Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,380,216 A→G 7.1% V20A (GTG→GCG)  PP_1202 ← phospholipids modification protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,380,2160AG 7.1% 162.9 / 2.7 56V20A (GTG→GCG) PP_1202phospholipids modification protein
Reads supporting (aligned to +/- strand):  ref base A (29/23);  new base G (2/2);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01

GGCATAGATGTCCAGCTCGCTCAGCAGGTGGCGGCAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGAAAGGTGCGGCCAAGTGAAAGTCCCTTAGGAAAACGTATCCAATGTTACTCCGGCC  >  NC_002947/1380086‑1380363
                                                                                                                                  |                                                                                                                                                   
ggCATAGATGTCCAGCTCGCTCAGCAGGTGGCGGCAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCg                                                                                                                                   >  5:245440/1‑149 (MQ=255)
  cATAGATGTCCAGCTCGCTCAGCAGGTGGCGGCAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCg                                                                                                                                   >  7:294043/1‑147 (MQ=255)
  cATAGATGTCCAGCTCGCTCAGCAGGTGGCGGCAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCg                                                                                                                                   >  2:64259/1‑147 (MQ=255)
   aTAGATGTCCAGCTCGCTCAGCAGGTGGCGGCAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGcc                                                                                                                                    >  1:214278/1‑145 (MQ=255)
   aTAGATGTCCAGCTCGCTCAGCAGGTGGCGGCAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGcc                                                                                                                                    <  2:214278/145‑1 (MQ=255)
          tCCAGCTCGCTCAGCAGGTGGCGGCAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTg                                                                                                                          <  8:294043/148‑1 (MQ=255)
           ccAGCTCGCTCAGCAGGTGGCGGCAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCGCAGCACCGGGTAGTGCCGTAGCGAGTGGc                                                                                                                        >  1:258713/1‑149 (MQ=255)
               ctcgctcAGCAGGTGGCGGCAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCg                                                                                                                    >  4:74063/1‑149 (MQ=255)
                 cgctcAGCAGGTGGCGGCAGGCAATCAAGGCCATCGAGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCg                                                                                                                    >  4:68100/1‑147 (MQ=255)
                  gctcAGCAGGTGGCGGCAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGa                                                                                                                  <  8:194753/148‑1 (MQ=255)
                  gctcAGCAGGTGGCGGCAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAg                                                                                                                 >  5:32122/1‑149 (MQ=255)
                    tcAGCAGGTGGCGGCAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCAc                                                                                                                      >  7:225072/1‑142 (MQ=255)
                    tcAGCAGGTGGCGGCAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCAc                                                                                                                      <  8:225072/142‑1 (MQ=255)
                    tcAGCAGGTGGCGGCAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGt                                                                                                               >  6:150766/1‑149 (MQ=255)
                      agcagGTGGCGGCAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTc                                                                                                             <  7:121983/149‑1 (MQ=255)
                        cagGTGGCGGCAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTa                                                                                                                                 >  3:99636/1‑127 (MQ=255)
                        cagGTGGCGGCAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTa                                                                                                                                 <  4:99636/127‑1 (MQ=255)
                          ggTGGCGGCAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGa                                                                                                         <  6:245440/149‑1 (MQ=255)
                               cggcAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATtgtg                                                                                                    <  1:18983/149‑1 (MQ=255)
                                ggcaggcaATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGc                                                                                                   <  1:174273/149‑1 (MQ=255)
                                   aggcaATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGTGCTGTGCAGCGcctgcc                                                                                                                                          >  6:309733/1‑103 (MQ=255)
                                   aggcaATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGTGCTGTGCAGCGcctgcc                                                                                                                                          <  5:309733/107‑5 (MQ=255)
                                     gcaATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGACGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGt                                                                                              >  8:145635/1‑149 (MQ=255)
                                            aGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGt                                                                                       >  4:136903/1‑149 (MQ=255)
                                            aGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGt                                                                                       >  4:250315/1‑149 (MQ=255)
                                                   cGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCg                                                                                >  4:17497/1‑149 (MQ=255)
                                                         cAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATg                                                                          >  7:56699/1‑149 (MQ=255)
                                                            tAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACCCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGc                                                                                                                               <  6:165333/93‑1 (MQ=255)
                                                            tAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACCCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGc                                                                                                                               >  5:165333/1‑93 (MQ=255)
                                                                 gggTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGa                                                                  <  6:32122/149‑1 (MQ=255)
                                                                  ggTCACCCTCAGGCCTATGGGCTGGCGGTCGCGACGCAGGCGTTCGAGGAGGGGCAGGCGCGGCGCAGCACCGGGGAGTGCCGTAGCGAGTGGCACCGGCGGTTCGGGATGGTGCGCAGTCATGAGTTGCCCCGGGCATGCAGCGCGAg                                                                 <  2:258713/149‑1 (MQ=255)
                                                                      aCCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCgg                                                                                                           >  2:119586/1‑103 (MQ=255)
                                                                      aCCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCgg                                                                                                           <  1:119586/103‑1 (MQ=255)
                                                                          tCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCgg                                                                                                                   >  5:229119/1‑91 (MQ=255)
                                                                          tCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCgg                                                                                                                   <  6:229119/91‑1 (MQ=255)
                                                                            aGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGaaa                                                       <  5:150766/149‑1 (MQ=255)
                                                                                   tGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGAAAGGTGc                                                  <  8:56699/147‑1 (MQ=255)
                                                                                      gCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACAGGAGGTTCGGGATTGTTCGAAGTCATGAGTTGCCCCGGGCATGCAACGCGAGGTCAAGGAAAGGTGCGGcc                                              >  1:238508/1‑148 (MQ=255)
                                                                                          gCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGAAAGGTGCGGCCAAGTg                                         <  3:250315/149‑1 (MQ=255)
                                                                                                 cGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGAAAGGTGCGGCCAAGTGAAAGTcc                                  <  3:74063/149‑1 (MQ=255)
                                                                                                   aCTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGAAAGGTGc                                                  <  7:8102/131‑1 (MQ=255)
                                                                                                   aCTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGAAAGGTGc                                                  >  8:8102/1‑131 (MQ=255)
                                                                                                     tCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGTGGTTCGGGATTGTGc                                                                                                   <  2:316515/80‑1 (MQ=255)
                                                                                                     tCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGTGGTTCGGGATTGTGc                                                                                                   >  1:316515/1‑80 (MQ=255)
                                                                                                        ggCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGAAAGGTGCGGCCAAGTGAAAGTCCCTTAgg                            >  8:189463/1‑148 (MQ=255)
                                                                                                         gCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGAAAGGTGCGGCCAAGTGAAAGTCCCTTAGGa                           >  1:185046/1‑148 (MQ=255)
                                                                                                            ttCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGAAAGGTGCGGCCAAGTGAAAGTCCCTTAGGAAAAc                       <  3:17497/149‑1 (MQ=255)
                                                                                                            ttCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGAAAGGTGCGGCCAAGTGAAAGTCCCTTAGGAAAAc                       <  1:64259/149‑1 (MQ=255)
                                                                                                               gAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGAAAGGTGCGGCCAAGTGAAAGTCCCTTAGGAAAACGTa                    >  1:297472/1‑149 (MQ=255)
                                                                                                                    ggggCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGAAAGGTGCGGCCAAGTGAAAGTCCCTTAGGAAAACGTATCCaa               >  4:194093/1‑149 (MQ=255)
                                                                                                                       gCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGAAAGGTGCGGCCAAGTGAAAGTCCCTTAGGAAAACGTATc                  >  4:216076/1‑143 (MQ=255)
                                                                                                                       gCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGAAAGGTGCGGCCAAGTGAAAGTCCCTTAGGAAAACGTATc                  <  3:216076/143‑1 (MQ=255)
                                                                                                                       gCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGAAAGGTGCGGCCAAGTGAAAGTCCCTTAGGAAAACGTATCCAATGt            >  6:318481/1‑149 (MQ=255)
                                                                                                                       gCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGAAAGGTGCGGCCAAGTGAAAGTCCCTTAGGAAAACGTATCCAATGt            <  7:189463/149‑1 (MQ=255)
                                                                                                                       gCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGAGCGAGGTGAAGGAAAGCTGCCGCCAAGTGAAAGTCACTTAGGCAAACGTATCCAATGt            >  8:298115/1‑149 (MQ=255)
                                                                                                                             gcTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGAAAGGTGCGGCCAAGTGAAAGTCCCTTAGGAAAACGTATCCAATGTTACTcc      >  1:155455/1‑149 (MQ=255)
                                                                                                                                 cacaGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGAAAGGTGCGGCCAAGTGAAAGTCCCTTAGGAAAACGTATCCAATGTTACTCCGGcc  >  5:240838/1‑149 (MQ=255)
                                                                                                                                  |                                                                                                                                                   
GGCATAGATGTCCAGCTCGCTCAGCAGGTGGCGGCAGGCAATCAAGGCCATCGTGAACAGTAACAGGGTCACCATCAGGCCTATGGGCTGGCGGTAGCGACTCAGGCGTTCGAGTAGGGGCAGGCGCTGCACAGCACCGGGTAGTGCCGTAGCGAGTGGCACCGGAGGTTCGGGATTGTGCGAAGTCATGAGTTGCCCCGGGCATGCAGCGCGAGGTGAAGGAAAGGTGCGGCCAAGTGAAAGTCCCTTAGGAAAACGTATCCAATGTTACTCCGGCC  >  NC_002947/1380086‑1380363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑