Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,380,722 G→T 10.8% intergenic (‑110/+25) PP_t67 ← / ← PP_t66 tRNA‑Met/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,380,7220GT10.8% 107.3 / 4.6 37intergenic (‑110/+25)PP_t67/PP_t66tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base G (13/20);  new base T (2/2);  total (15/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.57e-01

TTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTA  >  NC_002947/1380574‑1380869
                                                                                                                                                    |                                                                                                                                                    
ttATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACtgtg                                                                                                                                                      <  3:191956/149‑1 (MQ=255)
     aGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTaa                                                                                                                                                  <  7:178721/148‑1 (MQ=255)
        ccGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGT‑GTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTg                                                                                                                                             <  5:40472/149‑1 (MQ=255)
         cgacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTg                                                                                                                                             <  7:55706/149‑1 (MQ=255)
           acgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACACAGTGGc                                                                                                                                           <  3:113181/149‑1 (MQ=255)
           acgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGc                                                                                                                                           <  1:175702/149‑1 (MQ=255)
              aGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑tt                                                                                                                                       <  4:156823/149‑1 (MQ=255)
                  aCCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACa                                                                                                                                                >  6:76905/1‑137 (MQ=255)
                  aCCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACa                                                                                                                                                <  5:76905/137‑1 (MQ=255)
                   ccAGGCTGCTCCATCCCGAGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCtt                                                                                                                                  >  8:217884/1‑149 (MQ=255)
                     aGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGa                                                                                                                                <  8:179732/149‑1 (MQ=255)
                            tCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAg                                                                                                                                               <  2:322175/128‑1 (MQ=255)
                            tCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAg                                                                                                                                               >  1:322175/1‑128 (MQ=255)
                             ccATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTgg                                                                                                                          >  8:105952/1‑147 (MQ=255)
                             ccATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTgg                                                                                                                          <  7:105952/147‑1 (MQ=255)
                                  ccGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCgg                                                                                                                    <  7:167559/148‑1 (MQ=255)
                                   cgcgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCgg                                                                                                                    >  6:307270/1‑147 (MQ=255)
                                     cgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGc                                                                                                                <  5:307270/149‑1 (MQ=255)
                                          gTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGAt                                                                                                           <  3:165851/149‑1 (MQ=255)
                                            ggggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGTCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGAtt                                                                                                          <  7:217884/148‑1 (MQ=255)
                                                              tgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                        <  1:37521/148‑1 (MQ=255)
                                                                  gTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                                   >  2:48202/1‑149 (MQ=255)
                                                                          gTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcga                                                                           >  5:96485/1‑149 (MQ=255)
                                                                                   tAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTAc                                                                   >  5:126865/1‑148 (MQ=255)
                                                                                           gATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctgc                                                           <  1:48202/148‑1 (MQ=255)
                                                                                                              gcgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTgggg                                       <  6:126865/149‑1 (MQ=255)
                                                                                                                    gCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTAcc                                                                  >  7:195916/1‑116 (MQ=255)
                                                                                                                    gCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTAcc                                                                  <  8:195916/116‑1 (MQ=255)
                                                                                                                     ctaaTCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTTCCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGCATATGACCCAGACGAGCAACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGAta                                 >  2:97408/4‑147 (MQ=17)
                                                                                                                      ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGccc                                                                             >  7:124923/1‑103 (MQ=255)
                                                                                                                      ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGccc                                                                             <  8:124923/103‑1 (MQ=255)
                                                                                                                                  aaaGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGt                   >  1:13601/1‑149 (MQ=255)
                                                                                                                                       aaaaGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTtata              <  6:96485/149‑1 (MQ=255)
                                                                                                                                           ggCCACTGTTAACACAGTGGCCTTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGt           >  7:184281/1‑149 (MQ=17)
                                                                                                                                           ggCCACTGTTAACACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGa                                   <  8:252530/124‑1 (MQ=255)
                                                                                                                                           ggCCACTGTTAACACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGa                                   >  7:252530/1‑124 (MQ=255)
                                                                                                                                              cACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTAc                             >  5:75132/1‑127 (MQ=255)
                                                                                                                                              cACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTAc                             <  6:75132/127‑1 (MQ=255)
                                                                                                                                                   tgtTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTa  <  2:13601/149‑1 (MQ=255)
                                                                                                                                                    gtTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAG‑CCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGt                   <  3:288555/130‑1 (MQ=255)
                                                                                                                                                    gtTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAG‑CCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGt                   >  4:288555/1‑130 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTA  >  NC_002947/1380574‑1380869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑