgat                                                                                                                                              <  1:293829/149‑3 (MQ=255)
    ccgccTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGCGCAAGTGCTCCGgcgat                                                                                                                                              <  4:198938/149‑3 (MQ=255)
      gccTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCAtt                                                                                                                                            <  5:196685/149‑1 (MQ=255)
              cGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCAtt                                                                                                                                     <  2:80320/148‑1 (MQ=255)
              cGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCAt                                                                                                                                      <  5:114136/147‑1 (MQ=255)
                    gAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTcc                                                                                                                                        >  1:243103/1‑139 (MQ=255)
                    gAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTcc                                                                                                                                        <  2:243103/139‑1 (MQ=255)
                     aGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGCGCAAGTGCTCCGgcgatca                                                                                                                                            >  5:257387/1‑130 (MQ=255)
                     aGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGCGCAAGTGCTCCGgcgatca                                                                                                                                            <  6:257387/134‑5 (MQ=255)
                              gcggcgTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCa                                                                                                                     >  8:11708/1‑148 (MQ=255)
                                  cgTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAgg                                                                                                                <  5:193566/149‑1 (MQ=255)
                                  cgTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAgg                                                                                                                <  7:11708/149‑1 (MQ=255)
                                           cttgcttgACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAg                                                                                                       >  4:128608/1‑149 (MQ=255)
                                           cttgcttgACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAg                                                                                                       <  7:219616/149‑1 (MQ=255)
                                              gcttgACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAg                                                                                                       >  6:66645/1‑146 (MQ=255)
                                               cttgACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGgaga                                                                                                   <  2:315631/149‑1 (MQ=255)
                                                ttgACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGagag                                                                                                  >  6:6446/1‑149 (MQ=255)
                                                       gcgcTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGc                                                                                           >  5:214233/1‑149 (MQ=255)
                                                          cTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCtt                                                                                        <  5:190212/149‑1 (MQ=255)
                                                             ggTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTa                                                                                       <  2:99848/147‑1 (MQ=255)
                                                              gTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCAt                                                                                                                                      >  1:75563/1‑99 (MQ=255)
                                                              gTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCAt                                                                                                                                      <  2:75563/99‑1 (MQ=255)
                                                              gTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGa                                                                                    <  4:268973/149‑1 (MQ=255)
                                                                 aCGCAGCGCTACGGCAAGGCTATTCTCACTGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGCTCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTc                                                                                                                                 <  7:293017/101‑1 (MQ=255)
                                                                 aCGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCCCTTGCGCCGGCGCCTTGTCCAGTAcga                                                                                                                                 >  8:293017/1‑99 (MQ=255)
                                                                            cGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCt                                                                       <  8:118574/148‑1 (MQ=255)
                                                                                aaGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCccg                                                                  >  4:67679/1‑149 (MQ=255)
                                                                                      aTTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTAt                                                            >  6:138821/1‑149 (MQ=255)
                                                                                         ctcAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTc                                                          <  5:142342/148‑1 (MQ=255)
                                                                                          tcAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGc                                                        >  1:13771/1‑149 (MQ=255)
                                                                                           cAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGcc                                                       >  3:7258/1‑149 (MQ=255)
                                                                                            aaTGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTgg                                                                                                                          <  5:270324/81‑1 (MQ=255)
                                                                                            aaTGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTgg                                                                                                                          >  6:270324/1‑81 (MQ=255)
                                                                                            aaTGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCt                                                      >  8:181798/1‑149 (MQ=255)
                                                                                              tGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCAGCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCttg                                                    <  5:207959/149‑1 (MQ=255)
                                                                                                    gccgccGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTtgtg                                               >  7:129789/1‑148 (MQ=255)
                                                                                                     ccgccgTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTTGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCa                                             >  3:161159/1‑149 (MQ=255)
                                                                                                     ccgccgTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTtgtg                                               <  5:66645/147‑1 (MQ=255)
                                                                                                           ttCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGGATTCGCCTTGTTGTGCAGTTCGc                                       >  7:290018/1‑149 (MQ=255)
                                                                                                             ccGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCtt                                                                                        <  4:310302/98‑1 (MQ=255)
                                                                                                             ccGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCtt                                                                                        >  3:310302/1‑98 (MQ=255)
                                                                                                                    gAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTtgtg                                               >  2:53575/1‑132 (MQ=255)
                                                                                                                     aaaTTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGc                             >  3:86961/1‑149 (MQ=255)
                                                                                                                          tCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCggg                         <  5:216328/148‑1 (MQ=255)
                                                                                                                             aatgcGCCGGCGCAAGTGCTCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCGGgcag                      >  2:286191/5‑148 (MQ=255)
                                                                                                                              atgcGCCGGCGCAAGTGCTCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGcc                                                       <  1:73107/111‑1 (MQ=255)
                                                                                                                              atgcGCCGGCGCAAGTGCTCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGcc                                                       >  2:73107/4‑114 (MQ=255)
                                                                                                                                  gCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCGGGCAGCAAACg                <  4:7258/149‑1 (MQ=255)
                                                                                                                                          aCTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCGGGCAGCAAACGCTGTTCAg        <  3:128608/149‑1 (MQ=255)
                                                                                                                                            ttGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCGGGCAGCAAACGCTGTTCAGCt      >  8:304861/1‑149 (MQ=255)
                                                                                                                                                gcCGGCGCATTGTCCATTACTTGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCGGGCAGCAAACGCTGTTCAGCTTCGt  <  4:161159/149‑1 (MQ=255)
                                                                                                                                                gcCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCGGGCAGCAAACGCTGTTCAGCTTCGt  <  5:6446/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GCGGCCGCCTTCTGCGTACCGAGCAGGATCGCGGCGTTATCACCTTGCTTGACCGGCGCTTGGTCACGCAGCGCTACGGCAAGGCTATTCTCAATGCGCTGCCGCCGTTCCGGCGGGAAATTTCCTGATCGCCGGAGCACTTGCGCCGGCGCATTGTCCATTACTCGTCTTGGGCCCATCAGGGTCCTGAAGGAGAGCCTCAGCCTTATGATCCGCCGCACCCTGCCCGCCGTATTCGCCTTGTTGTGCAGTTCGCCCTTGCTGGCCGGGCAGCAAACGCTGTTCAGCTTCGT  >  NC_002947/1288635‑1288927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑