Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,330,563 T→G 12.5% intergenic (+94/‑213) PP_5SE → / → PP_1157 5S ribosomal RNA/acetolactate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,330,5630TG12.5% 103.3 / 7.0 40intergenic (+94/‑213)PP_5SE/PP_11575S ribosomal RNA/acetolactate synthase
Reads supporting (aligned to +/- strand):  ref base T (18/17);  new base G (3/2);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

ATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTA‑GGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGACCTGG  >  NC_002947/1330415‑1330708
                                                                                                                                                     |                                                                                                                                                 
aTCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTA‑GGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCt                                                                                                                                                   >  7:238785/1‑149 (MQ=255)
    ccGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTA‑GGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGc                                                                                                                                               <  7:43568/149‑1 (MQ=255)
         ggTAGTGTGGGGTTTCCCCATGTGAGAGTATGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCGAAGCCGgt                                                                                                                                           >  1:192486/1‑149 (MQ=17)
           tAGTGTGGGGTTTCCCCATGTGAGAGTA‑GGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCGAAGCCGgtgcc                                                                                                                                        <  2:192486/149‑3 (MQ=255)
                                      a‑GGTCTTCGTGAAGATTGTTTTCGCAAAACCCCTATGTTCGTACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTTGACCTGCTCGAGAGCGGAGATGGCTCCGGGTTCGGCGGTGTTCGCGGGTAAACCCGCTCCTACATAgg                                                                                                              <  8:256171/148‑1 (MQ=21)
                                        ggTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCGAAGCCGgtgcc                                                                                                                                        >  4:30424/1‑119 (MQ=255)
                                        ggTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCGAAGCCGgtgcc                                                                                                                                        <  3:30424/121‑3 (MQ=255)
                                         gTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATg                                                                                                           <  7:158600/149‑1 (MQ=255)
                                               gTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGTTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGACGGTGCg                                                                                                      <  1:234099/148‑1 (MQ=255)
                                               gTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCg                                                                                                      >  8:206310/1‑148 (MQ=255)
                                                             cGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAAt                                                                                       >  7:34456/1‑149 (MQ=255)
                                                                aaaaCCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGc                                                                                    <  7:206310/149‑1 (MQ=255)
                                                                      ccTATCTGCGCACGCAGGTAGGGATTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATCGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAAc                                                                              >  7:201129/1‑149 (MQ=255)
                                                                             gcgcACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCAt                                                                       >  3:134911/1‑149 (MQ=255)
                                                                               gcacgcaGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCAtt                                                                      >  7:166401/1‑148 (MQ=255)
                                                                                 acgcaGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAAc                                                                   <  4:268232/149‑1 (MQ=255)
                                                                                     aGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGa                                                               <  8:34456/149‑1 (MQ=255)
                                                                                     aGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGGTcgc                                                                                                                                     >  4:38936/1‑79 (MQ=255)
                                                                                     aGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGGTcgc                                                                                                                                     <  3:38936/79‑1 (MQ=255)
                                                                                              ttttGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAAc                                                                              >  1:283484/1‑125 (MQ=255)
                                                                                              ttttGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAAc                                                                              <  2:283484/125‑1 (MQ=255)
                                                                                                 tGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCAc                                                    <  4:134911/148‑1 (MQ=255)
                                                                                                               aaaaaTTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTc                                        >  8:95648/1‑146 (MQ=255)
                                                                                                                   aTTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACtt                                 >  5:88945/1‑149 (MQ=255)
                                                                                                                    ttGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACttt                                <  6:88945/149‑1 (MQ=255)
                                                                                                                          cTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCg                          >  6:292387/1‑149 (MQ=255)
                                                                                                                          cTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCg                          >  3:138269/1‑149 (MQ=255)
                                                                                                                          cTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCg                          >  1:144874/1‑149 (MQ=255)
                                                                                                                                gagaGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCAc                    <  8:238785/149‑1 (MQ=255)
                                                                                                                                gagaGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCAc                    >  2:120305/1‑149 (MQ=255)
                                                                                                                                       gagaTGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCt             >  1:219386/1‑149 (MQ=255)
                                                                                                                                         gcgaacACCGGCGAAGCCGGTGCTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTcc           >  8:41790/6‑149 (MQ=255)
                                                                                                                                           tGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCt          <  7:95648/148‑1 (MQ=255)
                                                                                                                                            ggCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAg                                                                                                   <  4:292067/58‑1 (MQ=255)
                                                                                                                                            ggCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAg                                                                                                   >  3:292067/1‑58 (MQ=255)
                                                                                                                                             gCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTg         <  5:2396/147‑1 (MQ=255)
                                                                                                                                             gCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGa       >  4:112973/1‑149 (MQ=255)
                                                                                                                                             gCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAAAGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCACCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTg         >  6:2396/1‑147 (MQ=255)
                                                                                                                                                ccGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGACCt    >  4:252622/1‑149 (MQ=255)
                                                                                                                                                  ggCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGACCTgg  <  2:219386/149‑1 (MQ=255)
                                                                                                                                                  ggCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGACCTgg  <  5:292387/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
ATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTA‑GGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGACCTGG  >  NC_002947/1330415‑1330708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑